scholarly journals MicroRNA-29b/c-3p Indicate Advanced Liver Fibrosis/Cirrhosis in Univentricular Heart Patients With and Without Fontan Palliation

2021 ◽  
Vol 7 ◽  
Author(s):  
Masood Abu-Halima ◽  
Eckart Meese ◽  
Mohamad Ali Saleh ◽  
Andreas Keller ◽  
Hashim Abdul-Khaliq ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Statistical Significance ◽  
Univentricular Heart ◽  
Heart Patients ◽  
Hepatic Congestion ◽  
Collapsibility Index ◽  
The Status ◽  
Advanced Liver Fibrosis ◽  
End Stage ◽  
Fontan Palliation

Aim: The present study aims to identify those microRNAs (miRNAs) in patients with univentricular heart (UVH) disease with and without Fontan palliation that may be associated with advanced liver fibrosis/cirrhosis.Materials and Methods: SurePrint™ 8 × 60K Human v21 miRNA arrays were used to determine the miRNA abundance profiles in the blood of 48 UVH patients with and without Fontan palliation and 32 matched healthy controls. The abundance levels of selected miRNAs have been validated by quantitative reverse transcription-polymerase chain reaction (RT-qPCR).Results: According to microarray analysis, 50 miRNAs were found to be significantly abundant in UVH patients of which miR-29b-3p and miR-29c-3p were significantly related to the model of end-stage liver disease (MELD)-Albumin and albumin-bilirubin (ALBI) score representing advanced liver fibrosis/cirrhosis. Relative expression levels of both miRNAs were significantly higher in patients with a higher collapsibility index representing venous hepatic congestion, a higher MELD-Albumin or ALBI score and incomplete or no Fontan palliation. In the logistic regression analysis, a MELD-Albumin score ≥ 11 or ALBI score > −2.6 were best predicted by total bilirubin (OR 6.630, P = 0.016), albumin (OR 0.424, P = 0.026), and miR-29c-3p (OR 33.060, P = 0.047). After adjustment to the status of Fontan palliation, however, no statistical significance of these parameters was found thus underlining the importance of palliation status on progression of liver fibrosis/ cirrhosis in UVH patients.Conclusions: In UVH patients with and without Fontan palliation, miR-29b-3p and miR-29c-3p seem to be markers of advanced liver fibrosis/cirrhosis and thus may be used in the risk assessment of these patients.

scholarly journals MicroRNA 29c-3p indicates advanced liver fibrosis/cirrhosis in univentricular heart patients with and without Fontan palliation

2020 ◽  
Author(s):  
Masood Abu-Halima ◽  
Eckart Meese ◽  
Mohamad Ali Saleh ◽  
Andreas Keller ◽  
Hashim Abdul-Khaliq ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Fibrosis ◽  
Microarray Analysis ◽  
Independent Predictor ◽  
Prognostic Scores ◽  
Univentricular Heart ◽  
Collapsibility Index ◽  
Advanced Liver Fibrosis ◽  
And Gender ◽  
Fontan Palliation

Abstract Background: In patients with chronic liver disease that usually results in liver fibrosis, microRNAs (miRNAs) have been shown to be of diagnostic and prognostic value. The present study aims to identify those miRNAs in patients with univentricular heart (UVH) disease with and without Fontan palliation that may be associated with advanced liver fibrosis/cirrhosis.Methods: A large panel of human miRNA arrays were used to determine miRNA abundance profiles in the blood of 48 UVH patients with and without Fontan palliation and 32 age and gender-matched healthy controls. The most abundantly expressed miRNAs identified by microarray analysis were correlated to prognostic scores of advanced liver fibrosis/cirrhosis and selected miRNAs validated by RT-qPCR.Results: According to microarray analysis, 50 miRNAs were found to be significantly abundant in UVH patients of which miR-29b-3p and miR-29c-3p were significantly correlated to the model of end-stage liver disease (MELD)-Albumin and albumin-bilirubin (ALBI) score. According to ROC analysis, a MELD-Albumin score ≥ 11 was best predicted by total bilirubin (AUC 0.985, P=0.005), collapsibility index (AUC 0.952, P=0.009) and miR-29c-3p (AUC 0.941, P=0.011) and the most significant predictors of an ALBI score >−2.6 were collapsibility index (AUC 0.998, P<0.001) and albumin (AUC 0.981, P=0.006). In the multivariate analysis, miR-29c-3p turned out to be an independent predictor of advanced liver fibrosis/cirrhosis as defined by a MELD-Albumin score ≥ 11 (P=0.004) and ALBI score > −2.6 (P=0.009), respectively.Conclusion: In UVH patients with and without Fontan palliation, miR-29c-3p seems to be an independent predictor of advanced liver fibrosis/cirrhosis and thus may be used in the risk assessment of these patients.

