scholarly journals The Small RNA DsrA Influences the Acid Tolerance Response and Virulence of Salmonella enterica Serovar Typhimurium

2016 ◽  
Vol 7 ◽  
Author(s):  
Daniel Ryan ◽  
Urmesh K. Ojha ◽  
Sangeeta Jaiswal
Keyword(s):  
Small Rna ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Acid Tolerance ◽  
Acid Tolerance Response ◽  
Serovar Typhimurium ◽  
Tolerance Response

scholarly journals The Lactic Acid-Induced Acid Tolerance Response in Salmonella enterica Serovar Typhimurium Induces Sensitivity to Hydrogen Peroxide

2006 ◽  
Vol 72 (8) ◽  
pp. 5623-5625 ◽  
Author(s):  
E. J. Greenacre ◽  
S. Lucchini ◽  
J. C. D. Hinton ◽  
T. F. Brocklehurst
Keyword(s):  
Hydrogen Peroxide ◽  
Lactic Acid ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Acid Tolerance ◽  
Down Regulation ◽  
Acid Tolerance Response ◽  
Essential Component ◽  
Serovar Typhimurium ◽  
Tolerance Response

ABSTRACT Transcriptome analyses of Salmonella enterica serovar Typhimurium revealed that 15 genes were significantly up-regulated after 2 h of adaptation with lactic acid. cadB was the most highly up-regulated gene and was shown to be an essential component. Lactic acid-adapted cells exhibited sensitivity to hydrogen peroxide, likely due to down-regulation of the OxyR regulon.

scholarly journals CadC Has a Global Translational Effect during Acid Adaptation in Salmonella enterica Serovar Typhimurium

2007 ◽  
Vol 189 (6) ◽  
pp. 2417-2425 ◽  
Author(s):  
Yong Heon Lee ◽  
Bae Hoon Kim ◽  
Ji Hye Kim ◽  
Won Suck Yoon ◽  
Seong Ho Bang ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Regulatory System ◽  
Acid Tolerance ◽  
Negative Influence ◽  
Acid Adaptation ◽  
Proteomic Approach ◽  
Serovar Typhimurium ◽  
Tolerance Response ◽  
Porin Proteins

ABSTRACT In Salmonella enterica serovar Typhimurium, the membrane-localized CadC is a transcriptional activator of the cadBA operon, which contributes to the acid tolerance response. Unlike in Escherichia coli, in which transcription of cadC is constitutive, in S. enterica serovar Typhimurium cadC expression is induced by low pH and lysine. Inactivation of cadC suppresses the acid-sensitive phenotype of a cadA mutation, suggesting the existence of other CadC-dependent genes in addition to the cadBA operon. Using a proteomic approach, we identified 8 of the putative CadC-induced proteins and 15 of the putative CadC-repressed proteins. The former include porin proteins OmpC and OmpF. The latter include proteins involved in glycolysis, energy production, and stress tolerance. To better understand the altered levels of OmpC and OmpF, we compared expression of ompR in S. enterica serovar Typhimurium wild-type and cadC mutant strains and determined that CadC exerted a negative influence on ompR transcription. Taken together, our findings strongly suggest that CadC may be a global regulator involved in the OmpR regulatory system during acid adaptation.

scholarly journals Salmonella enterica Serovar Typhimurium and Listeria monocytogenes Acid Tolerance Response Induced by Organic Acids at 20°C: Optimization and Modeling

2003 ◽  
Vol 69 (7) ◽  
pp. 3945-3951 ◽  
Author(s):  
E. J. Greenacre ◽  
T. F. Brocklehurst ◽  
C. R. Waspe ◽  
D. R. Wilson ◽  
P. D. G. Wilson
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Salmonella Enterica ◽  
Acid Tolerance ◽  
Acid Tolerance Response ◽  
Serovar Typhimurium ◽  
Tolerance Response ◽  
Acid Inactivation ◽  
Log Linear

ABSTRACT An acid tolerance response (ATR) has been demonstrated in Listeria monocytogenes and Salmonella enterica serovar Typhimurium in response to low pH poised (i.e., adapted) with acetic or lactic acids at 20°C and modeled by using dynamic differential equations. The ATR was not immediate or prolonged, and optimization occurred after exposure of L. monocytogenes for 3 h at pH 5.5 poised with acetic acid and for 2 h at pH 5.5 poised with lactic acid and after exposure of S. enterica serovar Typhimurium for 2 h at pH 5.5 poised with acetic acid and for 3 h at pH 5.5 poised with lactic acid. An objective mechanistic analysis of the acid inactivation data yielded estimates of the duration of the shoulder (t s ), the log-linear decline (k max), and the magnitude of a critical component (C). The magnitude of k max gave the best agreement with estimates of conditions for optimum ATR induction made from the raw data.

