scholarly journals A Complex Endomembrane System in the Archaeon Ignicoccus hospitalis Tapped by Nanoarchaeum equitans

2017 ◽  
Vol 8 ◽  
Author(s):  
Thomas Heimerl ◽  
Jennifer Flechsler ◽  
Carolin Pickl ◽  
Veronika Heinz ◽  
Benjamin Salecker ◽  
...  
Keyword(s):  
Endomembrane System ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

scholarly journals Proteomic Characterization of Cellular and Molecular Processes that Enable the Nanoarchaeum equitans-Ignicoccus hospitalis Relationship

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e22942 ◽  
Author(s):  
Richard J. Giannone ◽  
Harald Huber ◽  
Tatiana Karpinets ◽  
Thomas Heimerl ◽  
Ulf Küper ◽  
...  
Keyword(s):  
Molecular Processes ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

scholarly journals Ignicoccus hospitalis sp. nov., the host of ‘Nanoarchaeum equitans’

2007 ◽  
Vol 57 (4) ◽  
pp. 803-808 ◽  
Author(s):  
Walter Paper ◽  
Ulrike Jahn ◽  
Michael J. Hohn ◽  
Michaela Kronner ◽  
Daniela J. Näther ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Protein Composition ◽  
High Similarity ◽  
The North ◽  
Physiological Properties ◽  
Cell Architecture ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Unique Cell

A novel chemolithoautotrophic and hyperthermophilic member of the genus Ignicoccus was isolated from a submarine hydrothermal system at the Kolbeinsey Ridge, to the north of Iceland. The new isolate showed high similarity to the two species described to date, Ignicoccus islandicus and Ignicoccus pacificus, in its physiological properties as well as in its unique cell architecture. However, phylogenetic analysis and investigations on the protein composition of the outer membrane demonstrated that the new isolate was clearly distinct from I. islandicus and I. pacificus. Furthermore, it is the only organism known so far which is able to serve as a host for ‘Nanoarchaeum equitans’, the only cultivated member of the ‘Nanoarchaeota’. Therefore, the new isolate represents a novel species of the genus Ignicoccus, which we name Ignicoccus hospitalis sp. nov. (type strain KIN4/IT=DSM 18386T=JCM 14125T).

The interaction of Nanoarchaeum equitans with Ignicoccus hospitalis: proteins in the contact site between two cells

2009 ◽  
Vol 37 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Tillmann Burghardt ◽  
Benjamin Junglas ◽  
Frank Siedler ◽  
Reinhard Wirth ◽  
Harald Huber ◽  
...  
Keyword(s):  
Protein Complex ◽  
Direct Contact ◽  
Cell Surfaces ◽  
Contact Site ◽  
Electron Microscopic ◽  
Intimate Association ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Cell Envelopes ◽  
Microscopic Investigations

The two archaea Ignicoccus hospitalis and Nanoarchaeum equitans form a unique intimate association, the character of which is not yet fully understood. Electron microscopic investigations show that at least two modes of cell–cell interactions exist: (i) the two cells are interconnected via thin fibres; and (ii) the two cell surfaces are in direct contact with each other. In order to shed further light on the molecules involved, we isolated a protein complex, by using detergent-induced solubilization of cell envelopes, followed by a combination of chromatography steps. Analysis by MS and comparison with databases revealed that this fraction contained two dominant proteins, representing the respective major envelope proteins of the two archaea. In addition, a considerable set of membrane proteins is specifically associated with these proteins. They are now the focus of further biochemical and ultrastructural investigations.

Insights into the Structures of Superoxide Reductases from the Symbionts Ignicoccus hospitalis and Nanoarchaeum equitans

Biochemistry ◽  
2018 ◽  
Vol 57 (36) ◽  
pp. 5271-5281 ◽  
Author(s):  
Célia V. Romão ◽  
Pedro M. Matias ◽  
Cristiana M. Sousa ◽  
Filipa G. Pinho ◽  
Ana F. Pinto ◽  
...  
Keyword(s):  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

scholarly journals Life on the edge: functional genomic response of Ignicoccus hospitalis to the presence of Nanoarchaeum equitans

2014 ◽  
Vol 9 (1) ◽  
pp. 101-114 ◽  
Author(s):  
Richard J Giannone ◽  
Louie L Wurch ◽  
Thomas Heimerl ◽  
Stanton Martin ◽  
Zamin Yang ◽  
...  
Keyword(s):  
Functional Genomic ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Genomic Response

scholarly journals A genomic analysis of the archaeal system Ignicoccus hospitalis-Nanoarchaeum equitans

