scholarly journals Prophylactic Use of Ganoderma lucidum Extract May Inhibit Mycobacterium tuberculosis Replication in a New Mouse Model of Spontaneous Latent Tuberculosis Infection

2016 ◽  
Vol 6 ◽  
Author(s):  
Lingjun Zhan ◽  
Jun Tang ◽  
Shuzhu Lin ◽  
Yanfeng Xu ◽  
Yuhuan Xu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mouse Model ◽  
Ganoderma Lucidum ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Prophylactic Use

scholarly journals Perbedaan Antara Jumlah Sel T Subset Gamma-Delta di Darah Tepi pada Penderita Tuberkulosis dan Orang dengan Latent Tuberculosis Infection

2016 ◽  
Vol 18 (2) ◽  
pp. 162
Author(s):  
Ryzky Widi Atmaja ◽  
Jusak Nugraha
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Gamma Delta

Abstrak Latar Belakang. Imunitas memiliki peranan penting untuk melindungi host dari bacilli Mycobacterium tuberculosis (M.tb), bakteri Obligat  intraseluler  yang  menyebabkan  Tuberkulosis  (TB)  dan  latent  tuberculosis  infection  (LTBI).  Sel  T  subset  gamma-delta (T-γδ) adalah sel-sel potensial tersembunyi yang bermain peran di imunitas innate dan adaptive pada TB. Tetapi, hingga kini perananya   di   LTBI   masih   menjadi   misteri.   Bahan   dan   Metode.   Penelitian   dilakukan   dengan   melibatkan   10 penderita  TB serta 10 orang dengan LTBI. Mereka didapatkan dari Rumah Sakit Paru Surabaya melalui suatu persetujuan kelaikan etik   dari  Universitas  Airlangga.  Sampel-sampel  tersebut  akan  dihitung  jumlah  sel  T-γδ  menggunakan  F A C S C a l i b u r. Hasil.   Jumlah   sel   T-γδ   meningkat   pada   TB   (10,7%)   dan   LTBI   (15, 4%).   Jumlah   dari   kedua   kelompok   tersebut melebihi   rerata   normal   di   darah   tepi   (1% - 5%).   Kesimpulan.   Penigkatan   jumlah   sel   T-γδ   pada   TB   disebabkan melimpahnya kadar IL-12 yang dilepas oleh makrofag selama infeksi. Sementara, peningkatan jumlah sel T-γδ pada LTBI diasumsikan    karena    banyaknya    heat    shock    protein    (HSPs)    yang    dilepas    oleh    M.tb    di    bawah    kondisi    stres. ...Kata  kunci:  tuberkulosis,  latent  tuberculosis  infection,  Mycobacterium  tuberclosis,  sel  T  subset  gamma-d e l t a.

Treatment of latent tuberculosis infection induces changes in multifunctional Mycobacterium tuberculosis-specific CD4+ T cells

2015 ◽  
Vol 205 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Ilaria Sauzullo ◽  
Fabio Mengoni ◽  
Claudia Mascia ◽  
Raffaella Rossi ◽  
Miriam Lichtner ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Cd4 T Cells ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection

scholarly journals Transcription of Genes Involved in Sulfolipid and Polyacyltrehalose Biosynthesis of Mycobacterium tuberculosis in Experimental Latent Tuberculosis Infection

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58378 ◽  
Author(s):  
Jimmy E. Rodríguez ◽  
Ana S. Ramírez ◽  
Laura P. Salas ◽  
Cecilia Helguera-Repetto ◽  
Jorge Gonzalez-y-Merchand ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection

scholarly journals Investigation of the relationship of latent tuberculosis infection with the development of myeloid and lymphoblastic leukemia

2020 ◽  
Vol 24 (1) ◽  
pp. 51-58
Author(s):  
O.P. Lysenko ◽  
I.H. Vlasenko ◽  
V.V. Vlasenko ◽  
O.V. Rymsha ◽  
O.A. Nazarchuk ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Latent Tuberculosis Infection ◽  
Lymphoblastic Leukemia ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Test Material ◽  
Growth Promoter ◽  
Prolonged Incubation ◽  
Antigenic Composition ◽  
The Relationship

