scholarly journals Sister Dehalobacter Genomes Reveal Specialization in Organohalide Respiration and Recent Strain Differentiation Likely Driven by Chlorinated Substrates

2016 ◽  
Vol 7 ◽  
Author(s):  
Shuiquan Tang ◽  
Po Hsiang Wang ◽  
Steven A. Higgins ◽  
Frank E. Löffler ◽  
Elizabeth A. Edwards
2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Yongyi Liang ◽  
Qihong Lu ◽  
Zhiwei Liang ◽  
Xiaokun Liu ◽  
Wenwen Fang ◽  
...  

AbstractObligate and non-obligate organohalide-respiring bacteria (OHRB) play central roles in the geochemical cycling and environmental bioremediation of organohalides. Their coexistence and interactions may provide functional redundancy and community stability to assure organohalide respiration efficiency but, at the same time, complicate isolation and characterization of specific OHRB. Here, we employed a growth rate/yield tradeoff strategy to enrich and isolate a rare non-obligate tetrachloroethene (PCE)-respiring Geobacter from a Dehalococcoides-predominant microcosm, providing experimental evidence for the rate/yield tradeoff theory in population selection. Surprisingly, further physiological and genomic characterizations, together with co-culture experiments, revealed three unique interactions (i.e., free competition, conditional competition and syntrophic cooperation) between Geobacter and Dehalococcoides for their respiration of PCE and polychlorinated biphenyls (PCBs), depending on both the feeding electron donors (acetate/H2 vs. propionate) and electron acceptors (PCE vs. PCBs). This study provides the first insight into substrate-dependent interactions between obligate and non-obligate OHRB, as well as a new strategy to isolate fastidious microorganisms, for better understanding of the geochemical cycling and bioremediation of organohalides.


1987 ◽  
Vol 99 (2) ◽  
pp. 421-428 ◽  
Author(s):  
S. Phongpaichit ◽  
D. W. R. Mackenzie ◽  
C. Fraser

SUMMARYStrains ofCandida albicanscan be differentiated by the morphological features of streak colonies developed on malt agar. A morphotyping system is proposed, where numerical codes are assigned primarily on the basis of the nature and extent of marginal fringing and the surface topography of the streak colony. The system allows ready differentiation to be made of morphotypcs, requires no specialized equipment or expertise and provides a simple and reproducible means for epidemiological studies of candida and candidosis.


Oncology ◽  
1979 ◽  
Vol 36 (6) ◽  
pp. 245-247 ◽  
Author(s):  
H.W. Doerr ◽  
A. Künzler ◽  
H. Schmitz

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 200 ◽  
Author(s):  
Darlene D Wagner ◽  
Laura A Hug ◽  
Janet K Hatt ◽  
Melissa R Spitzmiller ◽  
Elizabeth Padilla-Crespo ◽  
...  

2000 ◽  
Vol 71 (1-2) ◽  
pp. 97-106 ◽  
Author(s):  
C.L. Niblett ◽  
H. Genc ◽  
B. Cevik ◽  
S. Halbert ◽  
L. Brown ◽  
...  

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