scholarly journals Outer Membrane Vesicles and Soluble Factors Released by Probiotic Escherichia coli Nissle 1917 and Commensal ECOR63 Enhance Barrier Function by Regulating Expression of Tight Junction Proteins in Intestinal Epithelial Cells

2016 ◽  
Vol 7 ◽  
Author(s):  
Carina-Shianya Alvarez ◽  
Josefa Badia ◽  
Manel Bosch ◽  
Rosa Giménez ◽  
Laura Baldomà
Keyword(s):  
Escherichia Coli ◽  
Intestinal Epithelial Cells ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Intestinal Epithelial ◽  
Tight Junction Proteins ◽  
Soluble Factors ◽  
Escherichia Coli Nissle 1917 ◽  
Escherichia Coli Nissle ◽  
Junction Proteins

scholarly journals Strong Decrease in Invasive Ability and Outer Membrane Vesicle Release in Crohn's Disease-Associated Adherent-Invasive Escherichia coli Strain LF82 with the yfgL Gene Deleted

2005 ◽  
Vol 187 (7) ◽  
pp. 2286-2296 ◽  
Author(s):  
Nathalie Rolhion ◽  
Nicolas Barnich ◽  
Laurent Claret ◽  
Arlette Darfeuille-Michaud
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Outer Membrane ◽  
Culture Supernatant ◽  
Intestinal Epithelial Cells ◽  
Outer Membrane Proteins ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Intestinal Epithelial ◽  
Isogenic Mutant

ABSTRACT Adherent-invasive Escherichia coli strain LF82 recovered from a chronic lesion of a patient with Crohn's disease is able to invade cultured intestinal epithelial cells. Three mutants with impaired ability to invade epithelial cells had the Tn5phoA transposon inserted in the yfgL gene encoding the YfgL lipoprotein. A yfgL- negative isogenic mutant showed a marked decrease both in its ability to invade Intestine-407 cells and in the amount of the outer membrane proteins OmpA and OmpC in the culture supernatant, as shown by analysis of the culture supernatant protein contents by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Transcomplementation of the LF82-ΔyfgL isogenic mutant with the cloned yfgL gene restored invasion ability and outer membrane protein release in the culture supernatant. The outer membrane proteins in the culture supernatant of strain LF82 resulted from the formation of vesicles. This was shown by Western blot analysis of periplasmic and outer membrane fraction markers typically found in outer membrane vesicles and by transmission electron microscopic analysis of ultracentrifuged cell-free LF82 supernatant pellets, indicating the presence of vesicles with a bilayered structure surrounding a central electron-dense core. Thus, deletion of the yfgL gene in strain LF82 resulted in a decreased ability to invade intestinal epithelial cells and a decreased release of outer membrane vesicles.

Genome-wide identification of probiotic Escherichia coli Nissle 1917 (EcN) fitness genes during adhesion to the intestinal epithelial cells Caco-2

Gene ◽  
2021 ◽  
pp. 145890
Author(s):  
Sathyanarayanan Jayashree ◽  
Ramamoorthy Sivakumar ◽  
Raman Karthikeyan ◽  
Paramasamy Gunasekaran ◽  
Jeyaprakash Rajendhran
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Genome Wide ◽  
Escherichia Coli Nissle 1917 ◽  
Escherichia Coli Nissle
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