Regulatory role of tetR gene in a novel gene cluster of Acidovorax avenae subsp. avenae RS-1 under oxidative stress

Faculty Opinions recommendation of Expression analysis of the tylosin-biosynthetic gene cluster: pivotal regulatory role of the tylQ product.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004877.55457 ◽

2002 ◽

Author(s):

Ben Shen

Keyword(s):

Gene Cluster ◽

Expression Analysis ◽

Biosynthetic Gene Cluster ◽

Regulatory Role ◽

Biosynthetic Gene

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Abstract 125: Perturbation of Mitochondrial Calcium Uniporter Promotes Cardiac Oxidative Stress and Autophagy During Heart Failure

Circulation Research ◽

10.1161/res.121.suppl_1.125 ◽

2017 ◽

Vol 121 (suppl_1) ◽

Author(s):

Sudarsan Rajan ◽

Santhanam Shanmughapriya ◽

Dhanendra Tomar ◽

Zhiwei Dong ◽

Joseph Y Cheung ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Mouse Model ◽

Atp Depletion ◽

Regulatory Role ◽

Mitochondrial Calcium ◽

Mitochondrial Bioenergetics ◽

Complex Assembly ◽

New Findings

Mitochondrial calcium ([Ca 2+ ] m ) is essential for cardiomyocyte viability, and aberration of [Ca 2+ ] m is known to elicit multiple cardiac stress conditions associated with ATP depletion, reactive oxygen species, and mitochondrial permeability transition pore opening, all of which can lead to metabolic stress and the loss of dysfunctional mitochondria by aberrant autophagy. Elucidating the regulatory role of m itochondrial c alcium u niporter (MCU)-mediated [Ca 2+ ] m in modulating cardiac mitochondrial bioenergetics and autophagy has high significance and clinical impact for many pathophysiological processes. [Ca 2+ ] m is exquisitely controlled by the inner mitochondrial membrane uniporter, transporters, regulators and exchangers including MCU, MCUR1, EMRE, MICU1, MICU2 and LETM1. Our recently published findings revealed that Mitochondrial Ca 2+ Uniporter Regulator 1 (MCUR1) serves as a scaffold factor for uniporter complex assembly. We found that deletion of MCUR1 impaired [Ca 2+ ] m uptake, mitochondrial Ca 2+ current ( I MCU ) and mitochondrial bioenergetics and is associated with increased autophagy. Our new findings indicate that the impairment of [Ca 2+ ] m uptake exacerbated autophagy following ischemia-reperfusion (I/R) injury. In support of our mouse model, human failing hearts show that MCUR1 protein levels are markedly decreased and autophagy markers are increased, demonstrating a crucial link between [Ca 2+ ] m uptake and autophagy during heart failure. Additionally, our results reveal that either oxidation or disruption of human MCU Cys-97 (in mouse Cys-96; gain-of-function MCU C96A mutant) produces a conformational change within the N terminal β-grasp fold of MCU which promotes higher-order MCU complex assembly and increased I MCU activity and mitochondrial ROS levels. The results of our studies using a novel cardiac-specific MCUR1-KO model and a constitutively active global MCU C96A KI mouse model (CRISPR-Cas9 genome edited) elucidate the regulatory role of [Ca 2+ ] m in cardiac bioenergetics and autophagy during oxidative stress and myocardial infarction. Thus, targeting assembly and the activity of MCU complex will offer a new potential therapeutic target in the treatment of cardiomyopathy and heart failure.

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The Regulatory Role of MiR-203 in Oxidative Stress induced Cell Injury Through the CBS/H2S Pathway

Nitric Oxide ◽

10.1016/j.niox.2021.10.007 ◽

2021 ◽

Author(s):

Qiuyan Zhang ◽

Zhuqing Shen ◽

Yaqi Shen ◽

Muye Ma ◽

Hao Jue ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Injury ◽

Regulatory Role

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IdeR, a DtxR Family Iron Response Regulator, Controls Iron Homeostasis, Morphological Differentiation, Secondary Metabolism, and the Oxidative Stress Response inStreptomyces avermitilis

Applied and Environmental Microbiology ◽

10.1128/aem.01503-18 ◽

2018 ◽

Vol 84 (22) ◽

Cited By ~ 5

Author(s):

Yaqing Cheng ◽

Renjun Yang ◽

Mengya Lyu ◽

Shiwei Wang ◽

Xingchao Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Secondary Metabolism ◽

