scholarly journals Probing an Interfacial Surface in the Cyanide Dihydratase from Bacillus pumilus, A Spiral Forming Nitrilase

2016 ◽  
Vol 6 ◽  
Author(s):  
Jason M. Park ◽  
Andani Mulelu ◽  
B. Trevor Sewell ◽  
Michael J. Benedik
Keyword(s):  
Bacillus Pumilus ◽  
Interfacial Surface ◽  
Cyanide Dihydratase

scholarly journals Isolation and characterization of a cyanide dihydratase from Bacillus pumilus C1.

1993 ◽  
Vol 175 (19) ◽  
pp. 6105-6112 ◽  
Author(s):  
P R Meyers ◽  
D E Rawlings ◽  
D R Woods ◽  
G G Lindsey
Keyword(s):  
Bacillus Pumilus ◽  
Isolation And Characterization ◽  
Cyanide Dihydratase

scholarly journals CynD, the Cyanide Dihydratase from Bacillus pumilus: Gene Cloning and Structural Studies

2003 ◽  
Vol 69 (8) ◽  
pp. 4794-4805 ◽  
Author(s):  
Dakshina Jandhyala ◽  
Mark Berman ◽  
Paul R. Meyers ◽  
B. Trevor Sewell ◽  
Richard C. Willson ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Spiral Structure ◽  
Sequence Similarity ◽  
Affinity Purification ◽  
Three Dimensional ◽  
Pseudomonas Stutzeri ◽  
Recombinant Enzyme ◽  
Dimensional Reconstruction ◽  
Short Spiral ◽  
Cyanide Dihydratase

ABSTRACT The cyanide dihydratase in Bacillus pumilus was shown to be an 18-subunit spiral structure by three-dimensional reconstruction of electron micrographs of negatively stained material at its optimum pH, 8.0. At pH 5.4, the subunits rearrange to form an extended left-handed helix. Gel electrophoresis of glutaraldehyde cross-linked enzyme suggests that the fundamental component of the spiral is a dimer of the 37-kDa subunit. The gene was cloned, and the recombinant enzyme was readily expressed at high levels in Escherichia coli. Purification of the recombinant enzyme was facilitated by the addition of a C-terminal six-histidine affinity purification tag. The tagged recombinant enzyme has Km and V max values similar to those published for the native enzyme. This is the first cyanide dihydratase from a gram-positive bacterium to be sequenced, and it is the first description of the structure of any member of this enzyme class. The putative amino acid sequence shares over 80% identity to the only other sequenced cyanide dihydratase, that of the gram-negative Pseudomonas stutzeri strain AK61, and is similar to a number of other bacterial and fungal nitrilases. This sequence similarity suggests that the novel short spiral structure may be typical of these enzymes. In addition, an active cyanide dihydratase from a non-cyanide-degrading isolate of B. pumilus (strain 8A3) was cloned and expressed. This suggests that cynD, the gene coding for the cyanide dihydratase, is not unique to the C1 strain of B. pumilus and is not a reflection of its origin at a mining waste site.

scholarly journals The structure of the cyanide dihydratase (CynD) from Bacillus pumilus

2017 ◽  
Vol 73 (a2) ◽  
pp. C1296-C1296
Author(s):  
Bryan Trevor Sewell ◽  
Achilleas Frangakis ◽  
Andani Mulelu ◽  
Julian Reitz
Keyword(s):  
Bacillus Pumilus ◽  
Cyanide Dihydratase

Residue Y70 of the Nitrilase Cyanide Dihydratase from Bacillus pumilus Is Critical for Formation and Activity of the Spiral Oligomer

2016 ◽  
Vol 26 (12) ◽  
pp. 2179-2183
Author(s):  
Jason M. Park ◽  
Christian M. Ponder ◽  
B. Trevor Sewell ◽  
Michael J. Benedik
Keyword(s):  
Bacillus Pumilus ◽  
Cyanide Dihydratase

Exemplar Abstract for Bacillus pumilus Meyer and Gottheil 1901 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Bacillus Pumilus

Nomenclature Abstract for Bacillus pumilus Meyer and Gottheil 1901 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Bacillus Pumilus

ELECTROHYDRODYNAMIC INSTABILITY OF LIQUID SHEET SATURATING POROUS MEDIUM WITH INTERFACIAL SURFACE CHARGES

2013 ◽  
Vol 23 (2) ◽  
pp. 165-191
Author(s):  
Mohamed F. El-Sayed ◽  
M. H. M. Moussa ◽  
Ahmed A. A. Hassan ◽  
N. M. Hafez
Keyword(s):  
Porous Medium ◽  
Liquid Sheet ◽  
Surface Charges ◽  
Electrohydrodynamic Instability ◽  
Interfacial Surface

STUDY ON ALGICIDAL EFFECTS OF BACILLUS PUMILUS

2013 ◽  
Vol 36 (6) ◽  
pp. 1184-1187
Author(s):  
Ming-Ming HU ◽  
Jing DONG ◽  
Yan-Hui LI ◽  
Yin-Wu SHEN ◽  
Yong-Ding LIU ◽  
...  
Keyword(s):  
Bacillus Pumilus ◽  
Algicidal Effects
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