scholarly journals Three-Dimensional Analysis of Busulfan-Induced Spermatogenesis Disorder in Mice

2020 ◽  
Vol 8 ◽  
Author(s):  
Hiroki Nakata ◽  
Taito Nakano ◽  
Shoichi Iseki ◽  
Atsushi Mizokami
Keyword(s):  
3D Reconstruction ◽  
Dimensional Analysis ◽  
Intraperitoneal Injection ◽  
Three Dimensional ◽  
Stage I ◽  
Seminiferous Tubules ◽  
Factors Influencing ◽  
Branching Points ◽  
Normal Spermatogenesis ◽  
Single Testis

We examined if the distribution of impaired or normal spermatogenesis differs along the length of seminiferous tubules in disorders of spermatogenesis. For this purpose, three-dimensional (3D) reconstruction of seminiferous tubules was performed in mice with experimental spermatogenesis disorder induced by intraperitoneal injection of busulfan, and the areas of impaired and normal spermatogenesis were analyzed microscopically. The volume of the testis and length of seminiferous tubules decreased, and the proportion of tubule areas with impaired spermatogenesis increased depending on the dose of busulfan. With the highest dose of busulfan, although the proportion of impaired spermatogenesis was similar among individual seminiferous tubules, it was slightly but significantly higher in shorter tubules and in tubule areas near branching points. The tubule areas with impaired and normal spermatogenesis consisted of many segments of varying lengths. With increasing doses of busulfan, the markedly impaired segments increased in length without changing in number, whereas normal segments, although reduced in number and length, remained even with the highest dose of busulfan. Individual remaining normal segments consisted of several different stages, among which stage I and XII were found at higher frequencies, and stage VI at a lower frequency than expected in normal seminiferous tubules. We also examined if the distribution of impaired or normal spermatogenesis differs among different 3D positions in the testis without considering the course of seminiferous tubules. Although the proportions of impaired spermatogenesis with the minimum dose of busulfan and normal spermatogenesis with the highest dose of busulfan greatly varied by location within a single testis, there were no 3D positions with these specific proportions common to different testes, suggesting that the factors influencing the severity of busulfan-induced spermatogenesis disorder are not fixed in location among individual mice.

scholarly journals Three-dimensional analysis of seminiferous tubules and spermatogenic waves in mice

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 569-579 ◽  
Author(s):  
Hiroki Nakata ◽  
Takahiro Sonomura ◽  
Shoichi Iseki
Keyword(s):  
High Performance ◽  
Average Length ◽  
Periodic Acid ◽  
3D Structure ◽  
Three Dimensional ◽  
Rete Testis ◽  
Seminiferous Tubules ◽  
Periodic Acid Schiff ◽  
Adult Mice ◽  
Branching Points

The aim of the present study was to reconstruct seminiferous tubules and analyze spermatogenic waves in seminiferous epithelia in developing and adult mice using serial paraffin sections and high-performance three-dimensional (3D) reconstruction software. By labeling the basement membrane of seminiferous tubules with fluorescent immunohistochemistry or periodic acid-Schiff-hematoxylin staining, all seminiferous tubules were reconstructed in 9 testes from 9 different mice, 3 each at 0, 21 and 90 days (adult) postpartum. The 3D structure of seminiferous tubules, including the number and length of tubules as well as the number of connections with the rete testis, branching points and blind ends, was assessed accurately. Although tubules showed marked variations among individual mice, their overall structure was regular and retained from newborn to adult mice. Some seminiferous tubules contained inner portions running distant from the testis surface. In a representative testis at 21 days, the sites at which spermatids initially occurred were examined by labeling acrosomes and were found to be preferentially distributed in the upper and medial portions of the testis close to the rete testis. In a representative adult testis, 76 complete waves with an average length of 16.9 mm were found and their directions were analyzed. The methods used in the present study will be useful for investigating the structure and function of seminiferous tubules in mice and humans under normal and pathological conditions, such as infertility.

scholarly journals Plastic Deformation and Crystalline Texture in Equibiaxial Expansion

1979 ◽  
Vol 3 (3) ◽  
pp. 149-168 ◽  
Author(s):  
C. Gaspard ◽  
P. Messien ◽  
J. Mignon ◽  
T. Greday
Keyword(s):  
Plastic Deformation ◽  
Dimensional Analysis ◽  
Three Dimensional ◽  
Shear Stresses ◽  
Deformation Model ◽  
Theoretical Treatment ◽  
Slip Systems ◽  
Factors Influencing ◽  
Experimental Behavior ◽  
Shear Planes

The classical three-dimensional analysis (O.D.F.) has been extended in such a manner as to obtain the representation of the texture, and, more precisely, the distribution of the slip systems <111> {hkℓ} in the planes where shear stresses induced in equibiaxial expansion are maximum.Quantification of the slip systems configuration allows the development of a deformation model which takes into account the factors influencing the degree of stretchability of the steels. From this model it is possible to define true hardening rates and equibiaxiality coefficients characterizing the ability of the steel to distribute the deformation in the macroscopic shear planes.In association with the resistance to thinning defined by the configuration of the slip systems, the theoretical treatment makes it possible to grade different steels according to their experimental behavior.

