scholarly journals Regulation of Host Innate Immunity by Non-Coding RNAs During Dengue Virus Infection

2020 ◽  
Vol 10 ◽  
Author(s):  
Roopali Rajput ◽  
Jitender Sharma ◽  
Mahima T. Nair ◽  
Madhu Khanna ◽  
Pooja Arora ◽  
...  
Keyword(s):  
At Risk ◽  
Virus Infection ◽  
Dengue Virus ◽  
Viral Infections ◽  
Clinical Manifestations ◽  
Dengue Virus Infection ◽  
Replication Process ◽  
Diagnostic Strategies ◽  
Non Coding Rnas ◽  
Essential Knowledge

An estimated 3.9 billion individuals in 128 nations (about 40% of global population) are at risk of acquiring dengue virus infection. About 390 million cases of dengue are reported each year with higher prevalence in the developing world. A recent modeling-based report suggested that half of the population across the globe is at risk of dengue virus infection. In any given dengue outbreak, a percentage of infected population develops severe clinical manifestations, and this remains one of the “unsolved conundrums in dengue pathogenesis”. Although, host immunity and virus serotypes are known to modulate the infection, there are still certain underlying factors that play important roles in modulating dengue pathogenesis. Advanced genomics-based technologies have led to identification of regulatory roles of non-coding RNAs. Accumulating evidence strongly suggests that viruses and their hosts employ non-coding RNAs to modulate the outcome of infection in their own favor. The foremost ones seem to be the cellular microRNAs (miRNAs). Being the post-transcriptional regulators, miRNAs can be regarded as direct switches capable of turning “on” or “off” the viral replication process. Recently, role of long non-coding RNAs (lncRNAs) in modulating viral infections via interferon dependent or independent signaling has been recognized. Hence, we attempt to identify the “under-dog”, the non-coding RNA regulators of dengue virus infection. Such essential knowledge will enhance the understanding of dengue virus infection in holistic manner, by exposing the specific molecular targets for development of novel prophylactic, therapeutic or diagnostic strategies.

scholarly journals GAMBARAN NILAI HEMATOKRIT DAN LAJU ENDAP DARAH PADA ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Sitti S. P. Kamuh ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Symptoms ◽  
Clinical Manifestations ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Dengue Virus Infection ◽  
Erythrocyte Sedimentation

Abstract: Dengue is a major public health problem that can be found throughout the tropical and subtropical countries around the world. Spectrum of clinical manifestations of dengue virus infection varies greatly. In addition to clinical symptoms, the diagnosis of dengue virus infection need to be supported by blood tests such as hematocrit and erythrocyte sedimentation rate. This study aimed to determine the hematocrit value and erythrocyte sedimentation rate (ESR) in children with dengue virus infection in Manado. This study used a cross-sectional design and was conducted from Desember 2014 to January 2015 at GMIM Pancaran Kasih, Advent, and Robert Wolter Mongisidi hospitals in Manado. There were 37 patients that fulfilled the inclusion criteria, consisted of 17 males and 20 females. The results showed that of 37 patients, there were 36 with hematocrit within normal limits. Moreover, of 37 patients only 6 patients performed the ESR examination; only 1 child had a rapid ESR result (> 15mm/h). Conclusion: In this study, most of the pediatric patients with dengue virus infection in Manado had normal hematocrit. Of 6 patients who had performed ESR test, only 1 had rapid ESR.Keywords: dengue, hematocrit, erythrocyte sedimentation rateAbstrak: Dengue merupakan masalah kesehatan masyarakat yang utama dan dapat ditemui diseluruh daerah tropis dan subtropis di seluruh dunia. Spektrum manifestasi klinis infeksi virus dengue sangat bervariasi. Selain gejala klinis, diagnosis infeksi virus dengue perlu ditunjang hasil uji darah di laboratorium antara lain hematokrit dan laju endap darah (LED). Penelitian ini bertujuan untuk mengetahui nilai hematokrit dan LED pada anak dengan infeksi virus dengue di Manado. Penelitian ini menggunakan desain potong lintang, dilakukan pada bulan Desember 2014 sampai Januari 2015 di RSU GMIM Pancaran Kasih Manado, RS Advent Manado, dan RSAD Robert Wolter Mongisidi Teling17 anak laki-laki dan 20 anak perempuan. Hasil penelitian memperlihatkan 36 dari 37 pasien mempunyai hematokrit normal. Dasri 37 pasien, hanya 6 anak yang menjalani pemeriksaan LED; hanya 1 anak dengan LED cepat (> 15mm/jam). Simpulan: Pada studi ini, sebagian besar pasien anak dengan infeksi virus dengue di Manado mempunyai nilai hematokrit normal. Dari 6 pasien yang dilakukan pemeriksaan LED, 1 anak mempunyai hasil LED cepat.Kata kunci: dengue, hematrokrit, laju endap darah.

