scholarly journals The Diversity and Similarity of Transmembrane Trimerization of TNF Receptors

2020 ◽  
Vol 8 ◽  
Author(s):  
Linlin Zhao ◽  
Qingshan Fu ◽  
Liqiang Pan ◽  
Alessandro Piai ◽  
James J. Chou
Keyword(s):  
Tnf Receptors

scholarly journals Tumor necrosis factor (TNF)-induced protein phosphorylation in a human rhabdomyosarcoma cell line is mediated by 60-kD TNF receptors (TR60)

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2211-2220 ◽  
Author(s):  
A Mire-Sluis ◽  
A Meager
Keyword(s):  
Tumor Necrosis Factor ◽  
Protein Phosphorylation ◽  
Cell Line ◽  
Tumor Necrosis ◽  
Tnf Alpha ◽  
Tnf Receptors ◽  
Protein Kinase C Inhibitors ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Necrosis Factor

Abstract In the present study, we used a cloned derivative, KYM-1D4, of the human rhabdomyosarcoma cell line, KYM-1, known to express high numbers of the two tumor necrosis factor (TNF) receptors, TR60 and TR80, and to be highly sensitive to TNF alpha-mediated cytotoxicity/antiproliferation, to investigate the role of TR60 and TR80 in protein phosphorylation. Using permeabilized KYM-1D4 cells, it was found that TNF alpha strongly induced phosphorylation of proteins of molecular weight 80, 65, 58, 42, and 30 kD. Addition of a monoclonal antibody (MoAb) against TR60 was shown to induce cytotoxicity/antiproliferation in KYM-1D4 cells and the same pattern of protein phosphorylation as TNF alpha, whereas addition of an MoAb against TR80 was both noncytotoxic and ineffective in inducing protein phosphorylation. In contrast, in a highly TNF alpha-resistant KYM-1- derived cell line, 37B8R, no protein phosphorylation was induced with either TNF alpha or the agonistic anti-TR60 MoAb. However, when 37B8R was allowed to revert to partial TNF sensitivity by culture in the absence of TNF alpha, the resultant cell line, 37B8S, was found to regain inducibility of protein phosphorylation by TNF alpha. These results indicate that expression of functional TR60 in KYM-1-related cell lines is principally involved in TNF-mediated cytotoxicity/antiproliferation and is necessary for the induction of protein phosphorylation. Nevertheless, the latter, although apparently strongly associated with cytotoxicity, was probably involved in protective mechanisms because protein kinase C inhibitors that inhibited TNF alpha and anti-TR60-induced phosphorylation increased the cytotoxic/antiproliferative response to these mediators.

scholarly journals POS0500 NUMBER AND CO-EXPRESSION OF TNF RECEPTORS TYPE 1 AND 2 ON IMMUNOCOMPETENT CELLS ARE ASSOCIATED WITH STABILITY OF REMISSION IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 482.2-483
Author(s):  
A. Alshevskaya ◽  
J. Lopatnikova ◽  
J. Zhukova ◽  
O. Chumasova ◽  
N. Shkaruba ◽  
...  
Keyword(s):  
Disease Activity ◽  
T Regulatory Cells ◽  
Lower Percentage ◽  
Tnf Receptors ◽  
Double Positive ◽  
Low Disease Activity ◽  
Quantitative Expression ◽  
Healthy Donors

Background:The balance of TNFα receptors expression on cells which are actively involved in immunopathological processes affects both the density of distribution of receptors on cells and co-expression in subsets. Previously it was shown that basic effective RA therapy with methotrexate and glucocorticoids leads to equalization of the expression profile either in the percentage of cells or in the number of receptors, approaching those of healthy donors, but not simultaneously. However, questions about the relationship between the effectiveness of biological therapy and receptors co-expression remain unknown.Objectives:To assess the differences in co-expression and quantitative expression of TNF receptors type 1 and 2 in subsets of cells associated with the severity of the disease, depending on the response to rituximab therapy.Methods:Subanalysis of patients with high disease activity level successfully treated with rituximab (alone or in combination treatment scheme) during hospitalization was performed (n = 14). The first group included 6 patients who retained low disease activity during 1 month follow-up (RA, stabilization). The second group consisted of 8 patients who had exacerbation during follow-up period. As a control group, we used data from 43 comparable healthy donors. Subsets of T regulatory cells and monocytes were studied. A comparison was made among the indicators of receptors number and proportion of cells expressing the corresponding receptor.Results:For T regulatory cells, the key differences for patients who did not retain low disease activity were significantly higher number of TNF type 1 and type 2 receptors on double-positive cells with a lower percentage of these cells compared to stable patients. At the same time, higher differences between proportions of double-positive cells in comparison with control values of healthy donors were associated with higher probability of maintaining in remission.For monocytes, the key differences in stable patients were the very high quantitative expression of type 1 receptors on double-positive cells, with a lower percentage of these cells compared to patients with exacerbation. At the same time, lower differences between proportions of double-positive cells in comparison with control values of healthy donors were associated with higher probability of maintaining in remission.Conclusion:Obtained data confirm the previously proposed hypothesis about the essential role of balance in quantitative expression of TNF receptors type 1 and 2 on double-positive cells to determine the intensity and type of cell response to the mediator and its association with the level of disease activity and response to therapy.Acknowledgements:This study is supported by grant of the President of the Russian Federation for state support of young Russian PhD scientists №МК-2433.2020.4Disclosure of Interests:None declared

