scholarly journals Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, and Candida in Human Blood Samples

Author(s):  
Flora Marzia Liotti ◽  
Brunella Posteraro ◽  
Franca Mannu ◽  
Franco Carta ◽  
Antonella Pantaleo ◽  
...  
Author(s):  
Andika Aliviameita ◽  
Meryn Linanda Agustin ◽  
Anggie Wedha Puspita ◽  
Miftahul Mushlih ◽  
Puspitasari Puspitasari ◽  
...  

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.


RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.


1988 ◽  
Vol 539 (1 Lyme Disease) ◽  
pp. 444-445 ◽  
Author(s):  
GUY BARANTON ◽  
ISABELLE SAINT-GIRONS

2015 ◽  
Vol 8 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Jasbir Singh Bedi ◽  
J. P. S. Gill ◽  
P. Kaur ◽  
A. Sharma ◽  
R. S. Aulakh

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