scholarly journals Correlation of Serotype-Specific Dengue Virus Infection with Clinical Manifestations

2012 ◽  
Vol 6 (5) ◽  
pp. e1638 ◽  
Author(s):  
Eric S. Halsey ◽  
Morgan A. Marks ◽  
Eduardo Gotuzzo ◽  
Victor Fiestas ◽  
Luis Suarez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Dengue Virus Infection

TROMBOSIT TERENDAH, KENAIKAN HEMATOKRIT DAN KADAR TNF-α SEBAGAI INDIKASI KEPARAHAN INFEKSI VIRUS DENGUE PADA PASIEN DD DAN DBD

2019 ◽  
Vol 10 (01) ◽  
pp. 13-22
Author(s):  
A.S. Siskayani ◽  
I Made Sumarya ◽  
N.L.P Kartika Sari
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Hemorrhagic Fever ◽  
Hemorrhagic Diathesis ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Blood Samples ◽  
Tnf Α ◽  
The Mean

Clinical manifestations of dengue virus infection vary from unspecified fever, dengue fever (DF) and dengue hemorrhagic fever (DHF). The fundamental difference in the diagnosis between DF and DHF is plasma leakage, hypotension, thrombocytopenia and hemorrhagic diathesis in DHF. The objectives of the study were to determine the lowest platelet, haematocrit increase, and TNF-α levels in DF and DHF patients as an indication of severity of dengue virus infection. The cross-sectional study was conducted by taking 54 blood samples of patients aged 26-45 years who were infected by dengue virus and grouped into two groups: DF patient group (27 blood samples) and DHF group (27 blood samples). The lowest platelets and hematocrit rise were determined by Hematology Analyzer and the TNF-α levels were determined by ELISA. The data obtained were statistically analyzed by independent Mann-Whitney test. The results showed that the lowest mean platelet counts of DF patients were significantly (p <0.01) higher than those of DHF patients. The mean hematocrit increase in DF patients was significantly (p <0.01) lower than that of the DHF patients and the mean TNF-α levels of DF patients were significantly (p <0.01) lower than the DHF patients. Based on the results of this study it can be concluded that the lowest platelets, hematocrit increase and TNF-α levels are an indication of the severity of dengue virus infection.

scholarly journals Manifestation of thrombocytopenia in dengue-2-virus-infected mice

2000 ◽  
Vol 81 (9) ◽  
pp. 2177-2182 ◽  
Author(s):  
Kao-Jean Huang ◽  
Shu-Yi J. Li ◽  
Shiour-Ching Chen ◽  
Hsiao-Sheng Liu ◽  
Yee-Shin Lin ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Dengue Virus Infection ◽  
Platelet Antibody

Dengue virus infection causes dengue fever, dengue haemorrhagic fever and dengue shock syndrome. No animal model is available that mimics these clinical manifestations. In this study, the establishment is reported of a murine model for dengue virus infection that resembles the thrombocytopenia manifestation. Dengue-2 virus (dengue virus type 2) can infect murine cells either in vitro (primary cell culture) or in vivo. Viraemia detected by RT–PCR was found transiently at 2 days after intravenous injection of dengue-2 virus. Transient thrombocytopenia developed at 10–13 days after primary or secondary infection. Anti-platelet antibody was generated after dengue-2 virus infection. There was strain variation in dengue-2 virus infection; the A/J strain was more sensitive than BALB/c or B6 mice. This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of dengue virus infection.

scholarly journals Sera-epidemiology of Dengue Virus Infection in Clinically Suspected Patients Attended in Dhaka Medical College Hospital During January to December 2016

2018 ◽  
Vol 26 (2) ◽  
pp. 111-116
Author(s):  
Monira Pervin ◽  
Afroza Akbar Sweety ◽  
Mohammad Zaid Hossain ◽  
Rabeya Sharmin ◽  
Nusrat Fatema ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Clinical Manifestations ◽  
Medical College Hospital ◽  
Dengue Virus Infection ◽  
College Hospital ◽  
Medical College ◽  
Igm Antibody ◽  
Orbital Pain

