scholarly journals Electrochemical Potential Influences Phenazine Production, Electron Transfer and Consequently Electric Current Generation by Pseudomonas aeruginosa

2017 ◽  
Vol 8 ◽  
Author(s):  
Erick M. Bosire ◽  
Miriam A. Rosenbaum
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transfer ◽  
Electric Current ◽  
Electrochemical Potential ◽  
Current Generation ◽  
Phenazine Production

scholarly journals Colour Me Blue: The History and the Biotechnological Potential of Pyocyanin

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 927
Author(s):  
Thiago Gonçalves ◽  
Ulrich Vasconcelos
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Therapy ◽  
Electric Current ◽  
Antimicrobial Therapy ◽  
Blue Colour ◽  
Current Generation ◽  
Biotechnological Potential ◽  
Avant Garde ◽  
New Horizons ◽  
The Subject

Pyocyanin was the first natural phenazine described. The molecule is synthesized by about 95% of the strains of Pseudomonas aeruginosa. From discovery up to now, pyocyanin has been characterised by a very rich and avant-garde history, which includes its use in antimicrobial therapy, even before the discovery of penicillin opened the era of antibiotic therapy, as well as its use in electric current generation. Exhibiting an exuberant blue colour and being easy to obtain, this pigment is the subject of the present review, aiming to narrate its history as well as to unveil its mechanisms and suggest new horizons for applications in different areas of engineering, biology and biotechnology.

scholarly journals Strain- and Substrate-Dependent Redox Mediator and Electricity Production by Pseudomonas aeruginosa

2016 ◽  
Vol 82 (16) ◽  
pp. 5026-5038 ◽  
Author(s):  
Erick M. Bosire ◽  
Lars M. Blank ◽  
Miriam A. Rosenbaum
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transfer ◽  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Pure Culture ◽  
Bioelectrochemical Systems ◽  
Content Type ◽  
Mediated Electron Transfer ◽  
Phenazine Production ◽  
Model Strain

ABSTRACTPseudomonas aeruginosais an important, thriving member of microbial communities of microbial bioelectrochemical systems (BES) through the production of versatile phenazine redox mediators. Pure culture experiments with a model strain revealed synergistic interactions ofP. aeruginosawith fermenting microorganisms whereby the synergism was mediated through the shared fermentation product 2,3-butanediol. Our work here shows that the behavior and efficiency ofP. aeruginosain mediated current production is strongly dependent on the strain ofP. aeruginosa. We compared levels of phenazine production by the previously investigated model strainP. aeruginosaPA14, the alternative model strainP. aeruginosaPAO1, and the BES isolatePseudomonassp. strain KRP1 with glucose and the fermentation products 2,3-butanediol and ethanol as carbon substrates. We found significant differences in substrate-dependent phenazine production and resulting anodic current generation for the three strains, with the BES isolate KRP1 being overall the best current producer and showing the highest electrochemical activity with glucose as a substrate (19 μA cm−2with ∼150 μg ml−1phenazine carboxylic acid as a redox mediator). Surprisingly,P. aeruginosaPAO1 showed very low phenazine production and electrochemical activity under all tested conditions.IMPORTANCEMicrobial fuel cells and other microbial bioelectrochemical systems hold great promise for environmental technologies such as wastewater treatment and bioremediation. While there is much emphasis on the development of materials and devices to realize such systems, the investigation and a deeper understanding of the underlying microbiology and ecology are lagging behind. Physiological investigations focus on microorganisms exhibiting direct electron transfer in pure culture systems. Meanwhile, mediated electron transfer with natural redox compounds produced by, for example,Pseudomonas aeruginosamight enable an entire microbial community to access a solid electrode as an alternative electron acceptor. To better understand the ecological relationships between mediator producers and mediator utilizers, we here present a comparison of the phenazine-dependent electroactivities of threePseudomonasstrains. This work forms the foundation for more complex coculture investigations of mediated electron transfer in microbial fuel cells.

Quorum sensing regulates electric current generation of Pseudomonas aeruginosa PA14 in bioelectrochemical systems

2010 ◽  
Vol 12 (3) ◽  
pp. 459-462 ◽  
Author(s):  
Arvind Venkataraman ◽  
Miriam Rosenbaum ◽  
Jan B.A. Arends ◽  
Rayko Halitschke ◽  
Largus T. Angenent
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Electric Current ◽  
Bioelectrochemical Systems ◽  
Current Generation

scholarly journals Gyrokinetic simulations of electric current generation in ion temperature gradient driven turbulence

2021 ◽  
Vol 28 (11) ◽  
pp. 112303
Author(s):  
Xiang Chen ◽  
Zhixin Lu ◽  
Huishan Cai ◽  
Lei Ye ◽  
Yang Chen ◽  
...  
Keyword(s):  
Temperature Gradient ◽  
Electric Current ◽  
Ion Temperature ◽  
Ion Temperature Gradient ◽  
Current Generation ◽  
Gyrokinetic Simulations

scholarly journals Cytochrome c550 from Pseudomonas aeruginosa

1993 ◽  
Vol 289 (1) ◽  
pp. 173-178 ◽  
Author(s):  
P Reichmann ◽  
H Görisch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transfer ◽  
Oxygen Consumption ◽  
Electron Transport ◽  
Electron Transport Chain ◽  
Prosthetic Group ◽  
Midpoint Potential ◽  
Transport Chain ◽  
Ethanol Dehydrogenase ◽  
Soluble C

In cells of Pseudomonas aeruginosa A.T.C.C. 17933 grown on ethanol the synthesis of a soluble c-type cytochrome, together with quinoprotein ethanol dehydrogenase, is induced. The cytochrome, with an alpha-absorption band at 550 nm, was purified to homogeneity. The molecular mass of the monomeric protein is 15 kDa, the pI is 4.8, and it contains one haem prosthetic group. The midpoint potential of the autoxidizable, but not autoreducible, cytochrome is 280 mV. Cytochrome c550 mediates electron transfer between quinoprotein ethanol dehydrogenase and ferricyanide. In a system composed of membrane particles with NN‘NN’-tetramethyl-p-phenylenediamine oxidase activity and quinoprotein ethanol dehydrogenase, oxygen consumption is only observed in the presence of cytochrome c550. This indicates the participation of the cytochrome in the electron-transport chain linked to quinoprotein ethanol dehydrogenase in P. aeruginosa. The electron transport from ethanol dehydrogenase to oxygen is inhibited by myxothiazol and antimycin, indicating that a cytochrome bc1-like complex is involved.

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