scholarly journals High Prevalence of β-lactamase and Plasmid-Mediated Quinolone Resistance Genes in Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Dogs in Shaanxi, China

2016 ◽  
Vol 7 ◽  
Author(s):  
Xiaoqiang Liu ◽  
Haixia Liu ◽  
Yinqian Li ◽  
Caiju Hao
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Quinolone Resistance ◽  
Extended Spectrum ◽  
High Prevalence

Trend of plasmid-mediated quinolone resistance genes at the Children’s Hospital in Tunisia

2014 ◽  
Vol 63 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Nour El-Houda Jlili ◽  
Samia Réjiba ◽  
Hanen Smaoui ◽  
Thomas Guillard ◽  
Françoise Chau ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Random Amplified Polymorphic Dna ◽  
Enterobacter Cloacae ◽  
Quinolone Resistance ◽  
Children's Hospital ◽  
Extended Spectrum ◽  
High Prevalence ◽  
Children’S Hospital ◽  
Dna Pcr

The prevalence of plasmid-mediated quinolone resistance genes [qnr, aac(6′)-Ib-cr and qepA] was sought among Enterobacteriaceae strains obtained from the Children’s Hospital of Tunis (Tunisia). Non-duplicate isolates (n = 278) with resistance to extended-spectrum cephalosporins and collected in 2003, 2007, 2008 and 2009 were screened for qnr genes. Forty (14.4 %) isolates were qnr positive and were screened for the presence of the aac(6′)-Ib-cr and qepA genes. qnrB was detected in 21 Klebsiella pneumoniae, 11 Escherichia coli and 6 Enterobacter cloacae isolates. Sequence analysis of the qnrB amplicons revealed variants including 24 qnrB1, 11 qnrB2 and 3 qnrB6. qnrS (qnrS1 allele) was detected only in K. pneumoniae isolates, either alone (two isolates) or with the qnrB gene (one isolate). The qnrA, qnrC and qnrD genes were not found in any of the 278 isolates. No qnr-positive isolates carried the qepA gene. Pyrosequencing results showed that aac(6′)-Ib-cr, a variant of the aac(6′)-Ib gene, was present in 31 qnr-positive isolates (21 K. pneumoniae isolates, seven Escherichia coli isolates and three Enterobacter cloacae isolates). aac(6′)-Ib was also found either alone (two isolates) or in association with aac(6′)-Ib-cr (one isolate). Of the 40 qnr-positive isolates, 92.5, 82.5, 57.5, 85 and 82.5 % were non-susceptible to nalidixic acid, ciprofloxacin, levofloxacin, ofloxacin and norfloxacin, respectively, and all were extended-spectrum β-lactamase producers. Random amplified polymorphic DNA-PCR typing of these isolates showed 16, 8 and 5 different genotypes in K. pneumoniae, Escherichia coli and Enterobacter cloacae isolates, respectively. Our study highlights the high prevalence of qnr in association with aac(6′)-Ib-cr among Enterobacteriaceae isolates, even from children, who are patients not overtreated with quinolones.

scholarly journals Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran

2015 ◽  
Vol 8 (12) ◽  
Author(s):  
Elnaz Harifi Mood ◽  
Zahra Meshkat ◽  
Nafiseh Izadi ◽  
Maryam Rezaei ◽  
Saeid Amel Jamehdar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Quinolone Resistance ◽  
Extended Spectrum

scholarly journals Emergence of the Quinolone Resistance-Mediating Gene aac(6′)-Ib-cr in Extended-Spectrum-β-Lactamase-Producing Klebsiella Isolates Collected in Slovenia between 2000 and 2005

2007 ◽  
Vol 51 (11) ◽  
pp. 4171-4173 ◽  
Author(s):  
Jerneja Ambrožič Avguštin ◽  
Rok Keber ◽  
Katja Žerjavič ◽  
Toni Oražem ◽  
Miklavž Grabnar
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Quinolone Resistance ◽  
Extended Spectrum

ABSTRACT Seventy-four nonrepetitive uropathogenic fluoroquinolone-resistant or -intermediate extended-spectrum-β-lactamase-producing Klebsiella isolates from Slovenia were screened for the presence of plasmid-mediated quinolone resistance genes. None of the known qnr genes were detected. The aac(6′)-Ib-cr allele was detected on plasmids from 25 transconjugants for which the ciprofloxacin MIC was higher than for the recipient Escherichia coli strain.

scholarly journals Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Shaymaa H. Abdel-Rhman ◽  
Rehab M. Elbargisy ◽  
Dina E. Rizk
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Efflux Pump ◽  
Quinolone Resistance ◽  
Aminoglycoside Resistance ◽  
Variable Regions ◽  
Active Efflux ◽  
E Coli ◽  
High Prevalence ◽  
Class I Integrons

Escherichia coli is a common pathogen in both humans and animals. Quinolones are used to treat infections caused by Gram-negative bacteria, but resistance genes emerged. Only scarce studies investigated the association between plasmid-mediated quinolone resistance (PMQR) genes and integrons in clinical isolates of E. coli. The current study investigated the prevalence of quinolone resistance and integrons among 134 clinical E. coli isolates. Eighty (59.70%) isolates were quinolone-resistant, and 60/134 (44.77%) isolates were integron positive with the predominance of class I integrons (98.33%). There was a significant association between quinolone resistance and the presence of integrons ( P < 0.0001 ). Isolates from Urology and Nephrology Center and Gastroenterology Hospital were significantly quinolone-resistant and integron positive ( P ≤ 0.0005 ). Detection of PMQR genes on plasmids of integron-positive isolates showed that the active efflux pump genes oqxAB and qepA had the highest prevalence (72.22%), followed by the aminoglycoside acetyltransferase gene (aac(6′)-Ib-cr, 66.67%) and the quinolone resistance genes (qnr, 61.11%). Amplification and sequencing of integrons’ variable regions illustrated that no quinolone resistance genes were detected, and the most predominant gene cassettes were for trimethoprim and aminoglycoside resistance including dfrA17, dfrB4, and dfrA17-aadA5. In conclusion, this study reported the high prevalence of PMQR genes and integrons among clinical E. coli isolates. Although PMQR genes are not cassette-born, they were associated with integrons’ presence, which contributes to the widespread of quinolone resistance in Egypt.

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