scholarly journals Grass Carp Laboratory of Genetics and Physiology 2 Serves As a Negative Regulator in Retinoic Acid-Inducible Gene I- and Melanoma Differentiation-Associated Gene 5-Mediated Antiviral Signaling in Resting State and Early Stage of Grass Carp Reovirus Infection

2017 ◽  
Vol 8 ◽  
Author(s):  
Youliang Rao ◽  
Quanyuan Wan ◽  
Chunrong Yang ◽  
Jianguo Su
Keyword(s):  
Retinoic Acid ◽  
Resting State ◽  
Grass Carp ◽  
Early Stage ◽  
Negative Regulator ◽  
Antiviral Signaling ◽  
Grass Carp Reovirus ◽  
Reovirus Infection ◽  
Inducible Gene

Targeting Heat Shock Protein 70 as an antiviral strategy against grass carp reovirus infection

2018 ◽  
Vol 247 ◽  
pp. 1-9 ◽  
Author(s):  
Li-Peng Shan ◽  
Xiao-Hui Chen ◽  
Fei Ling ◽  
Bin Zhu ◽  
Gao-Xue Wang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Grass Carp ◽  
Heat Shock Protein 70 ◽  
Grass Carp Reovirus ◽  
Reovirus Infection

Oral delivery of Bacillus subtilis spores expressing grass carp reovirus VP4 protein produces protection against grass carp reovirus infection

2019 ◽  
Vol 84 ◽  
pp. 768-780 ◽  
Author(s):  
Hongye Jiang ◽  
Qing Bian ◽  
Weiwei Zeng ◽  
Pengli Ren ◽  
Hengchang Sun ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Grass Carp ◽  
Oral Delivery ◽  
Grass Carp Reovirus ◽  
Reovirus Infection

scholarly journals Autophagy Inhibits Grass Carp Reovirus (GCRV) Replication and Protects Ctenopharyngodon idella Kidney (CIK) Cells from Excessive Inflammatory Responses after GCRV Infection

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1296
Author(s):  
Pengfei Chu ◽  
Libo He ◽  
Rong Huang ◽  
Lanjie Liao ◽  
Yongming Li ◽  
...  
Keyword(s):  
Virus Replication ◽  
Grass Carp ◽  
Inflammatory Responses ◽  
Early Stage ◽  
Tracer Tests ◽  
Type I Interferons ◽  
Ctenopharyngodon Idella ◽  
Type I ◽  
Grass Carp Reovirus ◽  
Cik Cells

Autophagy is an essential and highly conserved process in mammals, which is critical to maintaining physiological homeostasis, including cell growth, development, repair, and survival. However, the understanding of autophagy in fish virus replication is limited. In this study, we found that grass carp reovirus (GCRV) infection stimulated autophagy in the spleen of grass carp (Ctenopharyngodon idella). Moreover, both Western blot (WB) analysis and fluorescent tracer tests showed that GCRV infection induced the enhancement of autophagy activation in Ctenopharyngodon idella kidney (CIK) cells. Autophagy inducer rapamycin and autophagy inhibitor 3-MA pretreatment can inhibit and promote the proliferation of GCRV, respectively. In addition, grass carp autophagy-related gene 5 (CiATG5)-induced autophagy, as well as rapamycin, showed effects on GCRV replication in CIK cells. Transcriptome analysis revealed that the total number of differentially expressed genes (DEGs) in CiATG5 overexpression groups was less than that of the control during GCRV infection. Enrichment analysis showed that CiATG5 overexpression induced the enhancement of autophagy, lysosome, phagosome, and apoptosis in the early stage of GCRV infection, which led to the clearance of viruses. In the late stage, steroid biosynthesis, DNA replication, terpenoid backbone biosynthesis, and carbon metabolism were upregulated, which contributed to cell survival. Moreover, signaling pathways involved in the immune response and cell death were downregulated in CiATG5 overexpression groups. Further study showed that CiATG5 repressed the expression of inflammatory response genes, including cytokines and type I interferons. Taken together, the results demonstrate that autophagy represses virus replication and attenuates acute inflammatory responses to protect cells.

scholarly journals Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection

2018 ◽  
Vol 19 (2) ◽  
pp. 488 ◽  
Author(s):  
Geng Chen ◽  
Libo He ◽  
Lifei Luo ◽  
Rong Huang ◽  
Lanjie Liao ◽  
...  
Keyword(s):  
Grass Carp ◽  
Grass Carp Reovirus ◽  
Reovirus Infection

Inhibition of Grass Carp Reovirus Infection by DNA Aptamers against S10 Protein

2017 ◽  
Vol 29 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Hongru Liang ◽  
Xiaozhe Fu ◽  
Lihui Liu ◽  
Qiang Lin ◽  
Huizhi Guo ◽  
...  
Keyword(s):  
Grass Carp ◽  
Dna Aptamers ◽  
Grass Carp Reovirus ◽  
Reovirus Infection
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