scholarly journals Ex-situ Bioremediation of U(VI) from Contaminated Mine Water Using Acidithiobacillus ferrooxidans Strains

2016 ◽  
Vol 4 ◽  
Author(s):  
Maria Romero-González ◽  
Bennett C. Nwaobi ◽  
Joseph M. Hufton ◽  
Daniel J. Gilmour
Keyword(s):  
Mine Water ◽  
Acidithiobacillus Ferrooxidans ◽  
Ex Situ ◽  
Acidithiobacillus Ferrooxidans Strains

Enrichment of "Ferrovum" spp. and Gallionella Relatives Using Artificial Mine Water

2013 ◽  
Vol 825 ◽  
pp. 54-57 ◽  
Author(s):  
Judith Kipry ◽  
Rawaa Jaffer Jwair ◽  
Nadja Gelhaar ◽  
Claudia Wiacek ◽  
Eberhard Janneck ◽  
...  
Keyword(s):  
Microbial Community ◽  
Mine Water ◽  
Acidithiobacillus Ferrooxidans ◽  
Pilot Plant ◽  
Iron Oxidation ◽  
Iron Phosphate ◽  
Lower Number ◽  
Mine Waters ◽  
Culture Independent ◽  
Respective Species

New acidophilic iron-oxidising bacteria likeFerrovumspp. andGallionellarelatives have recently been detected in several mine waters by culture-independent methods. To get information about their physiological characteristics, isolation of respective species is required. In former isolation studies, using a previously described iron-containing medium (iFeo) combined with the overlay-plate technique, mainlyAcidithiobacillus ferrooxidansorA. ferrivoranshad been obtained, althoughF. myxofaciensdominated the microbial community. Therefore, a new medium, called APPW was designed based on the water chemistry of a pilot plant for iron oxidation. The growth of iron-oxidising bacteria on overlay plates with iFeo and APPW media, respectively, was compared. Additionally, the iron, phosphate, and ammonium concentrations of APPW were separately adapted to iFeo. Colonies grown were analysed by PCR with primers specific forFerrovumspp.,Gallionellarelatives,Acidithiobacillusspp., andAcidiphiliumspp. While on all media most colonies obtained were contaminated by heterotrophicAcidiphiliumspp., on iFeoAcidithiobacillus, as expected, turned out to be the dominating genus among the iron oxidisers. Interestingly on APPW and APPW-NH4Ferrovumdominated among the iron oxidisers. While APPW-Fe, as compared to APPW, seemed to inhibit theGallionellarelatives, APPW-PO4resulted in an overall lower number of colonies, but a higher percentage of this taxon.

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