scholarly journals High Prevalence of Macrolide-resistance and Molecular Characterization of Streptococcus pyogenes Isolates Circulating in China from 2009 to 2016

2017 ◽  
Vol 8 ◽  
Author(s):  
Binghuai Lu ◽  
Yujie Fang ◽  
Yanyan Fan ◽  
Xingchun Chen ◽  
Junrui Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes ◽  
Macrolide Resistance ◽  
High Prevalence

scholarly journals Molecular characterization of Cronobacter sakazakii isolated from different herbal teas and mixtures in Serbia

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 921-934
Author(s):  
Dragana Josic ◽  
Marija Stojanovic ◽  
Zorica Lepsanovic ◽  
Vera Katic
Keyword(s):  
Molecular Characterization ◽  
Infant Formula ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii ◽  
Safety Risks ◽  
High Prevalence ◽  
Sequences Analysis ◽  
Human Infections ◽  
Rrna Sequences

Cronobacter sakazakii is an important cause of human infections that can be serious and even fatal among premature neonates and immunocompromised adults or infants. Because of its high tolerance to osmotic stress, C. sakazakii is frequently isolated from dried foods, such as powdered infant formula and herbal teas. The aim of investigation was detection, identification and molecular characterization of Cronobacter sakazakii isolates from infant formula and various herbal teas collected from Serbian market and tested for import control. C. sakazakii was not detected in any of the 360 analysed samples of powdered infant formula. However, 192 out of 520 samples of herbal teas tested were positive for C. sakazakii (37.1%). The high prevalence was observed in teas for children (51.6%) and in ?baby? teas (44.1%), followed by medicinal teas (38%). The largest one-herb-teas group (221 samples) contained 72 C. sakazakii-positive samples (32.6%) and involved Sennae folium, Althaeae radix, Menthae piperitae folium, Chamomilae flos and Urticae folium teas. Molecular characterization of isolated C. sakazakii from different herbal teas by rep-PCR, RAPD and 16S rRNA sequences analysis showed the high similarity to C. sakazakii NCTC 8155. Knowing this strain as one of the most pathogenic clinical strains, our results raise concern about the safety risks these foods pose to immunocompromised and healthy consumers, especially for babies and children.

Molecular Characterization of Streptococcus pyogenes Causing Invasive Disease in Pediatric Population in Spain A 12-year Study

2019 ◽  
Vol 38 (12) ◽  
pp. 1168-1172
Author(s):  
Viviana Sánchez-Encinales ◽  
Guillermo Ludwig ◽  
Esther Tamayo ◽  
Jose Maria García-Arenzana ◽  
Carmen Muñoz-Almagro ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes ◽  
Pediatric Population ◽  
Invasive Disease

scholarly journals Phenotypic and Genotypic Characterization of Streptococcus pyogenes Isolates Displaying the MLSB Phenotype of Macrolide Resistance in Spain, 1999 to 2005

2007 ◽  
Vol 51 (4) ◽  
pp. 1228-1233 ◽  
Author(s):  
Emilio Pérez-Trallero ◽  
Milagrosa Montes ◽  
Beatriz Orden ◽  
Esther Tamayo ◽  
José M. García-Arenzana ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Annual Variation ◽  
Macrolide Resistance ◽  
Resistant Clone ◽  
Erythromycin Resistance ◽  
Genetic Characteristics ◽  
Group A ◽  
Rapid Diffusion ◽  
Phenotypic And Genotypic Characterization

ABSTRACT The aim of this study was to describe the genetic characteristics of Streptococcus pyogenes showing the MLSB phenotype of macrolide resistance from 1999 to 2005 in Spain and to highlight the substantial increase in these isolates in the last few years. The antimicrobial susceptibilities of 17,232 group A streptococci isolated from Madrid and Gipuzkoa from 1999 to 2005 were studied. The presence of the resistance genes ermA, ermB, mef, tetM, and tetO and the presence of the intTn and xis genes of the Tn916-Tn1545 transposon family were studied in a sample of 739 MLSB-resistant isolates. The epidemiological relationships among these isolates were analyzed by emm typing, T typing, and multilocus sequence typing. Erythromycin resistance was found in 21.3% of the isolates analyzed (annual variation of 14.3% to 28.9%). Until 2003, most erythromycin-resistant isolates showed the M phenotype, but in 2004 and 2005, about 50% of isolates showed the MLSB phenotype. Among the MLSB-resistant isolates studied, 16 clones were identified. The most prevalent clone was a strange emm11/T11/ST403 clone with a null yqiL allele. All but one of the 463 emm11/T11/ST403 isolates carried the ermB, tetM, intTn, and xis genes. The second most prevalent MLSB-resistant clone was emm28/T28/ST52, which comprised two subclones: one bacitracin-resistant, tetracycline-susceptible subclone carrying the ermB gene (n = 115) and another bacitracin-susceptible, tetracycline-resistant subclone carrying the ermB and tetM genes (n = 33). The rapid diffusion of these two clones, and especially of emm11/T11/ST403, caused the large increase in MLSB-resistant S. pyogenes isolates in Spain, suggesting a potential ability for international dissemination.

Molecular characterization of macrolide resistant Streptococcus pyogenes isolates from pharyngitis patients in Serbia

2015 ◽  
Vol 33 ◽  
pp. 246-252 ◽  
Author(s):  
Natasa Opavski ◽  
Ina Gajic ◽  
Anna L. Borek ◽  
Katarzyna Obszańska ◽  
Maja Stanojevic ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes

scholarly journals Epidemiology and Molecular Characterization of Macrolide-Resistant Streptococcus pyogenes in Taiwan

2013 ◽  
Vol 52 (2) ◽  
pp. 508-516 ◽  
Author(s):  
C.-Y. Huang ◽  
J.-F. Lai ◽  
I.-W. Huang ◽  
P.-C. Chen ◽  
H.-Y. Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Streptococcus Pyogenes
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