scholarly journals Characterization of Human Colon Organoids From Inflammatory Bowel Disease Patients

2020 ◽  
Vol 8 ◽  
Author(s):  
Emilie d’Aldebert ◽  
Muriel Quaranta ◽  
Morgane Sébert ◽  
Delphine Bonnet ◽  
Sylvain Kirzin ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Human Colon ◽  
Inflammatory Bowel

scholarly journals 4483 Activity and Abundance of Mucus-degrading Microbes in Inflammatory Bowel Disease

2020 ◽  
Vol 4 (s1) ◽  
pp. 92-92
Author(s):  
Matthew Ostrowski ◽  
Eric C. Martens
Keyword(s):  
Inflammatory Bowel Disease ◽  
New Species ◽  
Bowel Disease ◽  
Human Colon ◽  
Healthy Controls ◽  
Solid Culture ◽  
Rrna Sequencing ◽  
Study Population ◽  
Inflammatory Bowel

OBJECTIVES/GOALS: This study seeks to culture and characterize mucus-degrading microbes from the microbiome of inflammatory bowel disease (IBD) patients. METHODS/STUDY POPULATION: Stool will be collected from IBD patients and healthy first-degree relatives, then enriched for mucin-degrading microbes through growth on porcine rectal mucin. Dilution plating in both liquid and solid culture formats will be employed to isolate strains capable of growth on mucin. Cultures that are positive for mucin degradation will be identified with 16S rRNA sequencing; unique isolates will be genome sequenced and transcriptionally profiled on simple monosaccharides and mucin in order to identify putative mucin-degrading genes. The abundance of novel enzymes, pathways, and microbes will be compared in healthy and IBD patient populations using existing datasets in the literature. RESULTS/ANTICIPATED RESULTS: We expect to isolate previously uncultured mucin-degrading microbes, which will likely include new strains and possibly new species of bacteria. Through the transcriptomic characterization of mucin-degrading pathways, we will expand the lexicon of known mucin-degrading enzymes and pathways used by bacteria in the human colon. We expect mucin-degrading microbes to be more abundant and active in IBD patients compared to healthy controls. DISCUSSION/SIGNIFICANCE OF IMPACT: There is no cure for IBD and treatment relies heavily on suppressing a patient’s immune system. This research seeks to understand the contribution of the gut microbiota in the pathogenesis of IBD, which may lead to future therapeutic targets.

scholarly journals Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Mette Boyd ◽  
Malte Thodberg ◽  
Morana Vitezic ◽  
Jette Bornholdt ◽  
Kristoffer Vitting-Seerup ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Human Colon ◽  
Inflammatory Bowel

Characterization of Helicobacter-induced inflammatory bowel disease in IL-10 -/- and T cell deficient mice

2001 ◽  
Vol 120 (5) ◽  
pp. A523-A523
Author(s):  
A BURICH ◽  
R HERSHBERG ◽  
K WAGGIE ◽  
W ZENG ◽  
J VINEY ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
T Cell ◽  
Bowel Disease ◽  
Inflammatory Bowel ◽  
Deficient Mice

scholarly journals Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2−/−/Il10−/− Mouse Model

2020 ◽  
Vol 22 (1) ◽  
pp. 364
Author(s):  
Qiyuan Han ◽  
Thomas J. Y. Kono ◽  
Charles G. Knutson ◽  
Nicola M. Parry ◽  
Christopher L. Seiler ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Gastrointestinal Disease ◽  
Tumor Development ◽  
Proximal Colon ◽  
Helicobacter Hepaticus ◽  
Reduced Representation ◽  
Reduced Representation Bisulfite Sequencing ◽  
Inflammatory Bowel

Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In the present work, DNA methylome, hydroxymethylome, and transcriptome analyses were conducted in proximal colon tissues harvested from the Helicobacter hepaticus (H. hepaticus)-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) analyses identified 1606 differentially methylated regions (DMR) and 3011 differentially hydroxymethylated regions (DhMR). These DMR/DhMR overlapped with genes that are associated with gastrointestinal disease, inflammatory disease, and cancer. RNA-seq revealed pronounced expression changes of a number of genes associated with inflammation and cancer. Several genes including Duox2, Tgm2, Cdhr5, and Hk2 exhibited changes in both DNA methylation/hydroxymethylation and gene expression levels. Overall, our results suggest that chronic inflammation triggers changes in methylation and hydroxymethylation patterns in the genome, altering the expression of key tumorigenesis genes and potentially contributing to the initiation of colorectal cancer.

Characterization of circulating interleukin-1 receptor antagonist expression in children with inflammatory bowel disease

1994 ◽  
Vol 39 (9) ◽  
pp. 1893-1899 ◽  
Author(s):  
Jeffrey S. Hyams ◽  
John E. Fitzgerald ◽  
Nancy Wyzga ◽  
William R. Treem ◽  
Christopher J. Justinich ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Receptor Antagonist ◽  
Bowel Disease ◽  
Interleukin 1 ◽  
Interleukin 1 Receptor Antagonist ◽  
Inflammatory Bowel

Characterization of Anti-Lymphocyte Antibodies in Inflammatory Bowel Disease

1976 ◽  
Vol 5 (6-7) ◽  
pp. 837-844 ◽  
Author(s):  
C. A. HENDERSON ◽  
L. GREENLEE ◽  
R. C. WILLIAMS ◽  
R.G. STRICKLAND
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Inflammatory Bowel

T1257 Characterization of Changes of Serum Anti-Glycan Antibodies in Individual Patients Over Time in Inflammatory Bowel Disease (IBD)

2010 ◽  
Vol 138 (5) ◽  
pp. S-522
Author(s):  
Florian Rieder ◽  
Stephan Schleder ◽  
Alexandra Wolf ◽  
Anja Schirbel ◽  
Andre Franke ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Inflammatory Bowel ◽  
Over Time
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