Introduction: Dengue is now established as one of the most important Arboviral infection in tropical countries. As the epidemic continues worldwide, this Aedes mosquito-transmitted pathogen is considered a major re-emerging tropical disease and significant public health concern. The study was conducted to determine the sero-epidemiology and clinical picture of dengue virus infection among the clinically suspected patients attended in Dhaka Medical College Hospital during January to December 2016.Materials and Methods: The study was carried out on 145 clinically suspected Dengue patients attended in Dhaka Medical College Hospital from January to December 2016 who had fever with temperature >39°C. Blood sample was collected during acute febrile phase of patients and separated sera were tested for NS1 dengue antigen and IgM antibodies using commercial test kits (NS1 by OMC Healthcare (Pvt.) Ltd & IgM antibody by Omega Diagnostics Ltd.).Results: Of the total 145 patients 40 (27.6%) were positive for Dengue NS1 antigen and or IgM antibody test. Of the positive 40 patients, 18 (45%) were positive for NS1, 19 (47.5%) were positive for IgM and 3 (7.5%) were positive for both NS1 and IgM antibody. Major clinical manifestations were fever with arthralgia (79.5%) and retro-orbital pain (50%).Conclusion: Outbreak of Dengue fever is continuing every year and typical presentation of Dengue fever has changed. Arthralgia and retro orbital pain was the most common clinical feature in the present study instead of headache and rash with fever. A large number of suspected dengue cases were negative by Dengue tests for antigen NS1 and IgM antibody which suggests other organisms of similar clinical manifestations are becoming prevalent in Dhaka city.J Dhaka Medical College, Vol. 26, No.2, October, 2017, Page 111-116

scholarly journals RIG-I Activation by a Designer Short RNA Ligand Protects Human Immune Cells against Dengue Virus Infection without Causing Cytotoxicity

2019 ◽  
Vol 93 (14) ◽  
Author(s):  
Victor Ho ◽  
Hui Yee Yong ◽  
Marion Chevrier ◽  
Vipin Narang ◽  
Josephine Lum ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Human Skin ◽  
Immune Cells ◽  
Viral Infections ◽  
Short Hairpin Rna ◽  
Dengue Virus Infection ◽  
Hairpin Rna ◽  
Short Hairpin ◽  
Rna Ligands

ABSTRACT Virus-derived double-stranded RNA (dsRNA) molecules containing a triphosphate group at the 5′ end are natural ligands of retinoic acid-inducible gene I (RIG-I). The cellular pathways and proteins induced by RIG-I are an essential part of the innate immune response against viral infections. Starting from a previously published RNA scaffold (3p10L), we characterized an optimized small dsRNA hairpin (called 3p10LG9, 25 nucleotides [nt] in length) as a highly efficient RIG-I activator. Dengue virus (DENV) infection in cell lines and primary human skin cells could be prevented and restricted through 3p10LG9-mediated activation of RIG‐I. This antiviral effect was RIG-I and interferon signal dependent. The effect was temporary and was reversed above a saturating concentration of RIG-I ligand. This finding revealed an effective feedback loop that controls potentially damaging inflammatory effects of the RIG-I response, at least in immune cells. Our results show that the small RIG-I activator 3p10LG9 can confer short-term protection against DENV and can be further explored as an antiviral treatment in humans. IMPORTANCE Short hairpin RNA ligands that activate RIG-I induce antiviral responses in infected cells and prevent or control viral infections. Here, we characterized a new short hairpin RNA molecule with high efficacy in antiviral gene activation and showed that this molecule is able to control dengue virus infection. We demonstrate how structural modifications of minimal RNA ligands can lead to increased potency and a wider window of RIG-I-activating concentrations before regulatory mechanisms kick in at high concentrations. We also show that minimal RNA ligands induce an effective antiviral response in human skin dendritic cells and macrophages, which are the target cells of initial infection after the mosquito releases virus into the skin. Using short hairpin RNA as RIG-I ligands could therefore be explored as antiviral therapy.

