scholarly journals Molecular Identification, Genotypic Diversity, Antifungal Susceptibility, and Clinical Outcomes of Infections Caused by Clinically Underrated Yeasts, Candida orthopsilosis, and Candida metapsilosis: An Iranian Multicenter Study (2014–2019)

2019 ◽  
Vol 9 ◽  
Author(s):  
Amir Arastehfar ◽  
Sadegh Khodavaisy ◽  
Farnaz Daneshnia ◽  
Mohammad-Javad Najafzadeh ◽  
Shahram Mahmoudi ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Molecular Identification ◽  
Multicenter Study ◽  
Antifungal Susceptibility ◽  
Genotypic Diversity ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis

scholarly journals Prospective Multicenter Study of the Epidemiology, Molecular Identification, and Antifungal Susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis Isolated from Patients with Candidemia

2011 ◽  
Vol 55 (12) ◽  
pp. 5590-5596 ◽  
Author(s):  
Emilia Cantón ◽  
Javier Pemán ◽  
Guillermo Quindós ◽  
Elena Eraso ◽  
Ilargi Miranda-Zapico ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Multicenter Study ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Colorimetric Method ◽  
Prospective Multicenter Study ◽  
Content Type ◽  
Adult Intensive Care Unit ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis

ABSTRACTA 13-month prospective multicenter study including 44 hospitals was carried out to evaluate the epidemiology ofCandida parapsilosiscomplex candidemia in Spain. Susceptibility to amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, caspofungin, and micafungin was tested by the microdilution colorimetric method. A total of 364C. parapsilosiscomplex isolates were identified by molecular methods:C. parapsilosis(90.7%),Candida orthopsilosis(8.2%), andCandida metapsilosis(1.1%). Most candidemias (C. parapsilosis, 76.4%;C. orthopsilosis, 70.0%;C. metapsilosis, 100%) were observed in adults. NoC. orthopsilosisorC. metapsilosiscandidemias occurred in neonates.C. parapsilosiswas most frequent in adult intensive care unit (28.8%), surgery (20.9%), and internal medicine (19.7%) departments; andC. orthopsilosiswas most frequent in hematology (28.6%), pediatrics (12.0%), and neonatology (11.5%) departments. The geographic distribution ofC. orthopsilosisandC. metapsilosiswas not uniform. According to CLSI clinical breakpoints, allC. orthopsilosisandC. metapsilosisisolates were susceptible to the nine agents tested. Resistance (MICs > 1 mg/liter) was observed only inC. parapsilosis: amphotericin B, posaconazole, itraconazole, and caspofungin (0.3% each), anidulafungin (1.9%), and micafungin (2.5%). Applying the new species-specific fluconazole and echinocandin breakpoints, the rates of resistance to fluconazole forC. parapsilosisandC. orthopsilosisincreased to 4.8% and 0.3%, respectively; conversely, forC. parapsilosisthey shifted from 1.9 to 0.6% (anidulafungin) and from 2.5 to 0.6% (micafungin). Our study confirms the different prevalence ofC. parapsilosiscomplex candidemia among age groups: neitherC. orthopsilosisnorC. metapsilosiswas isolated from neonates; interestingly,C. metapsilosiswas isolated only from adults and the elderly. The disparity in antifungal susceptibility among species could be important for therapy.

scholarly journals Prevalence, distribution and antifungal susceptibility profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis bloodstream isolates

2012 ◽  
Vol 61 (7) ◽  
pp. 1003-1008 ◽  
Author(s):  
Lucas Xavier Bonfietti ◽  
Marilena dos Anjos Martins ◽  
Maria Walderez Szeszs ◽  
Sandra Brasil Stolf Pukiskas ◽  
Sonia Ueda Purisco ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Susceptibility Profiles

scholarly journals Molecular differentiation and antifungal susceptibilities of Candida parapsilosis isolated from patients with bloodstream infections

2009 ◽  
Vol 58 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Sun Tee Tay ◽  
Shiang Ling Na ◽  
Jennifer Chong
Keyword(s):  
Candida Parapsilosis ◽  
Susceptibility Testing ◽  
Rapd Analysis ◽  
Antifungal Susceptibility ◽  
Bloodstream Infections ◽  
Candida Metapsilosis ◽  
Significant Difference ◽  
Its Gene ◽  
Candida Orthopsilosis ◽  
Susceptibility Patterns

