scholarly journals Use of Random T-DNA Mutagenesis in Identification of Gene UvPRO1, A Regulator of Conidiation, Stress Response, and Virulence in Ustilaginoidea virens

2016 ◽  
Vol 7 ◽  
Author(s):  
Bo Lv ◽  
Lu Zheng ◽  
Hao Liu ◽  
Jintian Tang ◽  
Tom Hsiang ◽  
...  
Keyword(s):  
Stress Response ◽  
Ustilaginoidea Virens ◽  
Dna Mutagenesis

scholarly journals UvWhi2 Is Required for Stress Response and Pathogenicity in Ustilaginoidea virens

Rice Science ◽  
2022 ◽  
Vol 29 (1) ◽  
pp. 47-54
Author(s):  
Meng Shuai ◽  
Qiu Jiehua ◽  
Xiong Meng ◽  
Liu Zhiquan ◽  
Jane Sadhna Jagernath ◽  
...  
Keyword(s):  
Stress Response ◽  
Ustilaginoidea Virens

scholarly journals UvWHI2 is Required for Stress Response and Pathogenicity in Ustilaginonidea Virens

2020 ◽  
Author(s):  
Shuai Meng ◽  
Jiehua Qiu ◽  
Meng Xiong ◽  
Zhiquan Liu ◽  
Jane Sadhna Jagernath ◽  
...  
Keyword(s):  
Stress Response ◽  
Stress Responses ◽  
Fungal Growth ◽  
Growth Stress ◽  
Phenotypic Analysis ◽  
Ustilaginoidea Virens ◽  
General Stress Response ◽  
Relative Expression Level ◽  
False Smut ◽  
Limited Surface

Abstract Background: Ustilaginoidea virens causes rice false smut disease, which emerges as a worldwide disease of rice. At present, some stress response related genes have been identified in U. virens , but it is not clear whether and how defects of stress responses affect the pathogenesis processes of U. virens . To answer this question, the function of a general stress response factor Whi2 was analyzed in U. virens . Results: In this study, we identified UvWhi2 as a homolog of Saccharomyces cerevisiae Whi2 in U. virens. The relative expression level of UvWhi2 was significantly up-regulated during infection, suggesting that UvWhi2 may be involved in pathogenesis. Furthermore, knockout of UvWhi2 showed decreased the mycelial growth, increased in conidiation in the PS (potato sucrose) medium and a defect in pathogenicity. In addition, the RNA-Seq and phenotypic analysis showed that UvWHI2 is involved in response to oxidative, hyperosmotic, cell wall stresses, and nutrient limitation. Further studies revealed that the defects of stress responses of the ∆ Uvwhi2 mutant affected the formation of secondary spores on the nutrient limited surface and the rice surface, resulting in a significant reduction of pathogenicity of U. virens . Conclusions: Our results suggest that UvWhi2 is necessary for fungal growth, stress responses, and the formation of secondary spores in U. virens . In addition, the defects of stress responses could affect the formation of secondary spores on the rice surface, and then compromise the pathogenicity of U. virens .

scholarly journals UvKmt6-mediated H3K27 trimethylation is required for development, pathogenicity, and stress response in Ustilaginoidea virens

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 2972-2988
Author(s):  
Shuai Meng ◽  
Zhiquan Liu ◽  
Huanbin Shi ◽  
Zhongling Wu ◽  
Jiehua Qiu ◽  
...  
Keyword(s):  
Stress Response ◽  
Ustilaginoidea Virens ◽  
H3k27 Trimethylation

scholarly journals Posttranslational arginylation enzyme Ate1 affects DNA mutagenesis by regulating stress response

2016 ◽  
Vol 7 (9) ◽  
pp. e2378-e2378 ◽  
Author(s):  
Akhilesh Kumar ◽  
Michael D Birnbaum ◽  
Devang M Patel ◽  
William M Morgan ◽  
Jayanti Singh ◽  
...  
Keyword(s):  
Stress Response ◽  
Dna Mutagenesis

scholarly journals Transcription factor UvMsn2 is important for vegetative growth, conidiogenesis, stress response, mitochondrial morphology and pathogenicity in the rice false smut fungus Ustilaginoidea virens