scholarly journals Diagnostic accuracy of aspartate aminotransferase to platelet ratio index and fibrosis 4 scores in predicting advanced liver fibrosis in patients with end-stage renal disease and chronic viral hepatitis: Experience from Pakistan

2018 ◽  
Vol 6 (1) ◽  
pp. 38-42 ◽  
Author(s):  
Rajesh Kumar Wadhva ◽  
Muhammad Manzoorul Haque ◽  
Nasir Hassan Luck ◽  
Abbas Ali Tasneem ◽  
Zaigham Abbas ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Biopsy ◽  
Chronic Viral Hepatitis ◽  
Advanced Fibrosis ◽  
Advanced Liver Fibrosis ◽  
Apri Score ◽  
Stage Renal Disease ◽  
End Stage ◽  
Sensitivity Specificity ◽  
Esrd Patients

Abstract Objectives The aim was to assess the diagnostic accuracy of APRI and FIB-4 in assessing the stage of liver fibrosis in end stage renal disease (ESRD) patients with chronic viral hepatitis and to compare the two tests with standard tru-cut liver biopsy. Material and Methods The study was conducted at Sindh Institute of Urology and Transplantation Karachi (SIUT) from May 2010 to May 2014. All ESRD patients, being considered as candidates for renal transplantation and in whom liver biopsy was performed were included. Fibrosis stage was assessed on liver biopsy using Ishak scoring system. The serum transaminases and platelet counts were used to calculate APRI and FIB-4 scores. Results Out of 109 patients, hepatitis C and B virus infections were present in 104 (95.4%) and 3(2.8%), respectively, while 2 (1.8%) patients had both infections. The mean Ishak fibrosis score was 1.95 ± 2. Advanced fibrosis was noted in 37 (34%) patients. Univariate analysis showed that advanced liver fibrosis was associated with lower platelets counts (P=0.001) and higher aspartate aminotransferase (AST) (P=0.001), alanine aminotransferase (ALT) (P=0.022), APRI score (P=0.001) and FIB-4 score (P=0.001). On logistic regression analysis, only APRI score (P < 0.001) was found to be the independent variable associated with advanced liver fibrosis. APRI score cutoff ≥1 indicating advanced fibrosis showed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 91.9%, 90.3%, 82.9%, 95.6%, respectively with area under the curve (AUC) of 0.97. Similarly, a FIB-4 score cutoff ≥1.1 had sensitivity, specificity, PPV and NPV of 70.27%, 66.67%, 52% and 81.36%, respectively with AUC of 0.74. Conclusion APRI is more accurate noninvasive test for assessing advanced liver fibrosis in ESRD patients as compared to FIB-4. It can be used to obviate the need for liver biopsy in this high risk population.

scholarly journals Prediction of Nonalcoholic Fatty Liver Disease Using Noninvasive and Non-Imaging Procedures in Japanese Health Checkup Examinees

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 132
Author(s):  
Kenichiro Murayama ◽  
Michiaki Okada ◽  
Kenichi Tanaka ◽  
Chika Inadomi ◽  
Wataru Yoshioka ◽  
...  
Keyword(s):  
Liver Disease ◽  
Liver Fibrosis ◽  
Fatty Liver ◽  
Japanese Population ◽  
Fatty Liver Disease ◽  
Health Checkup ◽  
Nonalcoholic Fatty Liver ◽  
General Japanese Population ◽  
Advanced Liver Fibrosis ◽  
Zju Index

