scholarly journals Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160

2011 ◽  
Vol 2 ◽  
Author(s):  
Shinji Yoshizaki
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Specific Immune Response ◽  
Induced Pluripotent ◽  
Hiv 1

Generation of HIV-1-infected patients’ gene-edited induced pluripotent stem cells using feeder-free culture conditions

AIDS ◽  
2020 ◽  
Vol 34 (8) ◽  
pp. 1127-1139
Author(s):  
Lin Ye ◽  
Jiaming Wang ◽  
Fernando Teque ◽  
Fei Xie ◽  
Yuting Tan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Culture Conditions ◽  
Induced Pluripotent ◽  
Hiv 1

scholarly journals CCR5 Disruption in Induced Pluripotent Stem Cells Using CRISPR/Cas9 Provides Selective Resistance of Immune Cells to CCR5-tropic HIV-1 Virus

2015 ◽  
Vol 4 ◽  
pp. e268 ◽  
Author(s):  
HyunJun Kang ◽  
Petra Minder ◽  
Mi Ae Park ◽  
Walatta-Tseyon Mesquitta ◽  
Bruce E Torbett ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Immune Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Hiv 1

scholarly journals Lack of Immune Response to Differentiated Cells Derived from Syngeneic Induced Pluripotent Stem Cells

2017 ◽  
Vol 21 (1) ◽  
pp. 144-148 ◽  
Author(s):  
Prajna Guha ◽  
John W. Morgan ◽  
Gustavo Mostoslavsky ◽  
Neil P. Rodrigues ◽  
Ashleigh S. Boyd
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Differentiated Cells ◽  
Induced Pluripotent

scholarly journals Deprivation of human natural killer cells and antitumor immune response

2014 ◽  
Vol 2 ◽  
Author(s):  
Vyacheslav Ogay ◽  
Aliya Sekenova ◽  
Inpyo Choi
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Nk Cells ◽  
Induced Pluripotent Stem Cells ◽  
Natural Killer ◽  
Pluripotent Stem Cells ◽  
Antitumor Immune Response ◽  
Efficient Treatment ◽  
Cd34 Cells ◽  
Induced Pluripotent

Introduction: Cell-based immunotherapy has been given increased attention as a treatment for cancer. Human natural killer (NK) cells are resident lymphocyte populations. They exhibit potent antitumor activity without human leukocyte antigen matching and without prior antigen exposure. They also are a promising tool for immunotherapy of solid and hematologic cancers. However, most cancer patients do not have enough NK cells to induce an effective antitumor immune response. This demonstrates a need for a source of NK cells that can supplement the endogenous cell population.Material and methods: In this study, we derived induced pluripotent stem cells (iPSCs) from peripheral blood T-lymphocytes using Sendai virus vectors.Results: Generated iPSCs exhibited monoclonal T cell receptors (TCR) rearrangement in their genome, a hallmark of mature terminally differentiated T cells. These iPSCs were differentiated into NK cells using a two-stage coculture system: iPSCs into hematopoietic CD34+ cells with feeder cells M210-B4 (ATCC, USA) and CD34+ cells into mature NK cells with AFT024 cells (ATCC, USA). Our results showed that iPSC-derived NK cells expressed CD56, CD16, NKp 44 and NKp 46, possessed high cytotoxic activity  and produced high level of interferon-γ.Conclusion: Based on our data, derivation of NK cells from induced pluripotent stem cells should be considered in the treatment of oncologic diseases.This would allow for the development of cell therapy for cancer using immunologically compatible NK cells derived from iPSCs. This may contribute to a more efficient treatment of oncologic diseases in addition to traditional cancer treatment.

Generation of HIV Resistant and Functional Macrophages From Hematopoietic Stem Cell Derived Induced Pluripotent Stem Cells

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 562-562
Author(s):  
Amal Kambal ◽  
Gaela Mitchell ◽  
Whitney Cary ◽  
William Gruenloh ◽  
Yunjoon Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Conflicts Of Interest ◽  
Lentiviral Vector ◽  
Patient Specific ◽  
Hematopoietic Stem ◽  
Induced Pluripotent ◽  
Anti Hiv ◽  
Hiv 1

