scholarly journals Molecular characterization of a bipartite double-stranded RNA virus and its satellite-like RNA co-infecting the phytopathogenic fungus Sclerotinia sclerotiorum

2015 ◽  
Vol 6 ◽  
Author(s):  
Lijiang Liu ◽  
Qihua Wang ◽  
Jiasen Cheng ◽  
Yanping Fu ◽  
Daohong Jiang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sclerotinia Sclerotiorum ◽  
Rna Virus ◽  
Phytopathogenic Fungus ◽  
Double Stranded Rna

Identification and molecular characterization of a new nonsegmented double-stranded RNA virus isolated from Culex mosquitoes in Japan

2011 ◽  
Vol 155 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Haruhiko Isawa ◽  
Ryusei Kuwata ◽  
Keita Hoshino ◽  
Yoshio Tsuda ◽  
Kouji Sakai ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rna Virus ◽  
Double Stranded Rna ◽  
Culex Mosquitoes

Identification and molecular characterization of a new double-stranded RNA virus infectingChondrostereum purpureum

2008 ◽  
Vol 30 (4) ◽  
pp. 604-613 ◽  
Author(s):  
S.F. Shamoun ◽  
A. Varga ◽  
R.A. Valverde ◽  
T. Ramsfield ◽  
G. Sumampong ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rna Virus ◽  
Double Stranded Rna

Characterization of a novel Sclerotinia sclerotiorum RNA virus as the prototype of a new proposed family within the order Tymovirales

2016 ◽  
Vol 219 ◽  
pp. 92-99 ◽  
Author(s):  
Kunfei Li ◽  
Dan Zheng ◽  
Jiasen Cheng ◽  
Tao Chen ◽  
Yanping Fu ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Rna Virus

scholarly journals Molecular characterization of an unusual new plant RNA virus reveals an evolutionary link between two different virus families

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206382 ◽  
Author(s):  
Sun-Jung Kwon ◽  
Gug-Seoun Choi ◽  
Boram Choi ◽  
Jang-Kyun Seo
Keyword(s):  
Molecular Characterization ◽  
Rna Virus

Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea

2016 ◽  
Vol 162 (1) ◽  
pp. 313-316 ◽  
Author(s):  
Fangmin Hao ◽  
Ziliang Zhou ◽  
Mingde Wu ◽  
Guoqing Li
Keyword(s):  
Botrytis Cinerea ◽  
Molecular Characterization ◽  
Phytopathogenic Fungus

scholarly journals Cloning and molecular characterization of a new fungal xylanase gene from Sclerotinia sclerotiorum S2

BMB Reports ◽  
2011 ◽  
Vol 44 (10) ◽  
pp. 653-658 ◽  
Author(s):  
Olfa Elleuch Ellouze ◽  
Sana Loukil ◽  
Mohamed Nejib Marzouki
Keyword(s):  
Molecular Characterization ◽  
Sclerotinia Sclerotiorum ◽  
Xylanase Gene

scholarly journals Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258318
Author(s):  
Marta Antas ◽  
Monika Olech ◽  
Anna Szczotka-Bochniarz
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rna Virus ◽  
Economic Losses ◽  
High Morbidity ◽  
Antigenic Analysis ◽  
S Gene ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus

Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.

scholarly journals Genome organization and molecular characterization of the three Formica exsecta viruses—FeV1, FeV2 and FeV4

PeerJ ◽  
2019 ◽  
Vol 6 ◽  
pp. e6216 ◽  
Author(s):  
Kishor Dhaygude ◽  
Helena Johansson ◽  
Jonna Kulmuni ◽  
Liselotte Sundström
Keyword(s):  
Molecular Characterization ◽  
Genome Organization ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Link Type ◽  
Infection Pathways ◽  
Comparative Genomics Analysis ◽  
First Time ◽  
Negative Sense

We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

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