scholarly journals Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2

2015 ◽  
Vol 6 ◽  
Author(s):  
Xiaoke Hu ◽  
Caixia Wang ◽  
Peng Wang
Keyword(s):  
Biosurfactant Production ◽  
Marine Vibrio ◽  
Marine Vibrio Sp

scholarly journals Purification and Characterization of Alginate Lyase from Marine Vibrio sp. YWA

2006 ◽  
Vol 38 (9) ◽  
pp. 633-638 ◽  
Author(s):  
Yuan-Hong WANG ◽  
Guang-Li YU ◽  
Xin-Min WANG ◽  
Zhi-Hua LV ◽  
Xia ZHAO ◽  
...  
Keyword(s):  
Alginate Lyase ◽  
Purification And Characterization ◽  
Marine Vibrio ◽  
Marine Vibrio Sp

Aerobactin production by a planktonic marine Vibrio sp

1993 ◽  
Vol 38 (5) ◽  
pp. 1091-1097 ◽  
Author(s):  
Margo G. Haygood ◽  
Pamela D. Holt ◽  
Alison Butler
Keyword(s):  
Marine Vibrio ◽  
Marine Vibrio Sp

Extracellular nuclease produced by a marine bacterium. II. Purification and properties of extracellular nuclease from a marine Vibrio sp.

1976 ◽  
Vol 22 (10) ◽  
pp. 1443-1452 ◽  
Author(s):  
M. Maeda ◽  
N. Taga
Keyword(s):  
Enzyme Activity ◽  
Deae Cellulose ◽  
Strong Stability ◽  
Rnase Activity ◽  
Salting Out ◽  
Deae Cellulose Column ◽  
Marine Vibrio ◽  
Extracellular Nuclease ◽  
Marine Vibrio Sp ◽  
Cellulose Column

Extracellular nuclease produced by a marine Vibrio sp., strain No. 2, was purified by salting out with ammonium sulfate and by chromatography on a DEAE-cellulose column and twice on a Sephadex G-200 column. The nuclease was eluted as a single peak in which the deoxyribonuclease (DNase) activity and ribonuclease (RNase) activity appeared together. Polyacrylamide disc gel electrophoresis showed a single band of stained protein which had both DNase and RNase activity. The molecular weight of the enzyme was estimated to be 100 000 daltons. When using partially purified enzyme from the DEAE-cellulose column, the optimum pH for activity was 8.0, and the enzyme was activated strongly by 0.05 M Mg2+ ion and stabilized by 0.01 M Ca2+ ion. These concentrations of Mg2+ and Ca2+ ions are similar to those of the two cations in seawater. Indeed, the enzyme revealed high activity and strong stability when kept in seawater. The presence of particulate matter, such as cellulose powder, chitin powder, Hyflosupercel, Kaolin, and marine mud increased the stability of the enzyme. When the hydrostatic pressure was increased from 1 to 1000 atmospheres, the decrements of the enzyme activity were more pronounced at 30 and 40 °C than at 25 or 50 °C. The enzyme activity was restored after decompression to 1 atm at 30 °C.

Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp. QY102

2013 ◽  
Vol 37 (3) ◽  
pp. 575-584 ◽  
Author(s):  
Jiushun Zhou ◽  
Menghao Cai ◽  
Tao Jiang ◽  
Weiqiang Zhou ◽  
Wei Shen ◽  
...  
Keyword(s):  
Carbon Source ◽  
Control Strategy ◽  
Alginate Lyase ◽  
Source Control ◽  
Mixed Carbon Source ◽  
Marine Vibrio ◽  
Marine Vibrio Sp

Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant / Topraktan izole edilen bakterilerden biyosurfaktan üretiminin optimizasyonu ve surfaktanın karakterizasyonu

2016 ◽  
Vol 41 (5) ◽  
Author(s):  
Yağmur Toptaş ◽  
Meltem Çelikdemir ◽  
Cansel Tuncer ◽  
Yeliz Buruk Şahin ◽  
Pınar Aytar Çelik ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Contaminated Soil ◽  
Biosurfactant Production ◽  
Production Medium ◽  
Accession Number ◽  
Surface Active ◽  
Statistical Screening ◽  
Active Substances ◽  
Significant Factors

AbstractObjective: Biosurfactants are surface-active substances produced by microorganisms. The growth of microorganism supports biosurfactant production on hydrocarbon polluted environments. In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon- contaminated soil was studied.Methods: The components of biosurfactant production medium were investigated through Plackett-Burman methodology. By using Placket-Burman based on statistical screening, six of the eleven factors of production medium were found to be critically effective on the biosurfactant production.Results: The significant factors were KCl, ZnSOConclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.

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