scholarly journals Evaluation of Molecular Epidemiology, Clinical Characteristics, Antifungal Susceptibility Profiles, and Molecular Mechanisms of Antifungal Resistance of Iranian Candida parapsilosis Species Complex Blood Isolates

2020 ◽  
Vol 10 ◽  
Author(s):  
Amir Arastehfar ◽  
Farnaz Daneshnia ◽  
Mohammad Javad Najafzadeh ◽  
Ferry Hagen ◽  
Shahram Mahmoudi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Species Complex ◽  
Clinical Characteristics ◽  
Molecular Mechanisms ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Antifungal Resistance ◽  
Susceptibility Profiles

scholarly journals Identification of Candida parapsilosis Species Complex and Lodderomyces elongisporus Isolates with MALDI-TOF MS

2021 ◽  
Author(s):  
Engin Kaplan ◽  
Ayşe Sultan Karakoyun ◽  
Deniz Alkaya ◽  
Nevzat Ünal ◽  
Aylin Döğen ◽  
...  
Keyword(s):  
Species Complex ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Maldi Tof Ms ◽  
Biochemical Methods ◽  
Maldi Tof ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles ◽  
Reference Strains ◽  
Tof Ms

Objective: Candida parapsilosis species complex and Lodderomyces elongisporus may have differences in terms of their virulence, prevalence, and antifungal susceptibility profiles. These species are difficult to identify with biochemical methods. Therefore, there is a need for more efficient identification methods in terms of time, cost, and applicability. This study aims to evaluate the diagnostic performance of the MALDI-TOF MS method in discriminating between isolates belonging to the C. parapsilosis species complex and L. elongisporus. Method: In the current study, a total of 32 reference strains, including the C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6), and L. elongisporus (n=11) species were identified using the MALDI-TOF MS method. Results: The species names of 31 (93.7%) isolates belonging to the C. parapsilosis species complex and L.elongisporus were correctly identified. Twenty four isolates including eight (100%) C. parapsilosis, five (83%) C. metapsilosis, five (71%) C. orthopsilosis, and six (54%) L. elongisporus isolates were identified with score values ranging from 1.7 to 2.14. According to the secure identification reference score of ≥ 1.7, the sensitivity and specificity of the MALDI-TOF MS method were determined as 54.5–100% and 96.3–100%, respectively. Conclusion: Although the MALDI-TOF MS method has been shown to be effective in the rapid molecular phenotypic diagnosis of species that were difficult to discriminate using biochemical methods such as C. parapsilosis species complex and L. elongisporus, there is a clear need to optimize the method and develop a larger MS library for species-level identification within secure score ranges.

scholarly journals Molecular epidemiology and antifungal susceptibility profiles of clinical Cryptococcus neoformans/Cryptococcus gattii species complex

2020 ◽  
Vol 69 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Zainab Bandalizadeh ◽  
Tahereh Shokohi ◽  
Hamid Badali ◽  
Mahdi Abastabar ◽  
Farhang Babamahmoudi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Cryptococcus Neoformans ◽  
Species Complex ◽  
Antifungal Susceptibility ◽  
Cryptococcus Gattii ◽  
Susceptibility Profiles

Candidaemia due to Candida parapsilosis species complex at a hospital in Brazil: Clinical characteristics and antifungal susceptibility profile

2017 ◽  
Vol 34 (2) ◽  
pp. 106-108 ◽  
Author(s):  
Débora de Souza Olartechea de Alencar ◽  
Rosianne Assis de Sousa Tsujisaki ◽  
Fernanda Luiza Espinosa Spositto ◽  
Maína de Oliveira Nunes ◽  
Adriana Araújo de Almeida ◽  
...  
Keyword(s):  
Species Complex ◽  
Clinical Characteristics ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility

scholarly journals Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

2012 ◽  
Vol 61 (7) ◽  
pp. 1003-1008 ◽  
Author(s):  
Lucas Xavier Bonfietti ◽  
Marilena dos Anjos Martins ◽  
Maria Walderez Szeszs ◽  
Sandra Brasil Stolf Pukiskas ◽  
Sonia Ueda Purisco ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles

scholarly journals Antifungal susceptibility and virulence of Candida parapsilosis species complex: an overview of their pathogenic potential

2018 ◽  
Vol 67 (7) ◽  
pp. 903-914 ◽  
Author(s):  
Raimunda Sâmia Nogueira Brilhante ◽  
Jamille Alencar Sales ◽  
Maria Lucilene Queiroz da Silva ◽  
Jonathas Sales de Oliveira ◽  
Lucas de Alencar Pereira ◽  
...  
Keyword(s):  
Species Complex ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Pathogenic Potential

scholarly journals Candida parapsilosis: from Genes to the Bedside

2019 ◽  
Vol 32 (2) ◽  
Author(s):  
Renáta Tóth ◽  
Jozef Nosek ◽  
Héctor M. Mora-Montes ◽  
Toni Gabaldon ◽  
Joseph M. Bliss ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Candida Parapsilosis ◽  
Molecular Genetic ◽  
Antifungal Susceptibility ◽  
Resistance Mechanisms ◽  
Antifungal Drugs ◽  
Host Responses ◽  
Content Type ◽  
Antifungal Drug Resistance ◽  
Geographical Regions

SUMMARYPatients with suppressed immunity are at the highest risk for hospital-acquired infections. Among these, invasive candidiasis is the most prevalent systemic fungal nosocomial infection. Over recent decades, the combined prevalence of non-albicans Candidaspecies outrankedCandida albicansinfections in several geographical regions worldwide, highlighting the need to understand their pathobiology in order to develop effective treatment and to prevent future outbreaks.Candida parapsilosisis the second or third most frequently isolatedCandidaspecies from patients. Besides being highly prevalent, its biology differs markedly from that ofC. albicans, which may be associated withC. parapsilosis’ increased incidence. Differences in virulence, regulatory and antifungal drug resistance mechanisms, and the patient groups at risk indicate that conclusions drawn fromC. albicanspathobiology cannot be simply extrapolated toC. parapsilosis. Such species-specific characteristics may also influence their recognition and elimination by the host and the efficacy of antifungal drugs. Due to the availability of high-throughput, state-of-the-art experimental tools and molecular genetic methods adapted toC. parapsilosis, genome and transcriptome studies are now available that greatly contribute to our understanding of what makes this species a threat. In this review, we summarize 10 years of findings onC. parapsilosispathogenesis, including the species’ genetic properties, transcriptome studies, host responses, and molecular mechanisms of virulence. Antifungal susceptibility studies and clinician perspectives are discussed. We also present regional incidence reports in order to provide an updated worldwide epidemiology summary.

Prevalence and antifungal susceptibility profiles of Candida glabrata, Candida parapsilosis and their close-related species in oral candidiasis

2018 ◽  
Vol 95 ◽  
pp. 100-107 ◽  
Author(s):  
Katherine Miranda-Cadena ◽  
Cristina Marcos-Arias ◽  
Estibaliz Mateo ◽  
José Manuel Aguirre ◽  
Guillermo Quindós ◽  
...  
Keyword(s):  
Related Species ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Susceptibility Profiles
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