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Yuandi Xu ◽  
Shuang Wu ◽  
Zhaomeng Yu ◽  
Emily Kolojane Moeketsi ◽  
Zhixiang Yang ◽  
...  
Keyword(s):  
Stress Response ◽  
Vegetative Growth ◽  
Rice Blast Fungus ◽  
Smut Fungus ◽  
Mitochondrial Morphology ◽  
Rice Seed ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
Aerial Hyphae ◽  
False Smut

AbstractTranscription factors (TFs) play critical roles in the control of development and pathogenicity of phytopathogens by directly or indirectly regulating the expression of downstream genes. Here, we identified and characterized a zinc finger TF UvMsn2 in Ustilaginoidea virens, a homolog of MoMsn2 from the rice blast fungus. Heterogenous complementation assay revealed that UvMsn2 fully restored the defects of the ∆Momsn2 mutant in vegetative growth, conidiation and pathogenicity. Deletion of UvMsn2 in U. virens led to a reduction of the pathogen in vegetative growth, aerial hyphae and conidiation. Additionally, the ∆Uvmsn2 mutant displayed defects in conidial morphology and germination, as well as mitochondrial morphology. Pathogenicity and toxicity assays revealed that the ∆Uvmsn2 mutant was non-pathogenic and less inhibitory to rice seed germination. The ∆Uvmsn2 mutant showed different sensitivity to various stresses. Further microscopic examination found that UvMsn2 was localized in both cytosol and nucleus, and translocated from cytosol to nucleus under the treatment of NaCl. Our results demonstrate that UvMsn2 is a critical TF that regulates the vegetative growth, conidiogenesis, stress response, mitochondrial morphology and virulence in the rice false smut fungus.

CarR, a MarR-family regulator from Corynebacterium glutamicum, modulated antibiotic and aromatic compound resistance

2019 ◽  
Vol 476 (21) ◽  
pp. 3141-3159 ◽  
Author(s):  
Meiru Si ◽  
Can Chen ◽  
Zengfan Wei ◽  
Zhijin Gong ◽  
GuiZhi Li ◽  
...  
Keyword(s):  
Stress Response ◽  
Ligand Binding ◽  
Corynebacterium Glutamicum ◽  
Conformational Changes ◽  
Cysteine Oxidation ◽  
Transcriptional Regulatory ◽  
Ligand Free ◽  
Redox Sensing

Abstract MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this study, we characterized the stress-related ligand-binding functions of the C. glutamicum MarR-type regulator CarR (C. glutamicum antibiotic-responding regulator). We demonstrate that CarR negatively regulates the expression of the carR (ncgl2886)–uspA (ncgl2887) operon and the adjacent, oppositely oriented gene ncgl2885, encoding the hypothetical deacylase DecE. We also show that CarR directly activates transcription of the ncgl2882–ncgl2884 operon, encoding the peptidoglycan synthesis operon (PSO) located upstream of carR in the opposite orientation. The addition of stress-associated ligands such as penicillin and streptomycin induced carR, uspA, decE, and PSO expression in vivo, as well as attenuated binding of CarR to operator DNA in vitro. Importantly, stress response-induced up-regulation of carR, uspA, and PSO gene expression correlated with cell resistance to β-lactam antibiotics and aromatic compounds. Six highly conserved residues in CarR were found to strongly influence its ligand binding and transcriptional regulatory properties. Collectively, the results indicate that the ligand binding of CarR induces its dissociation from the carR–uspA promoter to derepress carR and uspA transcription. Ligand-free CarR also activates PSO expression, which in turn contributes to C. glutamicum stress resistance. The outcomes indicate that the stress response mechanism of CarR in C. glutamicum occurs via ligand-induced conformational changes to the protein, not via cysteine oxidation-based thiol modifications.

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