scholarly journals The inactivation of RNase G reduces the Stenotrophomonas maltophilia susceptibility to quinolones by triggering the heat shock response

2015 ◽  
Vol 6 ◽  
Author(s):  
Alejandra Bernardini ◽  
Fernando Corona ◽  
Ricardo Dias ◽  
Maria B. Sánchez ◽  
Jose L. Martínez
Keyword(s):  
Heat Shock ◽  
Heat Shock Response ◽  
Stenotrophomonas Maltophilia ◽  
Shock Response ◽  
Rnase G

Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish

2018 ◽  
Vol 69 (2) ◽  
pp. 337-340
Author(s):  
Vlad Preluca ◽  
Bogdan Horatiu Serb ◽  
Sanda Marchian ◽  
Diter Atasie ◽  
Mihaela Cernusca Mitariu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Dimethyl Sulfoxide ◽  
Heat Shock Response ◽  
Protein Misfolding ◽  
Fluorescent Microscopy ◽  
Screening Tests ◽  
Transgenic Zebrafish ◽  
Fluorescent Intensity ◽  
Shock Response ◽  
Heat Shock Induction

Heat shock inductors have potential as treatment for degenerative and protein misfolding diseases. Dimethyl-sulfoxide is widely used as a solvent in pharmacological screening tests and has been shown to have heat shock induction effects. Transgenic Tg (hsp70l:EGFP-HRAS_G12V)io3(AB) zebrafish larvae were exposed for 24 hours to dimethyl-sulfoxide in concentratios of 0.1-2%, and to moderate heat shock inductors pentoxifylline and tacrolimus. Positive controls were exposed to 35, 38 and 40�C for 20 min, and incubated for 24 h at 28�C. Heat shock response was measured by fluorescence microscopy and signal intensity quantification in FIJI. Dimethyl-sulfoxide caused a dose-dependant increase in fluorescent intensity, but significantly lower compared with exposure to 38 and 40�C. Pentoxifylline and tacrolimus induced a significantly higher increase in fluorescence compared with 0.5% dimethyl-sulfoxide. Thus, although dimethyl-sulfoxide has independent heat shock induction effects, concentrations of up to 0.5% are suitable for heat shock response screening tests.

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