Iron Homeostasis ◽

Morphological Differentiation ◽

Oxidative Stress Response ◽

Regulatory Role ◽

Intracellular Iron ◽

Content Type

ABSTRACTIron, an essential element for microorganisms, functions as a vital cofactor in a wide variety of key metabolic processes. On the other hand, excess iron may have toxic effects on bacteria by catalyzing the formation of reactive oxygen species through the Fenton reaction. The prevention of iron toxicity requires the precise control of intracellular iron levels in bacteria. Mechanisms of iron homeostasis in the genusStreptomyces(the producers of various antibiotics) are poorly understood.Streptomyces avermitilisis the industrial producer of avermectins, which are potent anthelmintic agents widely used in medicine, agriculture, and animal husbandry. We investigated the regulatory role of IdeR, a DtxR family regulator, inS. avermitilis. In the presence of iron, IdeR binds to a specific palindromic consensus sequence in promoters and regulates 14 targets involved in iron metabolism (e.g., iron acquisition, iron storage, heme metabolism, and Fe-S assembly). IdeR also directly regulates 12 targets involved in other biological processes, including morphological differentiation, secondary metabolism, carbohydrate metabolism, and the tricarboxylic acid (TCA) cycle.ideRtranscription is positively regulated by the peroxide-sensing transcriptional regulator OxyR. A newly constructedideRdeletion mutant (DideR) was found to be less responsive to iron levels and more sensitive to H2O2treatment than the wild-type strain, indicating thatideRis essential for oxidative stress responses. Our findings, taken together, demonstrate that IdeR plays a pleiotropic role in the overall coordination of metabolism inStreptomycesspp. in response to iron levels.IMPORTANCEIron is essential to almost all organisms, but in the presence of oxygen, iron is both poorly available and potentially toxic.Streptomycesspecies are predominantly present in soil where the environment is complex and fluctuating. So far, the mechanism of iron homeostasis inStreptomycesspp. remains to be elucidated. Here, we characterized the regulatory role of IdeR in the avermectin-producing organismS. avermitilis. IdeR maintains intracellular iron levels by regulating genes involved in iron absorption and storage. IdeR also directly regulates morphological differentiation, secondary metabolism, and central metabolism.ideRis under the positive control of OxyR and is indispensable for an efficient response to oxidative stress. This investigation uncovered that IdeR acts as a global regulator coordinating iron homeostasis, morphological differentiation, secondary metabolism, and oxidative stress response inStreptomycesspecies. Elucidation of the pleiotropic regulation function of IdeR provides new insights into the mechanisms of howStreptomycesspp. adapt to the complex environment.

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Regulatory role of mitochondria in oxidative stress and atherosclerosis

World Journal of Cardiology ◽

10.4330/wjc.v2.i6.150 ◽

2010 ◽

Vol 2 (6) ◽

pp. 150 ◽

Cited By ~ 42

Author(s):

Jui-Chih Chang

Keyword(s):

Oxidative Stress ◽

Regulatory Role

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Drosophila suzukii oxidative stress response involves Jheh gene cluster but not transposable elements

10.1101/2020.04.27.063297 ◽

2020 ◽

Author(s):

Pierre Marin ◽

Angelo Jacquet ◽

Hélène Henri ◽

Patricia Gibert ◽

Cristina Vieira

Keyword(s):

Oxidative Stress ◽

Invasive Species ◽

Transposable Elements ◽

Gene Cluster ◽

Invasive Pest ◽

Global Changes ◽

Drosophila Suzukii ◽

Invasive Status ◽

Summary Statement

ABSTRACTThe study of the mechanisms involved in adaptation remains a timely issue, particularly in the context of global changes. To better understand these mechanisms of rapid adaptation, invasive species are a good model because they are subjected to new and/or different environmental factors. Using different lines of different geographical origin of the invasive pest Drosophila suzukii, we characterized the phenotypic response to oxidative stress. Subsequently, we tested the involvement of the Jheh gene cluster in this response and the possible role of transposable elements. We show that the resistance to oxidative stress of the lines appears to be related to their invasive status and we confirm the role of the Jheh gene cluster in this response. We have not identified any transposable elements in this gene region that could influence the expression of the gene.Summary statementThe responses to oxidative stress of the invasive species, Drosophila suzukii, show variability between genotypes related to their invasion status. The genes of the juvenile hormone epoxide hydrolase cluster are involved in this response.

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