scholarly journals Characterization of Genetic and Allelic Diversity Amongst Cultivated and Wild Lentil Accessions for Germplasm Enhancement

2020 ◽  
Vol 11 ◽  
Author(s):  
Ruwani Dissanayake ◽  
Shivraj Braich ◽  
Noel O. I. Cogan ◽  
Kevin Smith ◽  
Sukhjiwan Kaur
Keyword(s):  
Allelic Diversity ◽  
Germplasm Enhancement ◽  
Wild Lentil

Morphological and molecular characterization of teosinte derived maize population

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Amarjeet Kumar ◽  
N. K. Singh ◽  
Sneha Adhikari ◽  
Anjali Joshi
Keyword(s):  
Molecular Characterization ◽  
Quantitative Traits ◽  
Morphological Characterization ◽  
Linkage Groups ◽  
Germplasm Enhancement ◽  
Maize Germplasm ◽  
Maize Population ◽  
Quantitative Characters ◽  
Morphological And Molecular Characterization

Germplasm enhancement seems to be an essential part of a breeding programme to improve resiliency, adaptability and productivity of the crops. To develop and diversify the maize germplasm, teosinte a wild relative of maize was integrated in crossing programme and BC1F4 lines were developed. Five BC1F4 lines along with teosinte and maize inbred DI-103 were characterised using quantitative characters and molecular markers. Morphological characterization was done with the help of visual parameters and quantitative traits and for molecular characterization fifty six SSR markers were used. SSR data were analysed with the help of software Mapmaker and twelve linkage groups were generated. Maximum allelic contributions from parent teosinte were found in the introgressed line AM-5 (53.4%) followed by AM-12 (48.9 %), whereas, least contribution of 34.1 % was found in AM-7. The maximum genetic distance among the introgressed lines was observed between AM-2 and AM-9 (0.75) followed by AM-2 and AM-7 (0.70), AM-7 and AM-9 (0.70). The maximum number of cob was found in AM-5 (5.00) followed by AM-2 (4.00). Grain yield per plant was found highest for AM-2 (100.00 g) followed by AM-12 (80.00 g), while, least value was observed for AM-7 (42.00 g). The results indicated differential parental contributions which leads to diversification in the progenies derived from diverse crosses in maize and further opined that such crosses seems to be essential for creating adapted germplasm to whom breeders are looking for.

scholarly journals Cross-amplification and Characterization of Polymorphic Microsatellite Markers From Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd.

2010 ◽  
Vol 59 (1-6) ◽  
pp. 285-288 ◽  
Author(s):  
Stephen F. Omondi ◽  
O. G. Dangasuk ◽  
D. W. Odee ◽  
S. Cavers ◽  
D. P. Khasa
Keyword(s):  
Microsatellite Markers ◽  
Allelic Diversity ◽  
Breeding Systems ◽  
Acacia Senegal ◽  
Cross Amplification ◽  
Expected Heterozygosity ◽  
Potential Source ◽  
Polymorphic Microsatellite ◽  
Acacia Mellifera

Abstract Seven polymorphic microsatellite markers isolated from Acacia brevispica and Acacia mellifera were successfully cross-amplified in Acacia senegal. The loci were surveyed for polymorphism using 30 samples. Allelic diversity ranged from 4 (Ame02, Ab06 and Ab18) to 13 (Ab26) per locus. The expected heterozygosity (HE) ranged from 0.543 (Ame02) to 0.868 (Ab26) while observed heterozygosity (HO) ranged from 0.516 (Ame05) to 0.800 (Ame03). Cross-amplification of these loci represents a potential source of co-dominant markers and will be useful in the study of genetic diversity, structure, gene flow and breeding systems of this important Acacia species.

scholarly journals Mutant Selection Window and Characterization of Allelic Diversity for Ciprofloxacin-Resistant Mutants of Rhodococcus equi

2010 ◽  
Vol 54 (8) ◽  
pp. 3520-3523 ◽  
Author(s):  
Hidekazu Niwa ◽  
Brent A. Lasker
Keyword(s):  
Amino Acid ◽  
Allelic Diversity ◽  
Dna Gyrase ◽  
Rhodococcus Equi ◽  
Mutant Selection ◽  
Single Nucleotide ◽  
Selection Window ◽  
Mutant Prevention Concentration ◽  
Resistant Mutants

ABSTRACT The mutant prevention concentration (MPC) for ciprofloxacin was determined for two Rhodococcus equi strains. The MPC for both strains was 32 μg/ml, which is above the peak serum concentration of ciprofloxacin obtainable by oral administration in humans. Nine single nucleotide changes corresponding to eight amino acid substitutions in the quinolone resistance-determining regions of DNA gyrase subunits A and B were characterized. Only mutants with amino acid changes in Ser-83 of GyrA were highly resistant (≥64 μg/ml). Our results suggest that ciprofloxacin monotherapy against R. equi infection may result in the emergence of ciprofloxacin-resistant mutants.

