scholarly journals Autoregulation of ToxR and Its Regulatory Actions on Major Virulence Gene Loci in Vibrio parahaemolyticus

2018 ◽  
Vol 8 ◽  
Author(s):  
Yiquan Zhang ◽  
Lingfei Hu ◽  
George Osei-Adjei ◽  
Ying Zhang ◽  
Wenhui Yang ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Virulence Gene ◽  
Regulatory Actions

scholarly journals Serogroup, Virulence, and Genetic Traits of Vibrio parahaemolyticus in the Estuarine Ecosystem of Bangladesh

2009 ◽  
Vol 75 (19) ◽  
pp. 6268-6274 ◽  
Author(s):  
Munirul Alam ◽  
Wasimul B. Chowdhury ◽  
N. A. Bhuiyan ◽  
Atiqul Islam ◽  
Nur A. Hasan ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Marker Gene ◽  
Human Health Risk ◽  
Geographic Origin ◽  
Virulence Gene ◽  
Estuarine Ecosystem ◽  
Genetic Traits ◽  
Clinical Strains ◽  
Pandemic Strains ◽  
And Cluster Analysis

ABSTRACT Forty-two strains of Vibrio parahaemolyticus were isolated from Bay of Bengal estuaries and, with two clinical strains, analyzed for virulence, phenotypic, and molecular traits. Serological analysis indicated O8, O3, O1, and K21 to be the major O and K serogroups, respectively, and O8:K21, O1:KUT, and O3:KUT to be predominant. The K antigen(s) was untypeable, and pandemic serogroup O3:K6 was not detected. The presence of genes tox R and tlh were confirmed by PCR in all but two strains, which also lacked tox R. A total of 18 (41%) strains possessed the virulence gene encoding thermostable direct hemolysin (TDH), and one had the TDH-related hemolysin (trh) gene, but not tdh. Ten (23%) strains exhibited Kanagawa phenomenon that surrogates virulence, of which six, including the two clinical strains, possessed tdh. Of the 18 tdh-positive strains, 17 (94%), including the two clinical strains, had the seromarker O8:K21, one was O9:KUT, and the single trh-positive strain was O1:KUT. None had the group-specific or ORF8 pandemic marker gene. DNA fingerprinting employing pulsed-field gel electrophoresis (PFGE) of SfiI-digested DNA and cluster analysis showed divergence among the strains. Dendrograms constructed using PFGE (SfiI) images from a soft database, including those of pandemic and nonpandemic strains of diverse geographic origin, however, showed that local strains formed a cluster, i.e., “clonal cluster,” as did pandemic strains of diverse origin. The demonstrated prevalence of tdh-positive and diarrheagenic serogroup O8:K21 strains in coastal villages of Bangladesh indicates a significant human health risk for inhabitants.

scholarly journals Virulence gene, antimicrobial resistance and phylogenetic characterization of Vibrio parahaemolyticus in migratory birds, Guangdong

2020 ◽  
Author(s):  
Lin zheng ◽  
Ping Chen ◽  
Jun Xue Guo ◽  
Ling Wei Zhu ◽  
Yao Jia Guan ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Resistance Genes ◽  
Vibrio Parahaemolyticus ◽  
Migratory Birds ◽  
Virulence Gene ◽  
Migratory Bird ◽  
Phylogenetic Characterization ◽  
Contaminated Food ◽  
Intestinal Pathogen ◽  
Drug Resistant Strains

Abstract Background Vibrio parahaemolyticus is an intestinal pathogen that is transmitted by contaminated aquatic products.Migratory birds ate foods infected with Vibrio parahaemolyticus, excreting feces on other foods. Other organisms ate contaminated food again, leading to the spread of strains. It were reported previously that Vibrio parahaemolyticus was sensitive to most antibiotics except penicillin such as ampicillin and amoxicillin. However, due to the frequent used of antibiotics in recent years, Vibrio parahaemolyticus developed resistance to antibiotics. The recommended antibiotics for Vibrio parahaemolyticus treatment were tetracycline, β-lactam, quinolone and sulfonamide. In the storage migratory bird, Vibrio parahaemolyticus could complete the accumulation of drug resistance, resulting in the generation of drug resistance genes, or the horizontal drug resistance genes transfer from other bacteria, resulting obtain drug resistance genes. Results In this study, 122 isolates of Vibrio parahaemolyticus were obtained for further pathogenicity identification, antibacterial susceptibility testing of 18 antimicrobial compounds and genetic characterization based on the whole genome sequencing. There is no pandemic serotype isolated between migratory birds, but 37 strains with cholera toxin. Five strains had col3M plasmid-moderately resistant drug-resistant strains of quinolone mediated by the qnrD1 gene. For the first time in China, Vibrio parahaemolyticus with col3M plasmid-mediated qnrD1 gene was isolated from migratory birds. Conclusion According to the mutltilocus sequence typing analysis, authors found the bacterial genetic diversity is higher, the clones are less and there is regional aggregation in Shenzhen. And mallards are more susceptible to Vibrio parahaemolyticus.