Abstract Abstract 562 Induced pluripotent stem cells (iPSCs) have radically advanced the field of regenerative medicine by making possible the production of patient-specific pluripotent stem cells from adult individuals. By developing iPSCs to treat HIV, there is the potential for generating a continuous supply of therapeutic cells for transplantation into HIV infected patients. In this study, we have utilized human hematopoietic stem cells (HSCs) to generate anti-HIV gene expressing iPSCs for HIV gene therapy. HSCs were de-differentiated into continuously growing iPSC lines with four reprogramming factors and a combination anti-HIV lentiviral vector containing a CCR5 shRNA and a human/rhesus chimeric TRIM5α gene. Upon directed differentiation of the anti-HIV iPSCs towards the hematopoietic lineage, a robust quantity (>35%) of colony forming CD133+ HSCs were obtained. These cells were further differentiated into functional end-stage macrophages which displayed a normal phenotypic profile. Upon viral challenge, the anti-HIV iPSC derived macrophages exhibited strong protection (>3 logs) from HIV-1 infection. Here we demonstrate the ability of iPSCs to develop into HIV-1 resistant immune cells and highlight the potential use of iPSCs for HIV gene and cellular therapies. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Global Proteomic and Methylome Analysis in Human Induced Pluripotent Stem Cells Reveals Overexpression of a Human TLR3 Affecting Proper Innate Immune Response Signaling

Stem Cells ◽  
2019 ◽  
Vol 37 (4) ◽  
pp. 476-488 ◽  
Author(s):  
Jordi Requena ◽  
Ana Belen Alvarez-Palomo ◽  
Montserrat Codina-Pascual ◽  
Raul Delgado-Morales ◽  
Sebastian Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Induced Pluripotent Stem Cells ◽  
Innate Immune Response ◽  
Pluripotent Stem Cells ◽  
Innate Immune ◽  
Methylome Analysis ◽  
Induced Pluripotent

scholarly journals Generation of HIV-1 Resistant and Functional Macrophages From Hematopoietic Stem Cell–derived Induced Pluripotent Stem Cells

2011 ◽  
Vol 19 (3) ◽  
pp. 584-593 ◽  
Author(s):  
Amal Kambal ◽  
Gaela Mitchell ◽  
Whitney Cary ◽  
William Gruenloh ◽  
Yunjoon Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Induced Pluripotent Stem Cells ◽  
Hematopoietic Stem Cell ◽  
Pluripotent Stem Cells ◽  
Hematopoietic Stem ◽  
Induced Pluripotent ◽  
Hiv 1

scholarly journals A High-Throughput Method as a Diagnostic Tool for HIV Detection in Patient-Specific Induced Pluripotent Stem Cells Generated by Different Reprogramming Methods

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniela Hübscher ◽  
Sabine Rebs ◽  
Luis Haupt ◽  
Thomas Borchert ◽  
Celina Isabell Guessoum ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Mononuclear Cells ◽  
Sendai Virus ◽  
Patient Specific ◽  
Pathogenic Viruses ◽  
Induced Pluripotent ◽  
Hiv 1

Induced pluripotent stem cells (iPSCs) provide a unique opportunity for generation of patient-specific cells for use in translational purposes. We aimed to compare iPSCs generated by different reprogramming methods regarding their reprogramming efficiency, pluripotency capacity, and the possibility to use high-throughput PCR-based methods for detection of human pathogenic viruses. iPSCs from skin fibroblasts (FB), peripheral blood mononuclear cells (PBMCs), or mesenchymal stem cells (MSCs) were generated by using three different reprogramming systems including chromosomal integrating and nonintegrating methods. Reprogramming efficiencies were in accordance with the literature, indicating that the parental cell type and the reprogramming method play a major role for the reprogramming efficiencies (FB: STEMCCA: 1.30±0.18, Sendai virus: 1.37±0.01, and episomal plasmids: 0.04±0.02; PBMCs: Sendai virus: 0.002±0.001, episomal plasmids: 0) but result in the same characteristics of pluripotency. We found the highest reprogramming efficiencies for MSC with 3.32±1.2 by using episomal plasmids. Since GMP standard working procedures and screening units need virus contamination-free cell lines, we studied HIV-1 contamination in the generated iPSCs. We used the high-throughput cobas® 6800/8800 system, which is normally used for detection of HIV-1 in plasma of patients, and found that footprint-free reprogramming methods as episomal plasmids and Sendai virus are useful for the described virus detection method. This fast, cost-effective, robust, and reliable assay demonstrates the feasibility to use high-throughput PCR-based methods for detection of human pathogenic viruses in ps-iPSC lines that were generated with nongenome integrating reprogramming methods.

scholarly journals Lack of Immune Response to Differentiated Cells Derived from Syngeneic Induced Pluripotent Stem Cells

2013 ◽  
Vol 12 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Prajna Guha ◽  
John W. Morgan ◽  
Gustavo Mostoslavsky ◽  
Neil P. Rodrigues ◽  
Ashleigh S. Boyd
Keyword(s):  
Stem Cells ◽  
Immune Response ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Differentiated Cells ◽  
Induced Pluripotent
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