Annotation. The relationship between tuberculosis infection and hemoblastosis has attracted the attention of physicians and has been the basis for a wide variety of assumptions. In the second half of the XIX century, there was expressed an opinion about the unique nature of these pathologies. They explained this phenomenon as the immunochemical affinity of specific tumor and mycobacterial antigens, but not as a result of the persistence of mycobacterium tuberculosis (MBT). The aim of the study was to investigate the association of latent tuberculosis infection and oncogenesis, in particular with leukemias. From transplantable human myeloblasts with acute myeloid leukemia (Kasumi-1) and T-lymphocytes with T-lymphoblastic leukemia (Jurkat), including 0.22 μm filtrate of their lysates were isolated from mycobacterium tuberculosis (MBT) with deficient cell wall (CWD). During primary microscopy, the MBT was found mainly in the form of coccoid. The isolated coccoid Kasumi and Jurkat had close antigen affinity and shared antigens with typical MBT. After prolonged incubation with the growth promoter, not only CWD MBT was isolated from all lysates and filtrates, but isolates with identical morphology and antigenic composition were obtained. This strongly suggested that the MBT and their modified forms could be associated with various types of leukemia. Moreover, the affinity of FLK-BLV isolates with blood of people with latent tuberculosis infection, cattle tuberculosis, CWD forms M. bovis and M. tuberculosis was determined, confirming the role of tuberculosis infection in the development of various forms of the disease depending on the host genome, as well as the features of the adaptive reactions of the infectious agent. In particular, after destruction by ultrasound and filtration through Amicon Ultracel® 100 K and Millex® GP 0.22 μm, their filtrates gave an increase in the number of CWD MBT with acid-resistant elements and had the same antigenic composition, including the original isolates. The long-term incubation of the test material in the growth promoter and numerous transplants on the adapted MyCel DW medium played a major role in the allocation of CWD.

scholarly journals Activity of a long-acting injectable bedaquiline formulation in a paucibacillary mouse model of latent tuberculosis infection

2019 ◽  
Author(s):  
Amit Kaushik ◽  
Nicole C. Ammerman ◽  
Sandeep Tyagi ◽  
Vikram Saini ◽  
Iwan Vervoort ◽  
...  
Keyword(s):  
Mouse Model ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Positive Control ◽  
Negative Control ◽  
Tuberculosis Infection ◽  
Sustained Activity ◽  
Short Course ◽  
Long Acting ◽  
Aerosol Infection

ABSTRACTThe potent anti-tuberculosis activity and long half-life of bedaquiline make it an attractive candidate for long-acting/extended release formulations for treatment of latent tuberculosis infection (LTBI). Our objective was to evaluate a long-acting injectable (LAI) bedaquiline formulation in a validated paucibacillary mouse model of LTBI. Following immunization with Mycobacterium bovis rBCG30, BALB/c mice were challenged by aerosol infection with M. tuberculosis H37Rv. Treatment began 13 weeks after challenge infection with one of the following regimens: untreated negative control; positive controls of daily rifampin (10 mg/kg), once-weekly rifapentine (15 mg/kg) and isoniazid (50 mg/kg), or daily bedaquiline (25 mg/kg); test regimens of one, two, or three monthly doses of LAI bedaquiline at 160 mg/dose (BLAI-160); and test regimens of daily bedaquiline at 2.67 (B2.67), 5.33 (B5.33), or 8 (B8) mg/kg to deliver the same total bedaquiline as one, two, or three doses of BLAI-160, respectively. All drugs were administered orally, except for BLAI-160 (intramuscular injection). The primary outcome was the decline in M. tuberculosis lung CFU counts during 12 weeks of treatment. The negative and positive control regimens performed as expected. One, two, and three doses of BLA-160 resulted in decreases of 2.9, 3.2, and 3.5 log10 CFU/lung, respectively by week 12. Daily oral dosing with B2.67, B5.33, and B8 decreased lung CFU counts by 1.6, 2.8, and 4.1 log10, respectively. One dose of BLAI-160 exhibited activity for at least 12 weeks. The sustained activity of BLAI-160 indicates promise as a short-course LTBI treatment requiring few patient encounters to ensure treatment completion.

IN VITRO MODELS OF MYCOBACTERIUM TUBERCULOSIS DORMANCY, AND IN VIVO MODELS OF LATENT TUBERCULOSIS INFECTION

2019 ◽  
Vol 64 (5) ◽  
pp. 299-307
Author(s):  
M. V. Fursov ◽  
I. A. Dyatlov ◽  
V. D. Potapov
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
In Vitro Models ◽  
Tuberculosis Infection ◽  
Published Data ◽  
In Vivo Models ◽  
Dormant State

Modeling of tuberculosis infection is carried out in order to clarify various aspects of the tuberculosis pathogenesis, as well as the testing of new anti-tuberculosis drugs. The characteristic of in vitro models (n = 16) for Mycobacterium tuberculosis dormant state and in vivo models (n = 14) for the latent tuberculosis infection involving several animal species published to date are presented in this review. A brief description of the models and the results obtained by the authors are presented. The analysis of the published data reflects the list of methodological procedures that allow researchers to study the mechanism of the transition of M. tuberculosis cells to a dormant state and reverse to metabolically active state, as well as the process of conversion of active tuberculosis infection to a latent tuberculosis and reactivation.