Differences in the Stress Tolerances of Vibrio parahaemolyticus Strains due to Their Source and Harboring of Virulence Genes

2013 ◽  
Vol 76 (8) ◽  
pp. 1456-1462 ◽  
Author(s):  
AKIO HASEGAWA ◽  
YUKIKO HARA-KUDO ◽  
KIKUYO OGATA ◽  
SHIOKO SAITO ◽  
YOSHIKO SUGITA-KONISHI ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Vibrio Parahaemolyticus ◽  
Virulence Genes ◽  
Acid Stress ◽  
Virulence Gene ◽  
Coastal Environment ◽  
Coastal Environments ◽  
Coastal Seawater ◽  
Virulent Strains ◽  
Foodborne Infection

To investigate the diversity of stress tolerance levels in Vibrio parahaemolyticus, 200 V. parahaemolyticus strains isolated from various coastal environments, seafood, and human clinical cases were exposed to acid, low-osmolality, freezing-thawing, and heat stresses. Tolerance against acid stress was higher in the virulent (tdh- and/or trh-positive) strains than in the avirulent (tdh- and trh-negative) strains. Tolerance against low-osmolality, freezing-thawing, and heat stresses was higher in the clinical strains of tdh- and/or trh-positive V. parahaemolyticus than in the coastal environment– and seafood-originated strains of tdhand/or trh-positive V. parahaemolyticus. Tolerance against acid stress was higher in the strains isolated from coastal seawater at ≤15°C than in the strains isolated at ≥20°C. Tolerance against heat stress was higher in the avirulent strains than the virulent strains, and in the strains isolated from coastal seawater at ≥20°C than the strains isolated from coastal seawater at ≤15°C. Therefore, this study demonstrated that the diversity of stress tolerance levels in V. parahaemolyticus strains depended on their source and whether they harbored virulence genes. In particular, there was significantly greater tolerance against acid in the virulence gene–harboring strains and strains isolated from low-temperature seawater. Because the stress tolerances of V. parahaemolyticus have direct influences for the survival in environment and food, it is important for the prevention of foodborne infection to control the stress-tolerant strains.

Pandemic strains of O3:K6Vibrio parahaemolyticusin the aquatic environment of Bangladesh

2004 ◽  
Vol 50 (10) ◽  
pp. 827-834 ◽  
Author(s):  
M Sirajul Islam ◽  
Rizwana Tasmin ◽  
Sirajul Islam Khan ◽  
Habibul Bari Mahmud Bakht ◽  
Zahid Hayat Mahmood ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Vibrio Parahaemolyticus ◽  
Blot Hybridization ◽  
Virulence Gene ◽  
Gene Probe ◽  
Chromosomal Dna ◽  
Specific Pcr ◽  
Pandemic Strains ◽  
Positive Results ◽  
Tdh Gene

A total of 1500 environmental strains of Vibrio parahaemolyticus, isolated from the aquatic environment of Bangladesh, were screened for the presence of a major V. parahaemolyticus virulence factor, the thermostable direct haemolysin (tdh) gene, by the colony blot hybridization method using a digoxigenin-labeled tdh gene probe. Of 1500 strains, 5 carried the tdh sequence, which was further confirmed by PCR using primers specific for the tdh gene. Examination by PCR confirmed that the 5 strains were V. parahamolyticus and lacked the thermostable direct haemolysin-related haemolysin (trh) gene, the alternative major virulence gene known to be absent in pandemic strains. All 5 strains gave positive Kanagawa phenomenon reaction with characteristic β-haemolysis on Wagatsuma agar medium. Southern blot analysis of the HindIII-digested chromosomal DNA demonstrated, in all 5 strains, the presence of 2 tdh genes common to strains positive for Kanagawa phenomenon. However, the 5 strains were found to belong to 3 different serotypes (O3:K29, O4:K37, and O3:K6). The 2 with pandemic serotype O3:K6 gave positive results in group-specific PCR and ORF8 PCR assays, characteristics unique to the pandemic clone. Clonal variations among the 5 isolates were analyzed by comparing RAPD and ribotyping patterns. Results showed different patterns for the 3 serotypes, but the pattern was identical among the O3:K6 strains. This is the first report on the isolation of pandemic O3:K6 strains of V. parahaemolyticus from the aquatic environment of Bangladesh.Key words: pandemic strains, Vibrio parahaemolyticus, aquatic environment.