TNF signaling via TNF receptors does not mediate the effects of short-term exercise on cognition, anxiety and depressive-like behaviors in middle-aged mice

2021 ◽  
pp. 113269
Author(s):  
Gaurav Singhal ◽  
Magdalene C. Jawahar ◽  
Julie Morgan ◽  
Frances Corrigan ◽  
Emily J. Jaehne ◽  
...  
Keyword(s):  
Tnf Receptors ◽  
Middle Aged ◽  
Short Term ◽  
Aged Mice

scholarly journals Targeted Disruption of the Mouse Caspase 8 Gene Ablates Cell Death Induction by the TNF Receptors, Fas/Apo1, and DR3 and Is Lethal Prenatally

Immunity ◽  
1998 ◽  
Vol 9 (2) ◽  
pp. 267-276 ◽  
Author(s):  
Eugene E Varfolomeev ◽  
Marcus Schuchmann ◽  
Victor Luria ◽  
Nuchanard Chiannilkulchai ◽  
Jacques S Beckmann ◽  
...  
Keyword(s):  
Cell Death ◽  
Caspase 8 ◽  
Tnf Receptors ◽  
Targeted Disruption

scholarly journals Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis

2009 ◽  
Vol 60 (1-3) ◽  
pp. 161-164 ◽  
Author(s):  
Hiroaki Ida ◽  
Toshiyuki Aramaki ◽  
Hideki Nakamura ◽  
Keita Fujikawa ◽  
Kazuhiko Arima ◽  
...  
Keyword(s):  
Flow Cytometric Analysis ◽  
Tnf Receptors ◽  
Expression Levels ◽  
Flow Cytometric

scholarly journals Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells

1998 ◽  
Vol 28 (11) ◽  
pp. 3577-3586 ◽  
Author(s):  
Rudolf Lucas ◽  
Irene Garcia ◽  
Yves R. A. Donati ◽  
Marusa Hribar ◽  
Stefano J. Mandriota ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Tnf Receptors ◽  
Microvascular Endothelial

scholarly journals Characterization of TNF receptor subtype expression and signaling on pulmonary endothelial cells in mice

2011 ◽  
Vol 300 (5) ◽  
pp. L781-L789 ◽  
Author(s):  
Szabolcs Bertok ◽  
Michael R. Wilson ◽  
Anthony D. Dorr ◽  
Justina O. Dokpesi ◽  
Kieran P. O'Dea ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Adhesion Molecules ◽  
Surface Expression ◽  
Tnf Receptor ◽  
Wild Type ◽  
Tnf Receptors ◽  
Pulmonary Endothelial Cells ◽  
Microvascular Inflammation

TNF plays a crucial role in the pathogenesis of acute lung injury. However, the expression profile of its two receptors, p55 and p75, on pulmonary endothelium and their influence on TNF signaling during lung microvascular inflammation remain uncertain. Using flow cytometry, we characterized the expression profile of TNF receptors on the surface of freshly harvested pulmonary endothelial cells (PECs) from mice and found expression of both receptors with dominance of p55. To investigate the impact of stimulating individual TNF receptors, we treated wild-type and TNF receptor knockout mice with intravenous TNF and determined surface expression of adhesion molecules (E-selectin, VCAM-1, ICAM-1) on PECs by flow cytometry. TNF-induced upregulation of all adhesion molecules was substantially attenuated by absence of p55, whereas lack of p75 had a similar but smaller effect that varied between adhesion molecules. Selective blockade of individual TNF receptors by specific antibodies in wild-type primary PEC culture confirmed that the in vivo findings were due to direct effects of TNF receptor inhibition on endothelium and not other cells (e.g., circulating leukocytes). Finally, we found that PEC surface expression of p55 dramatically decreased in the early stages of endotoxemia following intravenous LPS, while no change in p75 expression was detected. These data demonstrate a crucial in vivo role of p55 and an auxiliary role of p75 in TNF-mediated adhesion molecule upregulation on PECs. It is possible that the importance of the individual receptors varies at different stages of pulmonary microvascular inflammation following changes in their relative expression.

Differential regulation of TNF receptors by vagal nerve stimulation protects heart against acute ischemic injury

2010 ◽  
Vol 49 (2) ◽  
pp. 234-244 ◽  
Author(s):  
Rajesh G. Katare ◽  
Motonori Ando ◽  
Yoshihiko Kakinuma ◽  
Mikihiko Arikawa ◽  
Fumiyasu Yamasaki ◽  
...  
Keyword(s):  
Nerve Stimulation ◽  
Ischemic Injury ◽  
Differential Regulation ◽  
Vagal Nerve ◽  
Vagal Nerve Stimulation ◽  
Tnf Receptors
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