scholarly journals BfvR, an AraC-Family Regulator, Controls Biofilm Formation and pH6 Antigen Production in Opposite Ways in Yersinia pestis Biovar Microtus

2018 ◽  
Vol 8 ◽  
Author(s):  
Haihong Fang ◽  
Lei Liu ◽  
Yiquan Zhang ◽  
Huiying Yang ◽  
Yanfeng Yan ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation ◽  
Antigen Production ◽  
Arac Family

scholarly journals Identification of gmhA, a Yersinia pestis Gene Required for Flea Blockage, by Using a Caenorhabditis elegans Biofilm System

2005 ◽  
Vol 73 (11) ◽  
pp. 7236-7242 ◽  
Author(s):  
Creg Darby ◽  
Sandya L. Ananth ◽  
Li Tan ◽  
B. Joseph Hinnebusch
Keyword(s):  
Caenorhabditis Elegans ◽  
Yersinia Pestis ◽  
Biofilm Formation ◽  
Yersinia Pseudotuberculosis ◽  
C Elegans ◽  
Transposon Insertion ◽  
Insertion Mutants ◽  
Random Screening

ABSTRACT Yersinia pestis, the cause of bubonic plague, blocks feeding by its vector, the flea. Recent evidence indicates that blockage is mediated by an in vivo biofilm. Y. pestis and the closely related Yersinia pseudotuberculosis also make biofilms on the cuticle of the nematode Caenorhabditis elegans, which block this laboratory animal's feeding. Random screening of Y. pseudotuberculosis transposon insertion mutants with a C. elegans biofilm assay identified gmhA as a gene required for normal biofilms. gmhA encodes phosphoheptose isomerase, an enzyme required for synthesis of heptose, a conserved component of lipopolysaccharide and lipooligosaccharide. A Y. pestis gmhA mutant was constructed and was severely defective for C. elegans biofilm formation and for flea blockage but only moderately defective in an in vitro biofilm assay. These results validate use of the C. elegans biofilm system to identify genes and pathways involved in Y. pestis flea blockage.

scholarly journals Poly-N-Acetylglucosamine Expression by Wild-Type Yersinia pestis Is Maximal at Mammalian, Not Flea, Temperatures

mBio ◽  
2012 ◽  
Vol 3 (4) ◽  
Author(s):  
Pauline Yoong ◽  
Colette Cywes-Bentley ◽  
Gerald B. Pier
Keyword(s):  
Yersinia Pestis ◽  
Congo Red ◽  
Biofilm Formation ◽  
Mammalian Host ◽  
Virulence Plasmid ◽  
Insect Vector ◽  
Wild Type ◽  
Content Type ◽  
Higher Temperature ◽  
Mammalian Infection

ABSTRACTNumerous bacteria, includingYersinia pestis, express the poly-N-acetylglucosamine (PNAG) surface carbohydrate, a major component of biofilms often associated with a specific appearance of colonies on Congo red agar. Biofilm formation and PNAG synthesis byY. pestishave been reported to be maximal at 21 to 28°C or “flea temperatures,” facilitating the regurgitation ofY. pestisinto a mammalian host during feeding, but production is diminished at 37°C and thus presumed to be decreased during mammalian infection. Most studies of PNAG expression and biofilm formation byY. pestishave used a low-virulence derivative of strain KIM, designated KIM6+, that lacks the pCD1 virulence plasmid, and an isogenic mutant without the pigmentation locus, which contains the hemin storage genes that encode PNAG biosynthetic proteins. Using confocal microscopy, fluorescence-activated cell sorter analysis and growth on Congo red agar, we confirmed prior findings regarding PNAG production with the KIM6+ strain. However, we found that fully virulent wild-type (WT) strains KIM and CO92 had maximal PNAG expression at 37°C, with lower PNAG production at 28°C both in broth medium and on Congo red agar plates. Notably, the typical dark colony morphology appearing on Congo red agar was maintained at 28°C, indicating that this phenotype is not associated with PNAG expression in WTY. pestis. Extracts of WT sylvaticY. pestisstrains from the Russian Federation confirmed the maximal expression of PNAG at 37°C. PNAG production by WTY. pestisis maximal at mammalian and not insect vector temperatures, suggesting that this factor may have a role during mammalian infection.IMPORTANCEYersinia pestistransitions from low-temperature residence and replication in insect vectors to higher-temperature replication in mammalian hosts. Prior findings based primarily on an avirulent derivative of WT (wild-type) KIM, named KIM6+, showed that biofilm formation associated with synthesis of poly-N-acetylglucosamine (PNAG) is maximal at 21 to 28°C and decreased at 37°C. Biofilm formation was purported to facilitate the transmission ofY. pestisfrom fleas to mammals while having little importance in mammalian infection. Here we found that for WT strains KIM and CO92, maximal PNAG production occurs at 37°C, indicating that temperature regulation of PNAG production in WTY. pestisis not mimicked by strain KIM6+. Additionally, we found that Congo red binding does not always correlate with PNAG production, despite its widespread use as an indicator of biofilm production. Taken together, the findings show that a role for PNAG in WTY. pestisinfection should not be disregarded and warrants further study.

HmsP, a putative phosphodiesterase, and HmsT, a putative diguanylate cyclase, control Hms-dependent biofilm formation in Yersinia pestis

2004 ◽  
Vol 54 (1) ◽  
pp. 75-88 ◽  
Author(s):  
Olga Kirillina ◽  
Jacqueline D. Fetherston ◽  
Alexander G. Bobrov ◽  
Jennifer Abney ◽  
Robert D. Perry
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation ◽  
Diguanylate Cyclase

scholarly journals Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Zizhong Liu ◽  
Xiaofang Gao ◽  
Hongduo Wang ◽  
Haihong Fang ◽  
Yanfeng Yan ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation

scholarly journals Differential Control of Yersinia pestis Biofilm Formation In Vitro and in the Flea Vector by Two c-di-GMP Diguanylate Cyclases

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e19267 ◽  
Author(s):  
Yi-Cheng Sun ◽  
Alexandra Koumoutsi ◽  
Clayton Jarrett ◽  
Kevin Lawrence ◽  
Frank C. Gherardini ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation ◽  
Diguanylate Cyclases ◽  
Differential Control

scholarly journals Fur Is a Repressor of Biofilm Formation in Yersinia pestis

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52392 ◽  
Author(s):  
Fengjun Sun ◽  
He Gao ◽  
Yiquan Zhang ◽  
Li Wang ◽  
Nan Fang ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation

scholarly journals HmsP, a putative phosphodiesterase, and HmsT, a putative diguanylate cyclase, control Hms-dependent biofilm formation in Yersinia pestis

2004 ◽  
Vol 54 (4) ◽  
pp. 1134-1134
Author(s):  
Olga Kirillina ◽  
Jacqueline D. Fetherston ◽  
Alexander G. Bobrov ◽  
Jennifer Abney ◽  
Robert D. Perry
Keyword(s):  
Yersinia Pestis ◽  
Biofilm Formation ◽  
Diguanylate Cyclase
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