scholarly journals Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase

2012 ◽  
Vol 3 ◽  
Author(s):  
Victoria R. Nachar ◽  
Francisco C. Savka ◽  
Sean E. McGroty ◽  
Katherine A. Donovan ◽  
Rachel A. North ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Lysine Biosynthesis ◽  
Partial Characterization ◽  
Biosynthesis Pathway ◽  
Verrucomicrobium Spinosum

scholarly journals Biochemical analysis of the methylic antigen of Paracoccidioides brasiliensis

1992 ◽  
Vol 34 (6) ◽  
pp. 511-516 ◽  
Author(s):  
Júnia Soares Hamdan ◽  
Maria Aparecida de Resende ◽  
Sarah Piancastelli Franzot ◽  
Eduardo Osório Cisalpino
Keyword(s):  
Total Lipid ◽  
Biochemical Composition ◽  
Biochemical Analysis ◽  
Paracoccidioides Brasiliensis ◽  
Synthetic Medium ◽  
The Other ◽  
Partial Characterization ◽  
Biochemical Characteristics ◽  
Carbohydrate Contents

Yeast forms of five strains of Paracoccidioides brasiliensis (SN, 2, 18, 192 and JT- 1) were cultured in a synthetic medium for obtaining methylic antigens. These antigens were lyophilized and studied for each strain, to determine their partial biochemical composition, through measurements of total lipid, protein and carbohydrate contents. Lipids of methylic antigens were purified and analysed for sterols, phospholipids, glycolipids, li-poproteins, and partial characterization of sterols. Significant differences were found among antigenic preparations derived from distinct P. brasiliensis strains, in relation to the quantitative determinations. On the other hand, sterol analysis revealed the presence of ergosterol, lanosterol and squalene in all samples. The diversity verified in the biochemical characteristics of antigens derived from different P. brasiliensis strains, confirm the need of using a pool of fungal samples in order to produce antigen preparations for serological procedures without hampering their sensitivity.

scholarly journals Biochemical and Phylogenetic Characterization of a Novel Diaminopimelate Biosynthesis Pathway in Prokaryotes Identifies a Diverged Form of ll-Diaminopimelate Aminotransferase

2008 ◽  
Vol 190 (9) ◽  
pp. 3256-3263 ◽  
Author(s):  
André O. Hudson ◽  
Charles Gilvarg ◽  
Thomas Leustek
Keyword(s):  
Genomic Analysis ◽  
Direct Conversion ◽  
Amino Acid Identity ◽  
Comparative Genomic Analysis ◽  
Lysine Biosynthesis ◽  
Comparative Genomic ◽  
Biosynthesis Pathway ◽  
Acid Identity ◽  
Phylogenetic Characterization

ABSTRACT A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an ll-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of l-2,3,4,5-tetrahydrodipicolinate to ll-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged forms of DapL (DapL1 and DapL2). DapL orthologs were identified in eubacteria and archaea. In some species the corresponding dapL gene was found to lie in genomic contiguity with other dap genes, suggestive of a polycistronic structure. The DapL candidate enzymes were found to cluster into two classes sharing approximately 30% amino acid identity. The function of selected enzymes from each class was studied. Both classes were able to functionally complement Escherichia coli dapD and dapE mutants and to catalyze ll-DAP transamination, providing functional evidence for a role in DAP/lysine biosynthesis. In all cases the occurrence of dapL in a species correlated with the absence of genes for dapD and dapE representing the acyl DAP pathway variants, and only in a few cases was dapL coincident with ddh encoding meso-DAP dehydrogenase. The results indicate that the DapL pathway is restricted to specific lineages of eubacteria including the Cyanobacteria, Desulfuromonadales, Firmicutes, Bacteroidetes, Chlamydiae, Spirochaeta, and Chloroflexi and two archaeal groups, the Methanobacteriaceae and Archaeoglobaceae.

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte

scholarly journals Localization and Partial Characterization of an Aldehydic Component in Tropocollagen

1966 ◽  
Vol 241 (7) ◽  
pp. 1530-1536
Author(s):  
Marcos Rojkind ◽  
Olga O. Blumenfeld ◽  
Paul M. Gallop
Keyword(s):  
Partial Characterization

scholarly journals Detection and partial characterization of a protein in mouse fibroblasts that binds and inhibits trypsin.

1980 ◽  
Vol 255 (11) ◽  
pp. 5468-5474 ◽  
Author(s):  
R.J. Kirschner ◽  
C.S. Federiuk ◽  
J.P. Ford ◽  
J.A. Shafer
Keyword(s):  
Partial Characterization ◽  
Mouse Fibroblasts
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