scholarly journals A Small Regulatory RNA Contributes to the Preferential Colonization of Escherichia coli O157:H7 in the Large Intestine in Response to a Low DNA Concentration

2017 ◽  
Vol 8 ◽  
Author(s):  
Runhua Han ◽  
Letian Xu ◽  
Ting Wang ◽  
Bin Liu ◽  
Lei Wang
Keyword(s):  
Escherichia Coli ◽  
Large Intestine ◽  
Escherichia Coli O157 ◽  
Regulatory Rna ◽  
Dna Concentration ◽  
Small Regulatory Rna

Mo1770 Expression of the Oxys Small Regulatory RNA in Luminal Commensal Escherichia coli Attenuates Experimental Colitis

2012 ◽  
Vol 142 (5) ◽  
pp. S-681
Author(s):  
Sandrine Y. Tchaptchet ◽  
Ting-Jia Fan ◽  
Laura E. Goeser ◽  
Ryan B. Sartor ◽  
Jonathan J. Hansen
Keyword(s):  
Escherichia Coli ◽  
Experimental Colitis ◽  
Regulatory Rna ◽  
Small Regulatory Rna

A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli

2014 ◽  
Vol 60 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Naoki Sudo ◽  
Akiko Soma ◽  
Akira Muto ◽  
Sunao Iyoda ◽  
Mayumi Suh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Motility ◽  
Enterohemorrhagic Escherichia Coli ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli

2012 ◽  
Vol 84 (1) ◽  
pp. 51-65 ◽  
Author(s):  
Franziska Mika ◽  
Susan Busse ◽  
Alexandra Possling ◽  
Janine Berkholz ◽  
Natalia Tschowri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stationary Phase ◽  
Biofilm Formation ◽  
Cell Envelope ◽  
Regulatory Rna ◽  
Small Regulatory Rna ◽  
Envelope Stress

The Escherichia Coli Small Regulatory Rna Oxys Impairs Bacterial Translocation and Attenuates Experimental Colitis

2017 ◽  
Vol 152 (5) ◽  
pp. S1000
Author(s):  
Diana Arsene ◽  
Sandrine Y. Tchaptchet ◽  
Ting-Jia Fan ◽  
Jonathan J. Hansen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Translocation ◽  
Experimental Colitis ◽  
Regulatory Rna ◽  
Small Regulatory Rna

Colonization of 8-week-old conventionally reared goats by Escherichia coli O157 : H7 after oral inoculation

2005 ◽  
Vol 54 (5) ◽  
pp. 485-492 ◽  
Author(s):  
R M La Ragione ◽  
N MY Ahmed ◽  
A Best ◽  
D Clifford ◽  
U Weyer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Large Intestine ◽  
Direct Contact ◽  
Distal Colon ◽  
Escherichia Coli O157 ◽  
Wild Type ◽  
E Coli ◽  
Goat’S Milk ◽  
Oral Inoculation ◽  
Strain Nctc

Enterohaemorrhagic Escherichia coli O157 : H7 infections of man have been associated with consumption of unpasteurized goat's milk and direct contact with kid goats on petting farms, yet little is known about colonization of goats with this organism. To assess the contribution of flagella and intimin of E. coli O157 : H7 in colonization of the goat, 8-week-old conventionally reared goats were inoculated orally in separate experiments with 1×1010 c.f.u. of a non-verotoxigenic strain of E. coli O157 : H7 (strain NCTC 12900 Nalr), an aflagellate derivative (DMB1) and an intimin-deficient derivative (DMB2). At 24 h after inoculation, the three E. coli O157 : H7 strains were shed at approximately 5×104 c.f.u. (g faeces)−1 from all animals. Significantly fewer intimin-deficient bacteria were shed only on days 2 (P = 0.003) and 4 (P = 0.014), whereas from day 7 to 29 there were no differences. Tissues from three animals inoculated with wild-type E. coli O157 : H7 strain NCTC 12900 Nalr were sampled at 24, 48 and 96 h after inoculation and the organism was cultured from the large intestine of all three animals and from the duodenum and ileum of the animal examined at 96 h. Tissues were examined histologically but attaching-effacing (AE) lesions were not observed at any intestinal site of the animals examined at 24 or 48 h. However, the animal examined at 96 h, which had uniquely shed approximately 1×107 E. coli O157 : H7 (g faeces)−1 for the preceding 3 days, showed a heavy, diffuse infection with cryptosporidia and abundant, multifocal AE lesions in the distal colon, rectum and at the recto-anal junction. These AE lesions were confirmed by immunohistochemistry to be associated with E. coli O157 : H7.

scholarly journals Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis

2022 ◽  
Author(s):  
Ben F Luisi ◽  
Md. Saiful Islam ◽  
Steven William Hardwick ◽  
Laura Quell ◽  
Dimitri Y Chirgadze ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Cell ◽  
Quaternary Structure ◽  
Cell Envelope ◽  
Evolutionary Relationship ◽  
Regulatory Rna ◽  
Rnase E ◽  
Small Regulatory Rna ◽  
Conserved Domain ◽  
Transcription Networks

The biogenesis of the essential precursor of the bacterial cell envelope, glucosamine-6-phosphate (GlcN6P), is controlled through intricate post-transcription networks mediated by GlmZ, a small regulatory RNA (sRNA). GlmZ stimulates translation of the mRNA encoding GlcN6P synthetase in Escherichia coli, but when bound by the protein RapZ, it becomes inactivated through cleavage by the endoribonuclease RNase E. Here we report the cryoEM structure of the RapZ:GlmZ complex, revealing a complementary match of the protein tetrameric quaternary structure to an imperfect structural repeat in the RNA. The RNA is contacted mostly through a highly conserved domain of RapZ that shares deep evolutionary relationship with phosphofructokinase and suggests links between metabolism and riboregulation. We also present the structure of a pre-cleavage encounter intermediate formed between the binary RapZ:GlmZ complex and RNase E that reveals how GlmZ is presented and recognised for cleavage. The structures suggest how other encounter complexes might guide recognition and action of endoribonucleases on target transcripts, and how structured substrates in polycistronic precursors are recognised for processing.

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