scholarly journals Paucibacillary Tuberculosis in Mice after Prior Aerosol Immunization with Mycobacterium bovis BCG

2004 ◽  
Vol 72 (2) ◽  
pp. 1065-1071 ◽  
Author(s):  
E. L. Nuermberger ◽  
T. Yoshimatsu ◽  
S. Tyagi ◽  
W. R. Bishai ◽  
J. H. Grosset
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Murine Model ◽  
Low Dose ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Intravenous Route ◽  
Experimental Chemotherapy ◽  
New Treatments

ABSTRACT To develop a murine model of paucibacillary tuberculosis for experimental chemotherapy of latent tuberculosis infection, mice were immunized with viable Mycobacterium bovis BCG by the aerosol or intravenous route and then challenged six weeks later with virulent Mycobacterium tuberculosis. The day after immunization, the counts were 3.71 ± 0.10 log10 CFU in the lungs of aerosol-immunized mice and 3.65 ± 0.11 and 4.93 ± 0.07 log10 CFU in the lungs and spleens of intravenously immunized mice, respectively. Six weeks later, the lungs of all BCG-immunized mice had many gross lung lesions and splenomegaly; the counts were 5.97 ± 0.14 and 3.54 ± 0.07 log10 CFU in the lungs and spleens of aerosol-immunized mice, respectively, and 4.36 ± 0.28 and 5.12 ± 0.23 log10 CFU in the lungs and spleens of intravenously immunized mice, respectively. Mice were then aerosol challenged with M. tuberculosis by implanting 2.37 ± 0.13 log10 CFU in the lungs. Six weeks after challenge, M. tuberculosis had multiplied so that the counts were 6.41 ± 0.27 and 4.44 ± 0.14 log10 CFU in the lungs and spleens of control mice, respectively. Multiplication of M. tuberculosis was greatly limited in BCG-immunized mice. Six weeks after challenge, the counts were 4.76 ± 0.24 and 3.73 ± 0.34 log10 CFU in the lungs of intravenously immunized and aerosol-immunized mice, respectively. In contrast to intravenously immunized mice, there was no detectable dissemination to the spleen in aerosol-immunized mice. Therefore, immunization of mice with BCG by the aerosol route prior to challenge with a low dose of M. tuberculosis resulted in improved containment of infection and a stable paucibacillary infection. This model may prove to be useful for evaluation of new treatments for latent tuberculosis infection in humans.

scholarly journals Gene expression tryptophan aspartate coat protein in determining latent tuberculosis infection using immunocytochemistry and real time polimerase chain reaction

2020 ◽  
Author(s):  
Rebekah J. Setiabudi ◽  
Ni Made Mertaniasih ◽  
Muhammad Amin ◽  
Wayan Tunas Artama
Keyword(s):  
Gene Expression ◽  
Mycobacterium Tuberculosis ◽  
Coat Protein ◽  
Real Time ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Peripheral Blood Monocyte ◽  
Tuberculosis Infection ◽  
Chain Reaction ◽  
The Difference

Background: Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide. Problem of Latent Tuberculosis Infection (LTBI) is increasing in number especially in countries with high TB incidence rate, such as Indonesia. Although not every LTBI will become active TB, if untreated and not handled appropriately it can still be a source of transmission and may increase the rate of resistance to the first-line TB drugs. Mycobacterium tuberculosis as a cause of tuberculosis disease is an intracellular pathogens that survives within the phagosome of host macrophages. Several host factors are involved in this process, including the Tryptophan Aspartate-containing Coat Protein (TACO). TACO is a protein recruited and retained by viable Mycobacterium tuberculosis on the surface of the phagosome membrane to maintain its survival in phagosome, because the presence of TACO plays an important role in inhibiting the fusion of phagosomes and lysosomes. Objective: the aim of this studyis to assess the difference of gene expression TACO protein in Latent Tuberculosis Infection (LTBI) and healthy people. Method: A preliminary studyof mRNA examination of TACO protein using Immunocytochemistry (ICC) and Real Time-Polimerase Chain Reaction (RT-PCR) method by a PCR Light Cycler 2.0 machine (Roche) in LTBI and healthy groups. Results: 18 samples of peripheral blood monocyte cells (PBMCs) were collected and divided into 2 groups. We found that there was a significantly difference between the 2 groups of samples. Conclusion: Further research is required to consider that the measurement of TACO expression using RT-PCRcan used as one of the other method to determine LTBI.

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