scholarly journals Mesenchymal Stromal Cell-Produced Components of Extracellular Matrix Potentiate Multipotent Stem Cell Response to Differentiation Stimuli

2020 ◽  
Vol 8 ◽  
Author(s):  
Ekaterina Novoseletskaya ◽  
Olga Grigorieva ◽  
Peter Nimiritsky ◽  
Nataliya Basalova ◽  
Roman Eremichev ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Response ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Multipotent Stem Cell

Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration

Cytotherapy ◽  
2015 ◽  
Vol 17 (7) ◽  
pp. 860-873 ◽  
Author(s):  
Mizuho Kittaka ◽  
Mikihito Kajiya ◽  
Hideki Shiba ◽  
Manabu Takewaki ◽  
Kei Takeshita ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell

Bioprinting 3D cell-laden hydrogel microarray for screening human periodontal ligament stem cell response to extracellular matrix

2015 ◽  
Vol 7 (4) ◽  
pp. 044105 ◽  
Author(s):  
Yufei Ma ◽  
Yuan Ji ◽  
Guoyou Huang ◽  
Kai Ling ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Response ◽  
Periodontal Ligament ◽  
Periodontal Ligament Stem Cell

Regulation of β1-Integrin by Mir-134 in Mesenchymal Stromal Cells – Implications for Mesenchymal Stromal Cell Adherence and Hematopoietic Stem Cell Interaction

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 3459-3459
Author(s):  
Friedrich Stölzel ◽  
David M. Poitz ◽  
Laleh S. Arabanian ◽  
Jens Friedrichs ◽  
Denitsa Docheva ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Luciferase Activity ◽  
Cell Types ◽  
Bone Marrow Niche ◽  
Β1 Integrin ◽  
Hematopoietic Stem ◽  
Transfected Cells

Abstract Abstract 3459 The different intra- and extracellular constituents of the hematopoietic stem cell (HSC) niche in the human bone marrow are tightly regulated and of momentous importance for various properties of HSCs. Some of these are regulated through β1-Integrins (CD29) which therefore dramatically influence HSC and mesenchymal stromal cell (MSC) interaction in the niche. Important regulators within these cells are microRNAs (miRNAs). These small, non-coding RNAs control the expression of around two-thirds of the human protein-coding genes. One of these miRNAs, miR-134, previously referred to be a “brain-specific” miRNA was shown to be highly expressed in MSCs in tissue-studies conducted by our group. Since the central nervous system was recently shown to be closely connected to the regulation of HSCs and MSCs, we asked whether miR-134 which has several conserved binding seed-match sequences within the 3'UTR of β1-Integrin, regulates MSC mediated properties in the bone marrow niche. Screening of human MSC cell lines (n=4) by western blotting revealed highest β1-Integrin expression in SCP-1 cells. Transfection of SCP-1 with either siRNA directed against β1-Integrin (siCD29) or pre-miRNA-134 (pre134) revealed a downregulation of β1-Integrin at the mRNA level only in siRNA transfected cells, p=0.01. In contrast, at the protein level, as measured by western blot and FACS analysis, p=0.002, β1-Integrin was downregulated by siCD29 as well as by pre134, indicating a miRNA-specific action of repression. Confirmatory, the 3'UTR of β1-Integrin, which contains several putative binding sites for miR-134, was cloned into a pMiRReporter vector and luciferase activity was measured after cotransfection with pre134. The luciferase activity was significantly reduced in pre134 transfected cells [1.80 ± 0.46 (preCo) vs. 0.99 ± 0.49 (pre134); p<0.001]. To evaluate whether pre134 mediated reduction of β1-Integrin can modulate the adhesion potential of SCP-1, atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) was performed. Indeed, transfection of SCP-1 with siCD29 or pre134 resulted in a significantly reduced adherence as compared to their respective controls, p<0.001 and p<0.01. Furthermore, using AFM-based SCFS we investigated the interaction between 32D-cells, which have a high surface expression of the natural interaction partner of β1-Integrin VCAM-1, and SCP-1 cells. Here again, we were able to show, that 32D show a significantly lower adhesion potential to siCD29 and pre134-transfected SCP-1, p<0.001 and p<0.001, respectively. In a translational approach MSCs from healthy bone marrow donors (n=30) and from MDS patients (n=17) were screened for miRNA-expression. This analysis revealed 50% higher miR-134 transcript levels in MSCs from MDS patients [0.0057 ± 0.0021 (healthy) vs. 0.0127 ± 0.0045 (MDS); p<0.001], suggesting a potential role of this miRNA in regulating its MSC adhesion. Regulation of adhesion of MSCs and to MSCs is important for various components of the bone marrow niche. Here, we demonstrate for the first time that β1-Integrin mediated adhesion of MSCs themselves and other cell types onto MSCs via β1-Integrin receptors can be inhibited via miR-134 overexpression. Furthermore, this newly characterized mechanism provides evidence for a potential anti-adhesive influence of miR-134. While this might not only influence adhesion, other mechanisms such as homing of HSCs as well as other cell types, might be affected by modification of miR-134 expression in the stromal niche. Disclosures: Platzbecker: Amgen: Consultancy; GlaxoSmithKline: Consultancy; Celgene: Consultancy; Novartis: Consultancy.

