scholarly journals Caspase-3 Activation Correlates With the Initial Mitochondrial Membrane Depolarization in Neonatal Cerebellar Granule Neurons

2020 ◽  
Vol 8 ◽  
Author(s):  
Edaena Benítez-Rangel ◽  
Mauricio Olguín-Albuerne ◽  
María Cristina López-Méndez ◽  
Guadalupe Domínguez-Macouzet ◽  
Agustín Guerrero-Hernández ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Membrane Depolarization ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Mitochondrial Membrane Depolarization

scholarly journals Procaspase-activating compound 1 induces a caspase-3-dependent cell death in cerebellar granule neurons

2010 ◽  
Vol 247 (3) ◽  
pp. 238-242 ◽  
Author(s):  
Gulzeb Aziz ◽  
Øyvind W. Akselsen ◽  
Trond V. Hansen ◽  
Ragnhild E. Paulsen
Keyword(s):  
Cell Death ◽  
Caspase 3 ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Dependent Cell

Inactivation of aconitase during the apoptosis of mouse cerebellar granule neurons induced by a deprivation of membrane depolarization

2003 ◽  
Vol 71 (4) ◽  
pp. 504-515 ◽  
Author(s):  
Akiko Tabuchi ◽  
Kouhei Funaji ◽  
Jun Nakatsubo ◽  
Mamoru Fukuchi ◽  
Tomofusa Tsuchiya ◽  
...  
Keyword(s):  
Membrane Depolarization ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule

Platelet Senescence and Phosphatidylserine Exposure.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2117-2117
Author(s):  
Swapan Kumar Dasgupta ◽  
Hanan Abdel-Monem ◽  
Anthony Prakasam ◽  
Perumal Thiagarajan
Keyword(s):  
Cytochrome C ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Conflicts Of Interest ◽  
Membrane Depolarization ◽  
Procoagulant Activity ◽  
Cyclophilin D ◽  
Cytochrome C Release ◽  
Phosphatidylserine Exposure ◽  
Mitochondrial Membrane Depolarization

Abstract Abstract 2117 Poster Board II-94 The transbilayer movement of phosphatidylserine from the inner to the outer leaflet of membrane bilayer occurs during platelet activation and is essential for platelet procoagulant activity. Expression of phosphatidylserine also occurs in platelets stored more than 5 days in the blood bank. We have examined the role of mitochondrial pathways in activation-induced platelet procoagulant activity and during in vitro senescence. Collagen and thrombin-induced exposure of phosphatidylserine is associated with a decrease in mitochondrial membrane potential (MMP). Cyclosporin A, a known inhibitor of cyclophilin D, inhibited phosphatidylserine exposure and the loss of MMP. Consistent with this, platelets from CypD deficient mice had decreased loss of MMP and impaired phosphatidylserine exposure when treated with a combination of thrombin and collagen. However, there is no release of cytochrome c into the cytosol. Also there is no activation of caspase-3 or Rho associated kinase I (ROCK1). In sharp contrast to this, in platelets stored for more than 5 days, phosphatidylserine exposure is found to be associated with caspase-3 and ROCK1 activation. To delineate the mechanism we used ABT737, a BH3 mimetic that induces mitochondrial pathway of apoptosis via Bcl-2 family of proteins. ABT737-induced phosphatidylserine exposure was also associated with cytochrome c release, caspase-3 and ROCKI activation and it is unaffected in platelets isolated from CypD knock out mice or in platelets treated with cyclosporine A prior to activation. Caspase-3 inhibitor (z-DVED-fmk and ROCK1 inhibitor Y-27632 reduced ABT-737 induced phosphatidylserine expression. Our results also show that activation-induced phosphatidylserine exposure is dependent on mitochondrial membrane depolarization and CypD but is independent of cytochrome c release or caspase-3 or ROCK1 activation. In contrast, the phosphatidylserine exposure in stored platelets occurs via mitochondrial cytochrome c release, caspase-3 activation and ROCKI activation and does not depend on mitochondrial membrane depolarization or CypD. Disclosures: No relevant conflicts of interest to declare.

Involvement of a Caspase-3-Like Cysteine Protease in 1-Methyl-4-Phenylpyridinium-Mediated Apoptosis of Cultured Cerebellar Granule Neurons

1997 ◽  
Vol 69 (4) ◽  
pp. 1382-1388 ◽  
Author(s):  
Yangshen Du ◽  
Richard C. Dodel ◽  
Kelly R. Bales ◽  
Ronald Jemmerson ◽  
Elizabeth Hamilton-Byrd ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Caspase 3 ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule
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