scholarly journals Genome-Wide Transcriptional and Post-transcriptional Regulation of Innate Immune and Defense Responses of Bovine Mammary Gland to Staphylococcus aureus

2016 ◽  
Vol 6 ◽  
Author(s):  
Lingzhao Fang ◽  
Yali Hou ◽  
Jing An ◽  
Bingjie Li ◽  
Minyan Song ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Transcriptional Regulation ◽  
Mammary Gland ◽  
Defense Responses ◽  
Innate Immune ◽  
Bovine Mammary Gland ◽  
Genome Wide ◽  
Post Transcriptional Regulation

Genome-wide analysis of mammary gland shows modulation of transcriptome landscape with alternative splice variants in Staphylococcus aureus mastitis in mice

Gene ◽  
2020 ◽  
Vol 735 ◽  
pp. 144278
Author(s):  
Susweta Das Mitra ◽  
Feroze Ganaie ◽  
Kiran Bankar ◽  
Dhanikachalam Velu ◽  
Bhuvana Mani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Alternative Splice ◽  
Splice Variants ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Alternative Splice Variants

Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis

2007 ◽  
Vol 54 (4) ◽  
pp. 399-409 ◽  
Author(s):  
Javier Oviedo-Boyso ◽  
Juan J. Valdez-Alarcón ◽  
Marcos Cajero-Juárez ◽  
Alejandra Ochoa-Zarzosa ◽  
Joel E. López-Meza ◽  
...  
Keyword(s):  
Immune Response ◽  
Mammary Gland ◽  
Innate Immune Response ◽  
Pathogenic Bacteria ◽  
Innate Immune ◽  
Bovine Mammary Gland

Phagocytosis of Staphylococcus aureus by bovine mammary gland macrophages and intracellular protection from antibiotic action in vitro and in vivo

1984 ◽  
Vol 51 (4) ◽  
pp. 513-523 ◽  
Author(s):  
Neil Craven ◽  
James C. Anderson
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Antibiotic Therapy ◽  
Bovine Mammary Gland ◽  
Lactating Cows ◽  
Colony Forming Units ◽  
Subsequent Exposure ◽  
Increased Sensitivity

SummaryMacrophages isolated from the involuted bovine mammary gland were cultured in vitro. Phagocytosis of opsonized Staphylococcus aureus occurred rapidly, but intracellular killing of bacteria was slow. Many intracellular staphylococci survived for up to 4 d exposure to extracellular cloxacillin and emerged from within the macrophages to multiply extracellularly when the antibiotic was inactivated. Rifampicin was significantly more efficient than cloxacillin in killing intracellular S. aureus after 18 h incubation, but it too failed to sterilize the cultures within 3 d. Staphylococci, which had remained viable within macrophages during 20 h incubation with extracellular cloxacillin, showed an increased sensitivity to dilute lysostaphin on subsequent exposure. A 3 d course of intramammary therapy with cloxacillin, commencing simultaneously with an infecting inoculum of ∼108 colony forming units (c.f.u.) S. aureus, apparently eliminated the infection from one quarter of the udders of each of three lactating cows, but bacteria were re-isolated from two cows after a delay of several days. However, when other quarters of the same cows were infected with ∼108 c.f.u. S. aureus which had been phagocytosed by autologous mammary macrophages, similar simultaneous antibiotic therapy failed to affect these infections. The in vitro and in vivo findings indicate the significance of intracellular survival of S. aureus as a factor contributing to failure of antibiotic therapy.

Lactobacillus casei BL23 modulates the innate immune response in Staphylococcus aureus-stimulated bovine mammary epithelial cells

2018 ◽  
Vol 9 (6) ◽  
pp. 985-995 ◽  
Author(s):  
R.F.S. Souza ◽  
L. Rault ◽  
N. Seyffert ◽  
V. Azevedo ◽  
Y. Le Loir ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Response ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Lactobacillus Casei ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Innate Immune ◽  
Bovine Mammary Epithelial Cells ◽  
Aureus Infection

Probiotics have been adopted to treat and prevent various diseases in humans and animals. They were notably shown to be a promising alternative to prevent mastitis in dairy cattle. This inflammation of the mammary gland is generally of infectious origin and generates extensive economic losses worldwide. In a previous study, we found that Lactobacillus casei BL23 was able to inhibit the internalisation of Staphylococcus aureus, one of the major pathogens involved in mastitis, into bovine mammary epithelial cells (bMEC). In this study, we further explored the capacity of this strain to modulate the innate immune response of bovine mammary epithelial cells during S. aureus infection. L. casei BL23 was able to decrease the expression of several pro-inflammatory cytokines, including interleukins 6, 8, 1α and 1β and tumour necrosis factor alpha, in S. aureus-stimulated bMEC, 8 h post-infection. On the other hand, L. casei did not impair the induction of defensins, such as lingual antimicrobial peptide and defensin β1 in the presence of S. aureus, and even slightly increased the induction of tracheal antimicrobial peptide during S. aureus infection. Finally, this strain did not alter the expression of the pattern recognition receptor nucleotide-binding oligomerisation domain proteins (NOD2). This study demonstrates that L. casei BL23 displayed anti-inflammatory properties on S. aureus-stimulated bMEC. These results open the way to further characterisation of the BL23 probiotic potential in a bovine mammary gland context and to a better understanding of how all these beneficial properties combine in vivo to combat mastitis pathogens.

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