scholarly journals PilY1 Promotes Legionella pneumophila Infection of Human Lung Tissue Explants and Contributes to Bacterial Adhesion, Host Cell Invasion, and Twitching Motility

2017 ◽  
Vol 7 ◽  
Author(s):  
Julia Hoppe ◽  
Can M. Ünal ◽  
Stefanie Thiem ◽  
Louisa Grimpe ◽  
Torsten Goldmann ◽  
...  
Keyword(s):  
Host Cell ◽  
Lung Tissue ◽  
Cell Invasion ◽  
Legionella Pneumophila ◽  
Bacterial Adhesion ◽  
Human Lung ◽  
Host Cell Invasion ◽  
Twitching Motility ◽  
Human Lung Tissue ◽  
Tissue Explants

scholarly journals Human Lung Tissue Explants Reveal Novel Interactions during Legionella pneumophila Infections

2013 ◽  
Vol 82 (1) ◽  
pp. 275-285 ◽  
Author(s):  
Jens Jäger ◽  
Sebastian Marwitz ◽  
Jana Tiefenau ◽  
Janine Rasch ◽  
Olga Shevchuk ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Legionella Pneumophila ◽  
Human Lung ◽  
Transcriptional Response ◽  
Human Infection ◽  
Bacterial Invasion ◽  
Human Lung Tissue ◽  
Content Type ◽  
Tissue Explants ◽  
Legionnaires Disease

ABSTRACTHistological and clinical investigations describe late stages of Legionnaires' disease but cannot characterize early events of human infection. Cellular or rodent infection models lack the complexity of tissue or have nonhuman backgrounds. Therefore, we developed and applied a novel model forLegionella pneumophilainfection comprising living human lung tissue. We stimulated lung explants withL. pneumophilastrains and outer membrane vesicles (OMVs) to analyze tissue damage, bacterial replication, and localization as well as the transcriptional response of infected tissue. Interestingly, we found that extracellular adhesion ofL. pneumophilato the entire alveolar lining precedes bacterial invasion and replication in recruited macrophages. In contrast, OMVs predominantly bound to alveolar macrophages. Specific damage to septa and epithelia increased over 48 h and was stronger in wild-type-infected and OMV-treated samples than in samples infected with the replication-deficient, type IVB secretion-deficient DotA−strain. Transcriptome analysis of lung tissue explants revealed a differential regulation of 2,499 genes after infection. The transcriptional response included the upregulation of uteroglobin and the downregulation of the macrophage receptor with collagenous structure (MARCO). Immunohistochemistry confirmed the downregulation of MARCO at sites of pathogen-induced tissue destruction. Neither host factor has ever been described in the context ofL. pneumophilainfections. This work demonstrates that the tissue explant model reproduces realistic features of Legionnaires' disease and reveals new functions for bacterial OMVs during infection. Our model allows us to characterize early steps of human infection which otherwise are not feasible for investigations.

Gene and protein responses of human lung tissue explants exposed to ambient particulate matter of different sizes

2012 ◽  
Vol 24 (14) ◽  
pp. 966-975 ◽  
Author(s):  
Bastiaan Hoogendoorn ◽  
Kelly Berube ◽  
Clive Gregory ◽  
Tim Jones ◽  
Keith Sexton ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Lung Tissue ◽  
Human Lung ◽  
Ambient Particulate Matter ◽  
Human Lung Tissue ◽  
Tissue Explants

scholarly journals Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Ying Chang ◽  
Laila Al-Alwan ◽  
Sama Alshakfa ◽  
Severine Audusseau ◽  
Andrea Karen Mogas ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Lung Tissue ◽  
Human Lung ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Human Lung Tissue ◽  
Tissue Explants

scholarly journals Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Julia A. Horstmann ◽  
Michele Lunelli ◽  
Hélène Cazzola ◽  
Johannes Heidemann ◽  
Caroline Kühne ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Host Cell ◽  
Cell Invasion ◽  
Bacterial Adhesion ◽  
Host Cell Invasion

Validation Of Anti-Host Cell Influenza Targets Using A Novel Human Lung Tissue Model

2012 ◽  
Author(s):  
Ben Nicholas ◽  
Karl J. Staples ◽  
Stefan Moese ◽  
Jon Ward ◽  
Malcolm C. North ◽  
...  
Keyword(s):  
Host Cell ◽  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue ◽  
Tissue Model
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