scholarly journals Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species

2020 ◽  
Vol 11 ◽  
Author(s):  
Wei Cui ◽  
Xiaoye Jin ◽  
Yuxin Guo ◽  
Chong Chen ◽  
Wenqing Zhang ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Forensic Identification ◽  
Development And Validation ◽  
Short Tandem

scholarly journals The use of touch DNA analysis in forensic identification focusing on Short Tandem Repeat-Combined DNA Index System loci THO1, CSF1PO and TPOX

2020 ◽  
Author(s):  
Ahmad Yudianto ◽  
Indah Nuraini M. ◽  
Abdul Hadi Furqoni ◽  
Simon Martin Manyanza Nzilibili ◽  
Pudji Harjanto
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Index System ◽  
Dna Analysis ◽  
Forensic Identification ◽  
Buccal Swab ◽  
Dna Index ◽  
Touch Dna ◽  
Composite Gel ◽  
Short Tandem

Forensic identification through DNA analysis is an accurate diagnostic tool. Deoxyribonucleic Acid (DNA) analysis is via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short Tandem Repeat (STR). At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects, thereby transferring trace evidence to the objects. In this study DNA was obtained using “touch DNA” from two buccal smears and two smear from watches and cellphones from volunteers who had signed the consent form. Samples were isolated using DNAzol. The quantity of DNA obtained will be measured using a UV spectrophotometer. For DNA amplification using 3 STR CODIS loci namely TH01, CSF1PO, and TPOX. The last step is visualization using acrylamide gel and silver staining. Mean levels of DNA (UVVisible Spectrophotometer) were 167.89±85.71 μg/mL for the buccal swab, 59.19±5.58 μg/mL for the watch swab, and 38.09±2.12 μg/mL for the mobile swab; the purity of the buccal swab DNA was 1.79±0.71, of the watch swab 1.69±0.76, and of the mobile swab 1.53±0.56. Visualization of PCR products on Polyacrylamide Agarose Composite Gel Electrophoresis stained with Silver and amplified using the standard primers THOI, TPOX and CSF1PO for STR Combined DNA Index System (CODIS) showed a 100% detection of amplicons. Both the buccal swab, watch swab and handphone swabs had trace amount of DNA that was sufficient to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX.

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