The differences of clinical manifestations and laboratory findings in children and adults with dengue virus infection

2007 ◽  
Vol 39 (2) ◽  
pp. 76-81 ◽  
Author(s):  
Leera Kittigul ◽  
Piyamard Pitakarnjanakul ◽  
Dusit Sujirarat ◽  
Kanokrat Siripanichgon
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Dengue Virus Infection ◽  
Laboratory Findings

scholarly journals Frequency of Dengue virus Infection among Febrile Patients of Lahore

2017 ◽  
Vol 9 (10) ◽  
pp. 212
Author(s):  
M. Salman Munir Malik ◽  
Fahad Javed ◽  
Muhammad Wasim ◽  
Maham Ulfat ◽  
Sana Arshad ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Specific Antigen ◽  
Clinical Manifestations ◽  
P Value ◽  
Age Group ◽  
Dengue Virus Infection ◽  
Ns1 Antigen ◽  
Lahore City

BACKGROUND: Dengue virus infection is one of the major global public health problems. The infection usually occurs with clinical manifestations ranging from an asymptomatic or mild febrile illness as classical dengue fever to the potentially life-threatening illness, dengue hemorrhagic fever and dengue shock syndrome. The objective of the current study was to observe the frequency and diagnosis of primary or secondary dengue viral infection among individuals in Lahore city, Pakistan.METHODS: Study subjects were identified for the presence of dengue diagnostic markers including NS1 antigen, IgM and IgG antibody. The dengue specific antigen NS1 was detected by immonochromatography, while dengue specific antibodies (IgM and IgG) were measured through ELISA.RESULTS: Total 98 (56%) out of 175 febrile cases were found infected by dengue virus. From total 98 confirmed dengue cases, NS1 antigen was detected in 59 (60.20%), IgM antibodies were present in 74 (75.51%) and IgG antibodies were detected in 40 (40.81%) individuals. Statistical analysis reveals correlation of NS1 antigen and IgM antibody among dengue patients with significant P-value (P < 0.01). Results indicated that 58 (59%) were infected by primary infection and 40 (41%) were infected by secondary infection. The most effected age group was 21-30 years (51.02%) and least effected age group was <10 years (3.06%). Males were observed higher in number 61 (62%) as compare to females 37 (38%). Overall, the frequency of dengue virus infections were 56% among undifferentiated febrile patients in Lahore city, Pakistan.CONCLUSION: Dengue is found endemic in city population with increased incidence in monsoon and post monsoon. Constant vigilance of patients and dengue vector control awareness programs among public and health care officials could support in combating dengue.

scholarly journals NILAI DIAGNOSTIK ANTI DENGUE IgA DAN NS1, SERTA IgM/IgG DI INFEKSI VIRUS DENGUE (The Diagnostic Value of Anti Dengue IgA and Anti Dengue IgM/IgG in Dengue Virus Infection)

2018 ◽  
Vol 21 (1) ◽  
pp. 82
Author(s):  
Resna Resna ◽  
Aryati Aryati ◽  
Puspa Wardhani ◽  
Erwin Triyono
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Likelihood Ratio ◽  
Predictive Value ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Negative Likelihood Ratio ◽  
Diagnostic Value ◽  
Asia Pacific ◽  
Dengue Virus Infection

The clinical manifestations of dengue virus infection are varied and thus a specific diagnostic examination is required. Usually antidengueIgM is often used, but the presence in the circulation is 3−8 months long. NS1 is sensitive in the detection of primary infection,whereas IgG is more better used in secondary infection. The examination of anti-dengue IgA as a new marker is estimated to be ableto detect the acute primary and secondary infection, however the diagnostic value of anti-dengue IgA is not much well known for theIndonesian population. This study was done at the Tropical Infectious Disease Ward of Dr. Soetomo Hospital, Surabaya during February– April 2013. The samples consisted of 37 sera from patients infected by dengue virus and 37 sera from those non one (dengue virusinfection patients). The NS1 serum, anti-dengue IgM and anti dengue IgG were examined by ELISA and anti-dengue IgA was examined byan indirect immunochromatography method using Assure@ Dengue IgA Rapid Test (MP Biomedicals Asia Pacific Pte Ltd). The diagnosticvalue was analyzed by 2x2 table with a confidence interval of (CI) 95%. The used gold standards were from the 1997th WHO criteriaand one of the positive dengue serological tests by ELISA (NS1/anti dengue IgM/anti dengue IgG). AUC and anti-dengue IgA cut-off weredetermined by ROC curve. The Diagnostic value of anti-dengue IgA showed a sensitivity and specificity of 83.8% (67.3 to 93.2) and 81.1%(64.3 to 91.4). A positive predictive value of 81.6% (65.1 to 91.7) and a negative predictive value of 83.3% (66.5 to 93.0) was found. Thepositive likelihood ratio was 4.4 times (2.2 to 8.8) and negative likelihood ratio of only 0.2 times (0.09 to 0.42). The best cut off valueof 0.2 was shown by the area under the curve of 83.5%. Based on this study, the diagnostic value of anti-dengue IgA had a good validityfor the diagnosis of dengue virus infection.

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