scholarly journals Regulatory actions of ToxR and CalR on their own genes and type III secretion system 1 in Vibrio parahaemolyticus

Oncotarget ◽  
2017 ◽  
Vol 8 (39) ◽  
pp. 65809-65822 ◽  
Author(s):  
George Osei-Adjei ◽  
He Gao ◽  
Ying Zhang ◽  
Lingyu Zhang ◽  
Wenhui Yang ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
System 1 ◽  
Regulatory Actions

scholarly journals Characterization of Pathogenic Vibrio parahaemolyticus Isolated From Fish Aquaculture of the Southwest Coastal Area of Bangladesh

2021 ◽  
Vol 12 ◽  
Author(s):  
Abu Baker Siddique ◽  
M. Moniruzzaman ◽  
Sobur Ali ◽  
Md. Nayem Dewan ◽  
Mohammad Rafiqul Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pearson Correlation ◽  
Penaeus Monodon ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Economic Losses ◽  
Ileal Loop ◽  
Virulence Potential ◽  
Pathogenic Vibrio

Vibrio parahaemolyticus is a major foodborne pathogen responsible for significant economic losses in aquaculture and a threat to human health. Here, we explored the incidence, virulence potential, and diversity of V. parahaemolyticus isolates from aquaculture farms in Bangladesh. We examined a total of 216 water, sediment, Oreochromis niloticus (tilapia), Labeo rohita (rui), and Penaeus monodon (shrimp) samples from the aquaculture system where 60.2% (130/216) samples were positive for V. parahaemolyticus. Furthermore, we identified 323 V. parahaemolyticus strains from contaminated samples, 17 of which were found positive for trh, a virulence gene. Four isolates out of the 17 obtained were able to accumulate fluid in the rabbit ileal loop assay. The correlation between the contamination of V. parahaemolyticus and environmental factors was determined by Pearson correlation. The temperature and salinity were significantly correlated (positive) with the incidence of V. parahaemolyticus. Most of the pathogenic isolates (94.1%) were found resistant to ampicillin and amoxicillin. O8: KUT was the predominant serotype of the potentially pathogenic isolates. ERIC-PCR reveals genetic variation and relatedness among the pathogenic isolates. Therefore, this region-specific study establishes the incidence of potential infection with V. parahaemolyticus from the consumption of tilapia, rui, and shrimp raised in farms in Satkhira, Bangladesh, and the basis for developing strategies to reduce the risk for diseases and economic burden.

scholarly journals Prevalence, characterization and antibiotic susceptibility of Vibrio parahaemolyticus isolated from fishes and shellfishes of coastal regions of Bangladesh

2015 ◽  
Vol 24 (2) ◽  
pp. 121-129
Author(s):  
Md Manirul Haque ◽  
Anwar Hossain ◽  
Shankar Chandra Mandal ◽  
Mohammad Shamsur Rahman ◽  
Zahid Hayat Mahmud
Keyword(s):  
Antibiotic Susceptibility ◽  
Vibrio Parahaemolyticus ◽  
Health Hazard ◽  
Virulence Gene ◽  
Specific Gene ◽  
Coastal Regions ◽  
Gene Encoding ◽  
Species Specific ◽  
Fish And Shellfish ◽  
Fish Industry

Occurrence of Vibrio parahaemolyticus in fishes and shellfishes of coastal regions of Bangladesh was investigated. Fish and shellfish samples were collected from three coastal areas, namely Kuakata, Chittagong and Cox’s Bazar. Thirty five V. parahaemolyticus strains were isolated from 33 finfish and 6 shellfish samples where all the isolates were tlh positive which was species specific gene and no isolate had possessed the virulence gene encoding tdh. Overall prevalence rate of V. parahaemolyticus in fish sample was 45.45%; having 18.75% from Kuakata, 22.22% from Chittagong and 62.5% from Cox’s Bazar. Fifty per cent shellfish were found to be positive for V. parahaemolyticus. Antibiotic susceptibility of the isolated strains was carried out against 11 antibiotics where the isolates were sensitive to the tested antibiotics except metronidazole (50 μg) and nalidixic acid (30 μg). Presence of this pathogenic organism in fish and shellfish could pose a serious threat to fish industry as well as human health hazard in Bangladesh. Dhaka Univ. J. Biol. Sci. 24(2): 121-129, 2015 (July)

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