scholarly journals Biocontrol of Sugarcane Smut Disease by Interference of Fungal Sexual Mating and Hyphal Growth Using a Bacterial Isolate

2017 ◽  
Vol 8 ◽  
Author(s):  
Shiyin Liu ◽  
Nuoqiao Lin ◽  
Yumei Chen ◽  
Zhibin Liang ◽  
Lisheng Liao ◽  
...  
Keyword(s):  
Bacterial Isolate ◽  
Hyphal Growth ◽  
Sexual Mating

scholarly journals Pseudomonas sp. ST 4 produces variety of active compounds to interfere fungal sexual mating and hyphal growth

2020 ◽  
Vol 13 (1) ◽  
pp. 107-117 ◽  
Author(s):  
Shiyin Liu ◽  
Fei He ◽  
Nuoqiao Lin ◽  
Yumei Chen ◽  
Zhibin Liang ◽  
...  
Keyword(s):  
Hyphal Growth ◽  
Pseudomonas Sp ◽  
Active Compounds ◽  
Sexual Mating

Microscopy studies of powdery mildew on summer squash

1991 ◽  
Vol 49 ◽  
pp. 520-521
Author(s):  
John S. Gardner ◽  
W. M. Hess
Keyword(s):  
Powdery Mildew ◽  
Tip Growth ◽  
Hyphal Growth ◽  
Plant Tissues ◽  
Vegetative Hypha ◽  
Powdery Mildews ◽  
Summer Squash ◽  
Conidial Formation ◽  
Polar Tip Growth ◽  
Short Conidiophore

Powdery mildews are characterized by the appearance of spots or patches of a white to grayish, powdery, mildewy growth on plant tissues, entire leaves or other organs. Ervsiphe cichoracearum, the powdery mildew of cucurbits is among the most serious parasites, and the most common. The conidia are formed similar to the process described for Ervsiphe graminis by Cole and Samson. Theconidial chains mature basipetally from a short, conidiophore mother-cell at the base of the fertile hypha which arises holoblastically from the conidiophore. During early development it probably elongates by polar-tip growth like a vegetative hypha. A septum forms just above the conidiophore apex. Additional septa develop in acropetal succession. However, the conidia of E. cichoracearum are more doliform than condia from E. graminis. The purpose of these investigations was to use scanning electron microscopy (SEM) to demonstrate the nature of hyphal growth and conidial formation of E. cichoracearum on field-grown squash leaves.

Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition

2020 ◽  
Vol 21 (13) ◽  
pp. 1304-1315
Author(s):  
Junmei Zhou ◽  
Lianghong Yin ◽  
Chenbin Wu ◽  
Sijia Wu ◽  
Jidong Lu ◽  
...  
Keyword(s):  
Bacterial Isolate ◽  
Ammonium Oxalate ◽  
Fermentation Condition ◽  
Bivalent Cations ◽  
Rdna Sequencing ◽  
Optimum Ph ◽  
Alkaline Conditions ◽  
Fermentation Parameters ◽  
Pulp And Paper Industries ◽  
Central Composite

Objective: Alkaline Carboxymethyl Cellulase (CMCase) is an attractive enzyme for the textile, laundry, pulp, and paper industries; however, commercial preparations with sufficient activity at alkaline conditions are scarce. Methods: High CMCase-producing bacterial isolate, SX9-4, was screened out from soil bacteria, which was identified as Flavobacterium sp. on the basis of 16S rDNA sequencing. Results: The optimum pH and temperature for CMCase reaction were 8.0 and 55°C, respectively. Alkaline CMCase was stable over wide pH (3.0-10.6) and temperature (25-55°C) ranges. Enzyme activity was significantly inhibited by the bivalent cations Mn2+ and Cu2+, and was activated by Fe2+. To improve the alkaline CMCase production of SX9-4, fermentation parameters were selected through onefactor- at-a-time and further carried out by response surface methodologies based on a central composite design. Conclusion: High CMCase production (57.18 U/mL) was achieved under the optimal conditions: 10.53 g/L carboxymethylcellulose sodium, 7.74 g/L glucose, 13.71 g/L peptone, and 5.27 g/L ammonium oxalate.

scholarly journals Elevated Sporulation Efficiency in Fission Yeast Schizosaccharomyces japonicus Strains Isolated from Drosophila

2021 ◽  
Vol 7 (5) ◽  
pp. 350
Author(s):  
Taisuke Seike ◽  
Natsue Sakata ◽  
Fumio Matsuda ◽  
Chikara Furusawa
Keyword(s):  
Signal Transduction ◽  
Fission Yeast ◽  
High Frequency ◽  
Type Strain ◽  
Hyphal Growth ◽  
Amino Acid Sequences ◽  
Iodine Vapor ◽  
Sporulation Efficiency ◽  
Yeast Ecology ◽  
Wild Strains

The fission yeast Schizosaccharomyces japonicus, comprising S. japonicus var. japonicus and S. japonicus var. versatilis varieties, has unique characteristics such as striking hyphal growth not seen in other Schizosaccharomyces species; however, information on its diversity and evolution, in particular mating and sporulation, remains limited. Here we compared the growth and mating phenotypes of 17 wild strains of S. japonicus, including eight S. japonicus var. japonicus strains newly isolated from an insect (Drosophila). Unlike existing wild strains isolated from fruits/plants, the strains isolated from Drosophila sporulated at high frequency even under nitrogen-abundant conditions. In addition, one of the strains from Drosophila was stained by iodine vapor, although the type strain of S. japonicus var. japonicus is not stained. Sequence analysis further showed that the nucleotide and amino acid sequences of pheromone-related genes have diversified among the eight strains from Drosophila, suggesting crossing between S. japonicus cells of different genetic backgrounds occurs frequently in this insect. Much of yeast ecology remains unclear, but our findings suggest that insects such as Drosophila might be a good niche for mating and sporulation, and will provide a basis for the understanding of sporulation mechanisms via signal transduction, as well as the ecology and evolution of yeast.

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