scholarly journals Analysis and Characterization of Proteins Associated with Outer Membrane Vesicles Secreted by Cronobacter spp.

2017 ◽  
Vol 8 ◽  
Author(s):  
Mahendra H. Kothary ◽  
Gopal R. Gopinath ◽  
Jayanthi Gangiredla ◽  
Prasad V. Rallabhandi ◽  
Lisa M. Harrison ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

Characterization of outer membrane vesicles of Acetobacter pasteurianus NBRC3283

2018 ◽  
Vol 125 (4) ◽  
pp. 425-431 ◽  
Author(s):  
Masahito Hashimoto ◽  
Taichi Matsumoto ◽  
Miwa Tamura-Nakano ◽  
Mami Ozono ◽  
Shuhei Hashiguchi ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Acetobacter Pasteurianus

scholarly journals Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori

2019 ◽  
Vol 34 (1) ◽  
pp. 189-195 ◽  
Author(s):  
Maurizio Ronci ◽  
Sonia Del Prete ◽  
Valentina Puca ◽  
Simone Carradori ◽  
Vincenzo Carginale ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Identification And Characterization

Proteomic and functional characterization of the outer membrane vesicles from the gastric pathogen Helicobacter pylori

2009 ◽  
Vol 3 (7) ◽  
pp. 785-796 ◽  
Author(s):  
Erica Mullaney ◽  
Paul A. Brown ◽  
Sinead M. Smith ◽  
Catherine H. Botting ◽  
Yoshio Y. Yamaoka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Outer Membrane ◽  
Functional Characterization ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Gastric Pathogen

Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles

Vaccine ◽  
2014 ◽  
Vol 32 (46) ◽  
pp. 6084-6090 ◽  
Author(s):  
Maximiliano Ormazábal ◽  
Erika Bartel ◽  
María Emilia Gaillard ◽  
Daniela Bottero ◽  
Agustina Errea ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Pertussis Vaccine ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles

scholarly journals Characterization of outer membrane vesicles released by the psychrotolerant bacterium Pseudoalteromonas antarctica NF3

2006 ◽  
Vol 8 (9) ◽  
pp. 1523-1533 ◽  
Author(s):  
Maria Nevot ◽  
Victor Deroncele ◽  
Paul Messner ◽  
Jesus Guinea ◽  
Elena Mercade
Keyword(s):  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Psychrotolerant Bacterium
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