scholarly journals Screening for the Genes Involved in Bombykol Biosynthesis: Identification and Functional Characterization of Bombyx mori Acyl Carrier Protein

2011 ◽  
Vol 2 ◽  
Author(s):  
Atsushi Ohnishi ◽  
Misato Kaji ◽  
Kana Hashimoto ◽  
Shogo Matsumoto
Keyword(s):  
Bombyx Mori ◽  
Acyl Carrier Protein ◽  
Functional Characterization ◽  
Carrier Protein

scholarly journals Transcriptional and functional characterization of the gene encoding acyl carrier protein in Bacillus subtilis

Microbiology ◽  
2010 ◽  
Vol 156 (2) ◽  
pp. 484-495 ◽  
Author(s):  
Mariano A. Martinez ◽  
Diego de Mendoza ◽  
Gustavo E. Schujman
Keyword(s):  
Bacillus Subtilis ◽  
Fatty Acid ◽  
Molecular Mechanisms ◽  
Fatty Acid Biosynthesis ◽  
Acyl Carrier Protein ◽  
Functional Characterization ◽  
Carrier Protein ◽  
Normal Fatty Acid ◽  
Gram Positive

Acyl carrier protein (ACP) is a universal and highly conserved carrier of acyl intermediates during fatty acid biosynthesis. The molecular mechanisms of regulation of the acpP structural gene, as well as the function of its gene product, are poorly characterized in Bacillus subtilis and other Gram-positive organisms. Here, we report that transcription of acpP takes place from two different promoters: PfapR and PacpP. Expression of acpP from PfapR is coordinated with a cluster of genes involved in lipid synthesis (the fapR operon); the operon consists of fapR-plsX-fabD-fabG-acpP. PacpP is located immediately upstream of the acpP coding sequence, and is necessary and sufficient for normal fatty acid synthesis. We also report that acpP is essential for growth and differentiation, and that ACP localizes in the mother-cell compartment of the sporangium during spore formation. These results provide the first detailed characterization of the expression of the ACP-encoding gene in a Gram-positive bacterium, and highlight the importance of this protein in B. subtilis physiology.

scholarly journals Cloning and functional characterization of an acyl-acyl carrier protein thioesterase (JcFATB1) from Jatropha curcas

2009 ◽  
Vol 29 (10) ◽  
pp. 1299-1305 ◽  
Author(s):  
P.-Z. Wu ◽  
J. Li ◽  
Q. Wei ◽  
L. Zeng ◽  
Y.-P. Chen ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Acyl Carrier Protein ◽  
Functional Characterization ◽  
Carrier Protein

Molecular cloning and characterization of two β-ketoacyl-acyl carrier protein synthase I genes from Jatropha curcas L.

2017 ◽  
Vol 214 ◽  
pp. 152-160 ◽  
Author(s):  
Wangdan Xiong ◽  
Qian Wei ◽  
Pingzhi Wu ◽  
Sheng Zhang ◽  
Jun Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Jatropha Curcas ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Jatropha Curcas L
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