Three-dimensional analysis of connexin43 gap junction in the ex vivo rat seminiferous tubules: Short-term effects of hormonal effectors

2009 ◽  
Vol 72 (11) ◽  
pp. 845-855 ◽  
Author(s):  
Jerome Gilleron ◽  
Diane Carette ◽  
Francoise Carpentier ◽  
Dominique Segretain ◽  
Georges Pointis
Keyword(s):  
Gap Junction ◽  
Dimensional Analysis ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Seminiferous Tubules ◽  
Short Term ◽  
Short Term Effects

The 3D Imaging and Metrology of CMSX-4 Superalloy Microstructure Using FIB-SEM Tomography Method

2013 ◽  
Vol 197 ◽  
pp. 89-94 ◽  
Author(s):  
Adam Kruk ◽  
Beata Dubiel ◽  
Aleksandra Czyrska-Filemonowicz
Keyword(s):  
3D Reconstruction ◽  
Single Crystal ◽  
Dimensional Analysis ◽  
Quantitative Data ◽  
3D Imaging ◽  
Volume Fraction ◽  
Microstructural Analysis ◽  
Three Dimensional ◽  
Operating Conditions ◽  
Tomography Method

STEM-EDX and FIB-SEM tomography studies have been carried out to visualize three-dimensional morphology of the γ’ precipitates in different zones of ex-service turbine blade made of CMSX-4 single crystal superalloy. The results allowed the three dimensional analysis of the changes in microstructure of blade as resulting of operating conditions. Tomographic reconstructions provided quantitative data about γ and γ’ phase shape, size and volume fraction. It was shown that FIB-SEM tomography technique is suitable for 3D reconstruction of the objects of 100 nm in size or even smaller and thus enables the accurate quantitative microstructural analysis of this superalloy.

scholarly journals Three-dimensional morphological analysis of spermatogenesis in aged mouse testes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Taito Nakano ◽  
Hiroki Nakata ◽  
Suguru Kadomoto ◽  
Hiroaki Iwamoto ◽  
Hiroshi Yaegashi ◽  
...  
Keyword(s):  
Morphological Analysis ◽  
Continuous Process ◽  
Three Dimensional ◽  
Basic Structure ◽  
Rete Testis ◽  
Three Dimensions ◽  
Seminiferous Tubules ◽  
Aged Mouse ◽  
Age Related ◽  
Branching Points

AbstractSpermatogenesis, which is a continuous process from undifferentiated spermatogonia to spermatozoa in the seminiferous tubules, declines with age. To investigate changes in spermatogenesis with aging, we reconstructed the seminiferous tubules of 12 mice aged 12 to 30 months from serial sections and examined age-related and region-specific alterations in the seminiferous epithelium and spermatogenic waves in three dimensions. The basic structure of the seminiferous tubules, including the numbers of tubules, terminating points, branching points, and total tubule length, did not change with age. Age-related alterations in spermatogenesis, primarily assessed by the formation of vacuoles in Sertoli cells, were detected in the seminiferous tubules at 12 months. The proportion of altered tubule segments with impaired spermatogenesis further increased by 24 months, but remained unchanged thereafter. Altered tubule segments were preferentially distributed in tubule areas close to the rete testis and those in the center of the testis. Spermatogenic waves became shorter in length with age. These results provide a basis for examining the decline of spermatogenesis not only with aging, but also in male infertility.

In situ investigation of the primary recrystallization of alpha titanium in HVEM

1978 ◽  
Vol 36 (1) ◽  
pp. 634-635
Author(s):  
S. Naka ◽  
R. Penelle ◽  
R. Valle
Keyword(s):  
Dimensional Analysis ◽  
Texture Evolution ◽  
Cold Work ◽  
Three Dimensional ◽  
Primary Recrystallization ◽  
Thin Foils ◽  
In Situ Investigation ◽  
Grain Growth Model ◽  
Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

Detection, Averaging, and 3D Reconstruction of Biological Specimens on Hypercubes (Transputer-based) Computers

1990 ◽  
Vol 48 (1) ◽  
pp. 454-455
Author(s):  
Jose-Maria Carazo ◽  
I. Benavides ◽  
S. Marco ◽  
J.L. Carrascosa ◽  
E.L. Zapata
Keyword(s):  
3D Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Software Tools ◽  
Mapping Method ◽  
Computer Architectures ◽  
Parallel Computer ◽  
Reconstruction Process ◽  
Computing Power ◽  
Order Of Magnitude

Obtaining the three-dimensional (3D) structure of negatively stained biological specimens at a resolution of, typically, 2 - 4 nm is becoming a relatively common practice in an increasing number of laboratories. A combination of new conceptual approaches, new software tools, and faster computers have made this situation possible. However, all these 3D reconstruction processes are quite computer intensive, and the middle term future is full of suggestions entailing an even greater need of computing power. Up to now all published 3D reconstructions in this field have been performed on conventional (sequential) computers, but it is a fact that new parallel computer architectures represent the potential of order-of-magnitude increases in computing power and should, therefore, be considered for their possible application in the most computing intensive tasks.We have studied both shared-memory-based computer architectures, like the BBN Butterfly, and local-memory-based architectures, mainly hypercubes implemented on transputers, where we have used the algorithmic mapping method proposed by Zapata el at. In this work we have developed the basic software tools needed to obtain a 3D reconstruction from non-crystalline specimens (“single particles”) using the so-called Random Conical Tilt Series Method. We start from a pair of images presenting the same field, first tilted (by ≃55°) and then untilted. It is then assumed that we can supply the system with the image of the particle we are looking for (ideally, a 2D average from a previous study) and with a matrix describing the geometrical relationships between the tilted and untilted fields (this step is now accomplished by interactively marking a few pairs of corresponding features in the two fields). From here on the 3D reconstruction process may be run automatically.

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