Characterization of Microsatellites in Bambusa arundinacea and Cross Species Amplification in Other Bamboos

2005 ◽  
Vol 60 (7-8) ◽  
pp. 605-610 ◽  
Author(s):  
Sumitra Nayak ◽  
Gyana Ranjan Rout
Keyword(s):  
Tandem Repeats ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Bamboo Species ◽  
The Family ◽  
Ssr Loci ◽  
Polymerase Chain ◽  
Genetic Diversity Study ◽  
First Time

Microsatellites, tandem repeats of short nucleotide (1-6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within genome, ease of genotyping through polymerase chain reaction (PCR), selectively neutral, co-dominant and multi allelic nature. Six microsatellites, three polymorphic and three monomorphic, have been characterized for the first time in a bamboo species, Bambusa arudinacea belonging to the family Poaceae. The number of alleles per locus ranges form 2 to 13. Allelic diversity ranges from 0.041 to 0.870. Polymorphic information content (PIC) values for two loci were > 0.3, an indicator of polymorphic allele. Cross species amplification has been tested in other 18 bamboo species. Monomorphic simple sequence repeats (SSRs) have been found to be cross amplified in most of the tested species while polymorphic ones in only three to four species. The utility of the SSR loci in genetic diversity study of B. arundinacea and other cross amplified bamboo species have been discussed.

scholarly journals Development and use of miRNA-derived SSR markers for the study of genetic diversity, population structure, and characterization of genotypes for breeding heat tolerant wheat varieties

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0231063
Author(s):  
Sandhya Tyagi ◽  
Anuj Kumar ◽  
Tinku Gautam ◽  
Renu Pandey ◽  
Sachin Rustgi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Heat Stress ◽  
Ssr Markers ◽  
Allelic Diversity ◽  
Abiotic Factor ◽  
Wheat Genotypes ◽  
Mirna Genes ◽  
Heat Tolerant

Heat stress is an important abiotic factor that limits wheat production globally, including south-east Asia. The importance of micro (mi) RNAs in gene expression under various biotic and abiotic stresses is well documented. Molecular markers, specifically simple sequence repeats (SSRs), play an important role in the wheat improvement breeding programs. Given the role of miRNAs in heat stress-induced transcriptional regulation and acclimatization, the development of miRNA-derived SSRs would prove useful in studying the allelic diversity at the heat-responsive miRNA-genes in wheat. In the present study, efforts have been made to identify SSRs from 96 wheat heat-responsive miRNA-genes and their characterization using a panel of wheat genotypes with contrasting reactions (tolerance/susceptible) to heat stress. A set of 13 miRNA-derived SSR markers were successfully developed as an outcome. These miRNA-SSRs are located on 11 different common wheat chromosomes (2A, 3A, 3B, 3D, 4D, 5A, 5B, 5D, 6A, 6D, and 7A). Among 13 miRNA-SSRs, seven were polymorphic on a set of 37 selected wheat genotypes. Within these polymorphic SSRs, three makers, namely HT-169j, HT-160a, and HT-160b, were found promising as they could discriminate heat-tolerant and heat-susceptible genotypes. This is the first report of miRNA-SSR development in wheat and their deployment in genetic diversity and population structure studies and characterization of trait-specific germplasm. The study suggests that this new class of molecular makers has great potential in the marker-assisted breeding (MAB) programs targeted at improving heat tolerance and other adaptability or developmental traits in wheat and other crops.

OR33 Allelic diversity and haplotype structure of HLA-DPB1 based on full-length characterization of most CWD alleles

2016 ◽  
Vol 77 ◽  
pp. 31
Author(s):  
Gerhard Schöfl ◽  
Kathrin Lang ◽  
Marie Günther ◽  
Grit Schober ◽  
Carolin Massalski ◽  
...  
Keyword(s):  
Allelic Diversity ◽  
Full Length ◽  
Haplotype Structure
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