scholarly journals microRNA-4516 Contributes to Different Functions of Epithelial Permeability Barrier by Targeting Poliovirus Receptor Related Protein 1 in Enterovirus 71 and Coxsackievirus A16 Infections

2018 ◽  
Vol 8 ◽  
Author(s):  
Yajie Hu ◽  
Jie Song ◽  
Longding Liu ◽  
Ying Zhang ◽  
Lichun Wang ◽  
...  
Keyword(s):  
Enterovirus 71 ◽  
Permeability Barrier ◽  
Epithelial Permeability ◽  
Related Protein ◽  
Coxsackievirus A16 ◽  
Poliovirus Receptor

scholarly journals VP2 Dominated CD4+T Cell Responses against Enterovirus 71 and Cross-Reactivity against Coxsackievirus A16 and Polioviruses in a Healthy Population

2013 ◽  
Vol 191 (4) ◽  
pp. 1637-1647 ◽  
Author(s):  
Shuguang Tan ◽  
Xiaojuan Tan ◽  
Xiaoman Sun ◽  
Guangwen Lu ◽  
Chun-Chi Chen ◽  
...  
Keyword(s):  
T Cell ◽  
Enterovirus 71 ◽  
T Cell Responses ◽  
Cross Reactivity ◽  
Cd4 T Cell ◽  
Healthy Population ◽  
Coxsackievirus A16 ◽  
Cell Responses

scholarly journals Eupafolin and Ethyl Acetate Fraction ofKalanchoe gracilisStem Extract Show Potent Antiviral Activities against Enterovirus 71 and Coxsackievirus A16

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ching-Ying Wang ◽  
Shun-Chueh Huang ◽  
Zhen-Rung Lai ◽  
Yu-Ling Ho ◽  
Yu-Jen Jou ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Folk Medicine ◽  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Ethyl Acetate Fraction ◽  
Coxsackievirus A16 ◽  
Aqueous Fraction ◽  
Antiviral Activities ◽  
Clinical Potential

Enterovirus 71 (EV71) and coxsackievirus A16 (CoxA16) are main pathogens of hand-foot-and-mouth disease, occasionally causing aseptic meningitis and encephalitis in tropical and subtropical regions.Kalanchoe gracilis,Da-Huan-Hun, is a Chinese folk medicine for treating pain and inflammation, exhibiting antioxidant and anti-inflammatory activities. Our prior report (2012) citedK. gracilisleaf extract as moderately active against EV71 and CoxA16. This study further rates antienteroviral potential ofK. gracilisstem (KGS) extract to identify potent antiviral fractions and components. The extract moderately inhibits viral cytopathicity and virus yield, as well asin vitroreplication of EV71 (IC50= 75.18 μg/mL) and CoxA16 (IC50= 81.41 μg/mL). Ethyl acetate (EA) fraction of KGS extract showed greater antiviral activity than that ofn-butanol or aqueous fraction: IC50values of 4.21 μg/mL against EV71 and 9.08 μg/mL against CoxA16. HPLC analysis, UV-Vis absorption spectroscopy, and plaque reduction assay indicate that eupafolin is a vital component of EA fraction showing potent activity against EV71 (IC50= 1.39 μM) and CoxA16 (IC50= 5.24 μM). Eupafolin specifically lessened virus-induced upregulation of IL-6 and RANTES by inhibiting virus-induced ERK1/2, AP-1, and STAT3 signals. Anti-enteroviral potency of KGS EA fraction and eupafolin shows the clinical potential against EV71 and CoxA16 infection.

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