The genetic heterogeneity and antifungal susceptibility patterns of Candida parapsilosis isolated from blood cultures of patients were investigated in this study. Randomly amplified polymorphic DNA (RAPD) analysis generated 5 unique profiles from 42 isolates. Based on the major DNA fragments of the RAPD profiles, the isolates were identified as RAPD type P1 (29 isolates), P2 (6 isolates), P3 (4 isolates), P4 (2 isolates) and P5 (1 isolate). Sequence analysis of the internal transcribed spacer (ITS) gene of the isolates identified RAPD type P1 as C. parapsilosis, P2 and P3 as Candida orthopsilosis, P4 as Candida metapsilosis, and P5 as Lodderomyces elongisporus. Nucleotide variations in ITS gene sequences of C. orthopsilosis and C. metapsilosis were detected. Antifungal susceptibility testing using Etests showed that all isolates tested in this study were susceptible to amphotericin B, fluconazole, ketoconazole, itraconazole and voriconazole. C. parapsilosis isolates exhibited higher MIC50 values than those of C. orthopsilosis for all of the drugs tested in this study; however, no significant difference in the MICs for these two Candida species was observed. The fact that C. orthopsilosis and C. metapsilosis were responsible for 23.8 and 4.8 % of the cases attributed to C. parapsilosis bloodstream infections, respectively, indicates the clinical relevance of these newly described yeasts. Further investigations of the ecological niche, mode of transmission and virulence of these species are thus essential.

scholarly journals Microscopic Evaluation, Molecular Identification, Antifungal Susceptibility, and Clinical Outcomes inFusarium,Aspergillusand, Dematiaceous Keratitis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Devarshi U. Gajjar ◽  
Anuradha K. Pal ◽  
Bharat K. Ghodadra ◽  
Abhay R. Vasavada
Keyword(s):  
Clinical Outcomes ◽  
Amphotericin B ◽  
Molecular Identification ◽  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Its Region ◽  
Fungal Species ◽  
Fungal Keratitis ◽  
Accurate Identification ◽  
Microscopic Evaluation

Purpose.Fusarium,Aspergillus, and Dematiaceous are the most common fungal species causing keratitis in tropical countries. Herein we report a prospective study on fungal keratitis caused by these three fungal species.Methodology. A prospective investigation was undertaken to evaluate eyes with presumed fungal keratitis. All the fungal isolates (n=73) obtained from keratitis infections were identified using morphological and microscopic characters. Molecular identification using sequencing of the ITS region and antifungal susceptibility tests using microdilution method were done. The final clinical outcome was evaluated in terms of the time taken for resolution of keratitis and the final visual outcome. The results were analyzed after segregating the cases into three groups, namely,Fusarium,Aspergillus, and Dematiaceous keratitis.Results. Diagnosis of fungal keratitis was established in 73 (35.9%) cases out of 208 cases. The spectra of fungi isolated wereFusariumspp. (26.6%),Aspergillusspp. (21.6%), and Dematiaceous fungi (11.6%). The sequence of the ITS region could identify theFusariumandAspergillusspecies at the species complex level, and the Dematiaceous isolates were accurately identified. Using antifungal agents such as fluconazole, natamycin, amphotericin B, and itraconazole, the minimum inhibitory concentrations (MICs) forFusariumspp. were >32 μg/mL, 4–8 μg/mL, 0.5–1 μg/mL, and >32 μg/mL, respectively. Antifungal susceptibility data showed thatCurvulariaspp. was highly resistant to all the antifungal agents. Overall, natamycin and amphotericin B were found to be the most effective antifungal agents. The comparative clinical outcomes in all cases showed that the healing response in terms of visual acuity of the Dematiaceous group was significantly good when compared with theFusariumandAspergillusgroups (P<0.05). The time required for healing in theFusariumgroup was statistically significantly less when compared with theAspergillusand Dematiaceous groups.Conclusion. This study demonstrates important differences in microscopic features of scraping material and antifungal susceptibility between the three groups. Early and accurate identification coupled with the MIC data, and thereby appropriate treatment is crucial for complete recovery.

scholarly journals A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Penghao Guo ◽  
Yuting He ◽  
Rui Fan ◽  
Zhongwen Wu ◽  
Yili Chen ◽  
...  
Keyword(s):  
Prosthetic Valve ◽  
Candida Parapsilosis ◽  
Case Series ◽  
Prosthetic Valve Endocarditis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

Three is a crowd: Clinical outcomes of a twice daily versus a thrice daily metronidazole dosing strategy from a multicenter study

Anaerobe ◽  
2021 ◽  
pp. 102378
Author(s):  
Sunish Shah ◽  
Kathleen Adams ◽  
Jacob Merwede ◽  
Dayna McManus ◽  
Jeffrey Topal
Keyword(s):  
Clinical Outcomes ◽  
Multicenter Study ◽  
Dosing Strategy
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