scholarly journals Single-Cell Analysis of Growth and Cell Division of the Anaerobe Desulfovibrio vulgaris Hildenborough

2015 ◽  
Vol 6 ◽  
Author(s):  
Anouchka Fievet ◽  
Adrien Ducret ◽  
Tâm Mignot ◽  
Odile Valette ◽  
Lydia Robert ◽  
...  
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Desulfovibrio Vulgaris ◽  
Cell Analysis ◽  
Desulfovibrio Vulgaris Hildenborough

scholarly journals Balanced transcription of cell division genes inBacillus subtilisas revealed by single cell analysis

2013 ◽  
Vol 15 (12) ◽  
pp. 3196-3209 ◽  
Author(s):  
Erik Nico Trip ◽  
Jan-Willem Veening ◽  
Eric J. Stewart ◽  
Jeff Errington ◽  
Dirk-Jan Scheffers
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

scholarly journals Dinoroseobacter shibae outer membrane vesicles are enriched for the chromosome dimer resolution site dif

2019 ◽  
Author(s):  
Hui Wang ◽  
Nicole Beier ◽  
Christian Bödeker ◽  
Helena Sztajer ◽  
Petra Henke ◽  
...  
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Outer Membrane ◽  
Single Cell Analysis ◽  
Saturated Fatty Acids ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Cell Analysis ◽  
Septum Formation ◽  
Dinoroseobacter Shibae

AbstractOuter membrane vesicles (OMVs) of Gram-negative bacteria have key roles in pathogenesis. However, little is known about their biogenesis and cargo in marine bacteria. In Dinoroseobacter shibae, a marine member of the Rhodobacteraceae, OMVs were produced throughout exponential growth, and DNA could be detected by fluorescence microscopy inside appr. 65% of vesicles. Single cell analysis using time-lapse microscopy showed that individual cells secreted multiple OMVs, preferentially at the septum during cell division. OMVs were enriched for saturated fatty acids, thus their secretion likely increases the fluidity of the membrane of the releasing cell locally. DNA was isolated from the vesicle lumen and sequenced; it was up to 40fold enriched for the region around the terminus of replication (ter). Within this region, the peak of coverage of vesicle DNA was located at dif, a conserved 28 bp palindromic sequence required for binding of the site specific tyrosine recombinases XerCD which are activated by the divisome protein FtsK immediately prior to septum formation. Some of the most abundant proteins of the vesicle proteome were predicted to be required for direct interaction with peptidoglycan during cell division. Single cell analysis, electron microscopy, proteome and DNA cargo show that constitutive OMV secretion in D. shibae occurs mainly prior to septum formation. The footprint of the FtsK/XerCD molecular machinery which resolves chromosome dimers suggests a novel highly conserved route for incorporation of DNA into OMVs. Clearing the division site from small DNA fragments might be an important function of this type of vesicles.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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