scholarly journals Diversity of reductive dehalogenase genes from environmental samples and enrichment cultures identified with degenerate primer PCR screens

2013 ◽  
Vol 4 ◽  
Author(s):  
Laura A. Hug ◽  
Elizabeth A. Edwards
Keyword(s):  
Environmental Samples ◽  
Degenerate Primer ◽  
Reductive Dehalogenase ◽  
Enrichment Cultures ◽  
Reductive Dehalogenase Genes

scholarly journals Extrachromosomal circular elements targeted by CRISPR-Cas in Dehalococcoides mccartyi are linked to mobilization of reductive dehalogenase genes

2018 ◽  
Vol 13 (1) ◽  
pp. 24-38 ◽  
Author(s):  
Olivia Molenda ◽  
Shuiquan Tang ◽  
Line Lomheim ◽  
Vasu K. Gautam ◽  
Sofia Lemak ◽  
...  
Keyword(s):  
Reductive Dehalogenase ◽  
Dehalococcoides Mccartyi ◽  
Reductive Dehalogenase Genes

scholarly journals Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases

2013 ◽  
Vol 368 (1616) ◽  
pp. 20120322 ◽  
Author(s):  
Laura A. Hug ◽  
Farai Maphosa ◽  
David Leys ◽  
Frank E. Löffler ◽  
Hauke Smidt ◽  
...  
Keyword(s):  
Classification System ◽  
Sequence Similarity ◽  
Halogenated Hydrocarbons ◽  
Sequence Information ◽  
Sequencing Data ◽  
Functional Prediction ◽  
Groundwater Contaminants ◽  
Reductive Dehalogenase ◽  
Bacterial Phyla ◽  
Reductive Dehalogenase Genes

Organohalide respiration is an anaerobic bacterial respiratory process that uses halogenated hydrocarbons as terminal electron acceptors during electron transport-based energy conservation. This dechlorination process has triggered considerable interest for detoxification of anthropogenic groundwater contaminants. Organohalide-respiring bacteria have been identified from multiple bacterial phyla, and can be categorized as obligate and non-obligate organohalide respirers. The majority of the currently known organohalide-respiring bacteria carry multiple reductive dehalogenase genes. Analysis of a curated set of reductive dehalogenases reveals that sequence similarity and substrate specificity are generally not correlated, making functional prediction from sequence information difficult. In this article, an orthologue-based classification system for the reductive dehalogenases is proposed to aid integration of new sequencing data and to unify terminology.

Development and application of a rapid, user-friendly, and inexpensive method to detect Dehalococcoides sp. reductive dehalogenase genes from groundwater

2017 ◽  
Vol 101 (11) ◽  
pp. 4827-4835 ◽  
Author(s):  
Yogendra H. Kanitkar ◽  
Robert D. Stedtfeld ◽  
Paul B. Hatzinger ◽  
Syed A. Hashsham ◽  
Alison M. Cupples
Keyword(s):  
Inexpensive Method ◽  
Reductive Dehalogenase ◽  
Reductive Dehalogenase Genes ◽  
User Friendly

scholarly journals Comparative analysis of metagenomes from three methanogenic hydrocarbon-degrading enrichment cultures with 41 environmental samples

2015 ◽  
Vol 9 (9) ◽  
pp. 2028-2045 ◽  
Author(s):  
Boonfei Tan ◽  
S Jane Fowler ◽  
Nidal Abu Laban ◽  
Xiaoli Dong ◽  
Christoph W Sensen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Environmental Samples ◽  
Enrichment Cultures

scholarly journals Grape pomace compost harbors organohalide-respiring Dehalogenimonas species with novel reductive dehalogenase genes

2017 ◽  
Vol 11 (12) ◽  
pp. 2767-2780 ◽  
Author(s):  
Yi Yang ◽  
Steven A Higgins ◽  
Jun Yan ◽  
Burcu Şimşir ◽  
Karuna Chourey ◽  
...  
Keyword(s):  
Grape Pomace ◽  
Reductive Dehalogenase ◽  
Reductive Dehalogenase Genes

scholarly journals Complete Genome Sequence of Dehalococcoides mccartyi Strain FL2, a Trichloroethene-Respiring Anaerobe Isolated from Pristine Freshwater Sediment

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Jun Yan ◽  
Yi Yang ◽  
Xiuying Li ◽  
Frank E. Löffler
Keyword(s):  
Complete Genome Sequence ◽  
Reductive Dechlorination ◽  
Complete Genome ◽  
Hydrogen Oxidation ◽  
Protein Coding ◽  
Coding Sequences ◽  
Content Type ◽  
Reductive Dehalogenase ◽  
Dehalococcoides Mccartyi ◽  
Reductive Dehalogenase Genes

Dehalococcoides mccartyi strain FL2 couples growth to hydrogen oxidation and reductive dechlorination of trichloroethene and cis- and trans-1,2-dichloroethenes. Strain FL2 has a 1.42-Mb genome with a G+C content of 47.0% and carries 1,465 protein-coding sequences, including 24 reductive dehalogenase genes.

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