scholarly journals Endothelial Progenitor Cells Produced From Human Pluripotent Stem Cells by a Synergistic Combination of Cytokines, Small Compounds, and Serum-Free Medium

2020 ◽  
Vol 8 ◽  
Author(s):  
Simon Farkas ◽  
Pavel Simara ◽  
Daniela Rehakova ◽  
Lenka Veverkova ◽  
Irena Koutna
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Endothelial Progenitor ◽  
Serum Free ◽  
Synergistic Combination

scholarly journals Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium

2010 ◽  
Vol 46 (7) ◽  
pp. 573-576 ◽  
Author(s):  
Miho Kusuda Furue ◽  
Daiki Tateyama ◽  
Masaki Kinehara ◽  
Jie Na ◽  
Tetsuji Okamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells

2015 ◽  
Vol 15 (1) ◽  
pp. 122-129 ◽  
Author(s):  
Xiaoping Bao ◽  
Xiaojun Lian ◽  
Kaitlin K. Dunn ◽  
Mengxuan Shi ◽  
Tianxiao Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Endothelial Progenitor

scholarly journals The differentiation of pluripotent stem cells towards transplantable endothelial progenitor cells

2021 ◽  
Author(s):  
Kezhou Qin ◽  
Jun Yang
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Pulmonary Vasculature ◽  
Molecular Characteristics ◽  
Endothelial Progenitor ◽  
Highly Efficient ◽  
Differentiation Protocol ◽  
Healthy Donors

AbstractEndothelial progenitor cells (EPCs) and endothelial cells (ECs) have been applied in the clinic to treat pulmonary arterial hypertension (PAH), a disease characterized by disordered pulmonary vasculature. However, the lack of sufficient transplantable cells before the deterioration of disease condition is a current limitation to apply cell therapy in patients. It is necessary to differentiate pluripotent stem cells (PSCs) into EPCs and identify their characteristics. Comparing previously reported methods of human PSCs-derived ECs, we optimized a highly efficient differentiation protocol to obtain cells that match the phenotype of isolated EPCs from healthy donors. The protocol is compatible with chemically defined medium (CDM), it could produce a large number of clinically applicable cells with low cost. Moreover, we also found PSCs-derived EPCs express CD133, have some characteristics of mesenchymal stem cells and are capable of homing to repair blood vessels in zebrafish xenograft assays. In addition, we further revealed that IPAH PSCs-derived EPCs have higher expression of proliferation-related genes and lower expression of immune-related genes than normal EPCs and PSCs-derived EPCs through microarray analysis. In conclusion, we optimized a highly efficient differentiation protocol to obtain PSCs-derived EPCs with the phenotypic and molecular characteristics of EPCs from healthy donors which distinguished them from EPCs from PAH.

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