2008 ◽  
Vol 9 (11) ◽  
Author(s):  
Mircea Podar ◽  
Iain Anderson ◽  
Kira S Makarova ◽  
James G Elkins ◽  
Natalia Ivanova ◽  
...  
Keyword(s):  
Genomic Analysis ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

scholarly journals The hyperthermophilic partners Nanoarchaeum and Ignicoccus stabilize their tRNA T-loops via different but structurally equivalent modifications

2020 ◽  
Vol 48 (12) ◽  
pp. 6906-6918 ◽  
Author(s):  
Simon Rose ◽  
Sylvie Auxilien ◽  
Jesper F Havelund ◽  
Finn Kirpekar ◽  
Harald Huber ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tertiary Structure ◽  
Thiol Groups ◽  
Anti Conformation ◽  
Identical Manner ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans

Abstract The universal L-shaped tertiary structure of tRNAs is maintained with the help of nucleotide modifications within the D- and T-loops, and these modifications are most extensive within hyperthermophilic species. The obligate-commensal Nanoarchaeum equitans and its phylogenetically-distinct host Ignicoccus hospitalis grow physically coupled under identical hyperthermic conditions. We report here two fundamentally different routes by which these archaea modify the key conserved nucleotide U54 within their tRNA T-loops. In N. equitans, this nucleotide is methylated by the S-adenosylmethionine-dependent enzyme NEQ053 to form m5U54, and a recombinant version of this enzyme maintains specificity for U54 in Escherichia coli. In N. equitans, m5U54 is subsequently thiolated to form m5s2U54. In contrast, I. hospitalis isomerizes U54 to pseudouridine prior to methylating its N1-position and thiolating the O4-position of the nucleobase to form the previously uncharacterized nucleotide m1s4Ψ. The methyl and thiol groups in m1s4Ψ and m5s2U are presented within the T-loop in a spatially identical manner that stabilizes the 3′-endo-anti conformation of nucleotide-54, facilitating stacking onto adjacent nucleotides and reverse-Hoogsteen pairing with nucleotide m1A58. Thus, two distinct structurally-equivalent solutions have evolved independently and convergently to maintain the tertiary fold of tRNAs under extreme hyperthermic conditions.

scholarly journals Nanoarchaeum equitans and Ignicoccus hospitalis: New Insights into a Unique, Intimate Association of Two Archaea

2007 ◽  
Vol 190 (5) ◽  
pp. 1743-1750 ◽  
Author(s):  
Ulrike Jahn ◽  
Martin Gallenberger ◽  
Walter Paper ◽  
Benjamin Junglas ◽  
Wolfgang Eisenreich ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Mutual Influence ◽  
Strong Dependence ◽  
Gas Stripping ◽  
Electron Microscopic ◽  
Intimate Association ◽  
Cell Components ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans ◽  
Structural Connection

ABSTRACT Nanoarchaeum equitans and Ignicoccus hospitalis represent a unique, intimate association of two archaea. Both form a stable coculture which is mandatory for N. equitans but not for the host I. hospitalis. Here, we investigated interactions and mutual influence between these microorganisms. Fermentation studies revealed that during exponential growth only about 25% of I. hospitalis cells are occupied by N. equitans cells (one to three cells). The latter strongly proliferate in the stationary phase of I. hospitalis, until 80 to 90% of the I. hospitalis cells carry around 10 N. equitans cells. Furthermore, the expulsion of H2S, the major metabolic end product of I. hospitalis, by strong gas stripping yields huge amounts of free N. equitans cells. N. equitans had no influence on the doubling times, final cell concentrations, and growth temperature, pH, or salt concentration ranges or optima of I. hospitalis. However, isolation studies using optical tweezers revealed that infection with N. equitans inhibited the proliferation of individual I. hospitalis cells. This inhibition might be caused by deprivation of the host of cell components like amino acids, as demonstrated by 13C-labeling studies. The strong dependence of N. equitans on I. hospitalis was affirmed by live-dead staining and electron microscopic analyses, which indicated a tight physiological and structural connection between the two microorganisms. No alternative hosts, including other Ignicoccus species, were accepted by N. equitans. In summary, the data show a highly specialized association of N. equitans and I. hospitalis which so far cannot be assigned to a classical symbiosis, commensalism, or parasitism.

scholarly journals Multi-omics analysis provides insight to the Ignicoccus hospitalis-Nanoarchaeum equitans association

2017 ◽  
Vol 1861 (9) ◽  
pp. 2218-2227 ◽  
Author(s):  
Rachel A. Rawle ◽  
Timothy Hamerly ◽  
Brian P. Tripet ◽  
Richard J. Giannone ◽  
Louie Wurch ◽  
...  
Keyword(s):  
Omics Analysis ◽  
Ignicoccus Hospitalis ◽  
Nanoarchaeum Equitans
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