Human embryonic stem cell-derived mesenchymal stromal cell transplantation in a rat hind limb injury model

Cytotherapy ◽  
2009 ◽  
pp. 1-12
Author(s):  
Juha Laurila ◽  
Lilja Laatikainen ◽  
Maria Castellone ◽  
Parul Trivedi ◽  
Jari Heikkila ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Transplantation ◽  
Mesenchymal Stromal Cell ◽  
Hind Limb ◽  
Human Embryonic Stem Cell ◽  
Stromal Cell ◽  
Embryonic Stem ◽  
Limb Injury ◽  
Injury Model

Methods for the Assembly and Characterization of Polyelectrolyte Multilayers as Microenvironments to Modulate Human Mesenchymal Stromal Cell Response

2020 ◽  
Vol 6 (12) ◽  
pp. 6626-6651
Author(s):  
David A. Castilla-Casadiego ◽  
Hemanta Timsina ◽  
Mahsa Haseli ◽  
Luis Pinzon-Herrera ◽  
Yu-Hsuan Chiao ◽  
...  
Keyword(s):  
Cell Response ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Polyelectrolyte Multilayers

Hierarchically Demineralized Cortical Bone Combined With Stem Cell–Derived Extracellular Matrix for Regeneration of the Tendon-Bone Interface

2021 ◽  
pp. 036354652199451
Author(s):  
Shu-Kun He ◽  
Liang-Ju Ning ◽  
Xuan Yao ◽  
Ruo-Nan Hu ◽  
Jing Cui ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Rotator Cuff ◽  
Cortical Bone ◽  
Immunohistochemical Staining ◽  
Stromal Cell ◽  
Biomechanical Analysis ◽  
Immunofluorescence Staining ◽  
Bone Interface ◽  
Histological Staining

Background: Poor healing of the tendon-bone interface after rotator cuff repair is one of the main causes of surgical failure. Previous studies demonstrated that demineralized cortical bone (DCB) could improve healing of the enthesis. Purpose: To evaluate the outcomes of hierarchically demineralized cortical bone (hDCB) coated with stem cell–derived extracellular matrix (hDCB-ECM) in the repair of the rotator cuff in a rabbit model. Study Design: Controlled laboratory study. Methods: Tendon-derived stem cells (TDSCs) were isolated, cultured, and identified. Then, hDCB was prepared by the graded demineralization procedure. Finally, hDCB-ECM was fabricated via 2-week cell culture and decellularization, and the morphologic features and biochemical compositions of the hDCB-ECM were evaluated. A total of 24 rabbits (48 samples) were randomly divided into 4 groups: control, DCB, hDCB, and hDCB-ECM. All rabbits underwent bilateral detachment of the infraspinatus tendon, and the tendon-bone interface was repaired with or without scaffolds. After surgery, 8 rabbits were assessed by immunofluorescence staining at 2 weeks, and the others were assessed by micro–computed tomography (CT) examination, immunohistochemical staining, histological staining, and biomechanical testing at 12 weeks. Results: TDSCs were identified to have universal stem cell characteristics including cell markers, clonogenicity, and multilineage differentiation. The hDCB-ECM contained 3 components (bone, partial DCB, and DCB coated with ECM) with a gradient of calcium and phosphorus elements, and the ECM had stromal cell-derived factor 1, biglycan, and fibromodulin. Macroscopic observations demonstrated the absence of infection and rupture around the enthesis. The results of immunofluorescence staining showed that hDCB-ECM promoted stromal cell recruitment. Results of micro-CT analysis, immunohistochemical staining, and histological staining showed that hDCB-ECM enhanced bone and fibrocartilage formation at the tendon-bone interface. Biomechanical analysis showed that the hDCB-ECM group had higher ultimate tensile stress and Young modulus than the DCB group. Conclusion: The administration of hDCB-ECM promoted healing of the tendon-bone interface. Clinical Relevance: hDCB-ECM could provide useful information for the design of scaffolds to repair the tendon-bone interface, and further studies are needed to determine its effectiveness.

Synthetic extracellular matrix mimic hydrogel improves efficacy of mesenchymal stromal cell therapy for ischemic cardiomyopathy

2018 ◽  
Vol 70 ◽  
pp. 71-83 ◽  
Author(s):  
Maria Chiara Ciuffreda ◽  
Giuseppe Malpasso ◽  
Cindy Chokoza ◽  
Deon Bezuidenhout ◽  
Kyle P. Goetsch ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Therapy ◽  
Mesenchymal Stromal Cell ◽  
Ischemic Cardiomyopathy ◽  
Stromal Cell

Heparin/Collagen Coatings Improve Human Mesenchymal Stromal Cell Response to Interferon Gamma

2019 ◽  
Vol 5 (6) ◽  
pp. 2793-2803 ◽  
Author(s):  
David A. Castilla-Casadiego ◽  
José R. García ◽  
Andrés J. García ◽  
Jorge Almodovar
Keyword(s):  
Interferon Gamma ◽  
Cell Response ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell
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