scholarly journals Global Transcriptome and Mutagenic Analyses of the Acid Tolerance Response of Salmonella enterica Serovar Typhimurium

2015 ◽  
Vol 81 (23) ◽  
pp. 8054-8065 ◽  
Author(s):  
Daniel Ryan ◽  
Niladri Bhusan Pati ◽  
Urmesh K. Ojha ◽  
Chandrashekhar Padhi ◽  
Shilpa Ray ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Molecular Mechanisms ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Differentially Expressed ◽  
Acid Tolerance Response ◽  
Content Type ◽  
Serovar Typhimurium ◽  
Tolerance Response

ABSTRACTSalmonella entericaserovar Typhimurium (S. Typhimurium) is one of the leading causative agents of food-borne bacterial gastroenteritis. Swift invasion through the intestinal tract and successful establishment in systemic organs are associated with the adaptability ofS. Typhimurium to different stress environments. Low-pH stress serves as one of the first lines of defense in mammalian hosts, whichS. Typhimurium must efficiently overcome to establish an infection. Therefore, a better understanding of the molecular mechanisms underlying the adaptability ofS. Typhimurium to acid stress is highly relevant. In this study, we have performed a transcriptome analysis ofS. Typhimurium under the acid tolerance response (ATR) and found a large number of genes (∼47%) to be differentially expressed (more than 1.5-fold or less than −1.5-fold;P< 0.01). Functional annotation revealed differentially expressed genes to be associated with regulation, metabolism, transport and binding, pathogenesis, and motility. Additionally, our knockout analysis of a subset of differentially regulated genes facilitated the identification of proteins that contribute toS. Typhimurium ATR and virulence. Mutants lacking genes encoding the K+binding and transport protein KdpA, hypothetical protein YciG, the flagellar hook cap protein FlgD, and the nitrate reductase subunit NarZ were significantly deficient in their ATRs and displayed variedin vitrovirulence characteristics. This study offers greater insight into the transcriptome changes ofS. Typhimurium under the ATR and provides a framework for further research on the subject.

scholarly journals Regulation of metE mRNA expression by FnrS small RNA in Salmonella enterica serovar Typhimurium

2019 ◽  
Vol 229 ◽  
pp. 126319
Author(s):  
Chia-Wei Chien ◽  
Yu-Feng Chan ◽  
Po-Shu Shih ◽  
Jung-En Kuan ◽  
Ke-Feng Wu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Small Rna ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Serovar Typhimurium

Artificial small RNA-mediated growth inhibition in Escherichia coli and Salmonella enterica serovar Typhimurium

2020 ◽  
Vol 521 (3) ◽  
pp. 577-583 ◽  
Author(s):  
Mitsuoki Kawano ◽  
Shota Morohashi ◽  
Kohei Oda ◽  
Masataka Ishikawa ◽  
Shouta Fujita ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Inhibition ◽  
Small Rna ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Serovar Typhimurium

Variability in the adaptive acid tolerance response phenotype of Salmonella enterica strains

2017 ◽  
Vol 62 ◽  
pp. 99-105 ◽  
Author(s):  
Alexandra Lianou ◽  
George-John E. Nychas ◽  
Konstantinos P. Koutsoumanis
Keyword(s):  
Salmonella Enterica ◽  
Acid Tolerance ◽  
Acid Tolerance Response ◽  
Tolerance Response ◽  
Response Phenotype

scholarly journals Novel small RNA (sRNA) landscape of the starvation-stress response transcriptome of Salmonella enterica serovar typhimurium

RNA Biology ◽  
2016 ◽  
Vol 13 (3) ◽  
pp. 331-342 ◽  
Author(s):  
Shivam V. Amin ◽  
Justin T. Roberts ◽  
Dillon G. Patterson ◽  
Alexander B. Coley ◽  
Jonathan A. Allred ◽  
...  
Keyword(s):  
Stress Response ◽  
Small Rna ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Starvation Stress ◽  
Serovar Typhimurium
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