Access to imaging is limited for diagnosing nonalcoholic fatty liver disease (NAFLD) in general populations. This study evaluated the diagnostic performance of noninvasive and nonimaging indexes to predict NAFLD in the general Japanese population. Health checkup examinees without hepatitis virus infection or habitual alcohol drinking were included. Fatty liver was diagnosed by ultrasonography. The hepatic steatosis index (HSI), Zhejiang University (ZJU) index, and fatty liver index (FLI) were determined, and risk of advanced liver fibrosis was evaluated by the fibrosis-4 index. NAFLD was diagnosed in 1935 (28.0%) of the 6927 subjects. The area under the receiver operating characteristic (AUROC) curve of the HSI, ZJU index, and FLI was 0.874, 0.886, and 0.884, respectively. The AUROC of the ZJU index (p < 0.001) and FLI (p = 0.002) was significantly greater than that for the HSI. In subjects with a high risk of advanced fibrosis, the sensitivity of the HSI, ZJU index, and FLI were 88.8%, 94.4%, and 83.3% with a low cut-off value and the specificity was 98.5%, 100%, and 100% with a high cut-off value. In conclusion, all indexes were useful to diagnose NAFLD in the general Japanese population and in subjects with potentially advanced liver fibrosis.

scholarly journals A blood-based prognostic liver secretome signature and long-term hepatocellular carcinoma risk in advanced liver fibrosis

Med ◽  
2021 ◽  
Author(s):  
Naoto Fujiwara ◽  
Masahiro Kobayashi ◽  
Austin J. Fobar ◽  
Ayaka Hoshida ◽  
Cesia A. Marquez ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Fibrosis ◽  
Advanced Liver Fibrosis ◽  
Carcinoma Risk

scholarly journals Calcium Metabolism following Renal Transplantation

1994 ◽  
Vol 31 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Anne M Straffen ◽  
DJS Carmichael ◽  
Angela Fairney ◽  
B Hulme ◽  
M Snell
Keyword(s):  
Renal Transplantation ◽  
Calcium Homeostasis ◽  
End Stage Renal Disease ◽  
Post Transplantation ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D ◽  
Transplant Patients ◽  
The Status ◽  
Stage Renal Disease ◽  
End Stage

Abnormalities of calcium homeostasis are a recognized feature of end-stage renal disease. The treatment of choice is renal transplantation, but this does not always result in normalization of the biochemical profile. Persistent hypercalcaemia is well documented and our study was undertaken to investigate the status of the calcium regulating hormones in renal patients post-transplantation. Serum calcium, parathyroid hormone, 1,25-dihydroxyvitamin D (1,25(OH)2D) and osteocalcin concentrations were measured in post-transplant patients. Twenty per cent of the patients had subnormal 1,25(OH)2D concentrations while 55% had biochemical evidence of hyperparathyroidism but only 5% were hypercalcaemic. Time elapsed since transplantation was not correlated with any of the analytes investigated and there was no relationship between persistent impairment of renal function and abnormalities of calcium homeostasis.

Sa1547 Value of 2D-SWE.GE for the Assessement of Liver Stiffness in Advanced Liver Fibrosis

2016 ◽  
Vol 150 (4) ◽  
pp. S1064
Author(s):  
Felix Bende ◽  
Alina Popescu ◽  
Roxana Sirli ◽  
Raluca Lupusoru ◽  
Ruxandra G. Mare ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Liver Stiffness ◽  
Advanced Liver Fibrosis

scholarly journals Thiazolidinediones and Advanced Liver Fibrosis in Nonalcoholic Steatohepatitis

2017 ◽  
Vol 177 (5) ◽  
pp. 633 ◽  
Author(s):  
Giovanni Musso ◽  
Maurizio Cassader ◽  
Elena Paschetta ◽  
Roberto Gambino
Keyword(s):  
Liver Fibrosis ◽  
Nonalcoholic Steatohepatitis ◽  
Advanced Liver Fibrosis
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