scholarly journals Human Obesity Induces Dysfunction and Early Senescence in Adipose Tissue-Derived Mesenchymal Stromal/Stem Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Sabena M. Conley ◽  
LaTonya J. Hickson ◽  
Todd A. Kellogg ◽  
Travis McKenzie ◽  
Julie K. Heimbach ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Human Obesity ◽  
Stromal Stem Cells

scholarly journals Adipose-Derived Mesenchymal Stromal/Stem Cells: Tissue Localization, Characterization, and Heterogeneity

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Patrick C. Baer ◽  
Helmut Geiger
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Cultured Cells ◽  
Current Knowledge ◽  
High Plasticity ◽  
Culture Methods ◽  
Tissue Localization ◽  
Stromal Stem Cells

Adipose tissue as a stem cell source is ubiquitously available and has several advantages compared to other sources. It is easily accessible in large quantities with minimal invasive harvesting procedure, and isolation of adipose-derived mesenchymal stromal/stem cells (ASCs) yields a high amount of stem cells, which is essential for stem-cell-based therapies and tissue engineering. Several studies have provided evidence that ASCs in situ reside in a perivascular niche, whereas the exact localization of ASCs in native adipose tissue is still under debate. ASCs are isolated by their capacity to adhere to plastic. Nevertheless, recent isolation and culture techniques lack standardization. Cultured cells are characterized by their expression of characteristic markers and their capacity to differentiate into cells from meso-, ecto-, and entodermal lineages. ASCs possess a high plasticity and differentiate into various cell types, including adipocytes, osteoblasts, chondrocytes, myocytes, hepatocytes, neural cells, and endothelial and epithelial cells. Nevertheless, recent studies suggest that ASCs are a heterogeneous mixture of cells containing subpopulations of stem and more committed progenitor cells. This paper summarizes and discusses the current knowledge of the tissue localization of ASCs in situ, their characterization and heterogeneityin vitro, and the lack of standardization in isolation and culture methods.

Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy

2016 ◽  
pp. 137-146 ◽  
Author(s):  
Fabiana Zanata ◽  
Shahensha Shaik ◽  
Ram V. Devireddy ◽  
Xiying Wu ◽  
Lydia Masako Ferreira ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stromal Stem Cells

scholarly journals Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1346 ◽  
Author(s):  
Baer ◽  
Koch ◽  
Hickmann ◽  
Schubert ◽  
Cinatl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Epithelial Cells ◽  
Lipoteichoic Acid ◽  
Differentiation Potential ◽  
Perivascular Niche ◽  
Perirenal Adipose Tissue ◽  
Multipotent Cells ◽  
Stromal Stem Cells

Mesenchymal stromal/stem cells (MSCs) are immature multipotent cells, which represent a rare population in the perivascular niche within nearly all tissues. The most abundant source to isolate MSCs is adipose tissue. Currently, perirenal adipose tissue is rarely described as the source of MSCs. MSCs were isolated from perirenal adipose tissue (prASCs) from patients undergoing tumor nephrectomies, cultured and characterized by flow cytometry and their differentiation potential into adipocytes, chondrocytes, osteoblasts and epithelial cells. Furthermore, prASCs were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA) or a mixture of cytokines (cytomix). In addition, prASC susceptibility to human cytomegalovirus (HCMV) was investigated. The expression of inflammatory readouts was estimated by qPCR and immunoassay. HCMV infection was analyzed by qPCR and immunostaining. Characterization of cultured prASCs shows the cells meet the criteria of MSCs and prASCs can undergo trilineage differentiation. Cultured prASCs can be induced to differentiate into epithelial cells, shown by cytokeratin 18 expression. Stimulation of prASCs with LPS or cytomix suggests the cells are capable of initiating an inflammation-like response upon stimulation with LPS or cytokines, whereas, LTA did not induce a significant effect on the readouts (ICAM-1, IL-6, TNFα, MCP-1 mRNA and IL-6 protein). HCMV broadly infects prASCs, showing a viral load dependent cytopathological effect (CPE). Our current study summarizes the isolation and culture of prASCs, clearly characterizes the cells, and demonstrates their immunomodulatory potential and high permissiveness for HCMV.

scholarly journals Adipose-Derived Stromal/Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1997
Author(s):  
Patrick C. Baer
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Stromal Stem Cells

Adipose tissue is a rich, ubiquitous, and easily accessible source for multipotent mesenchymal stromal/stem cells (MSCs), so-called adipose-derived stromal/stem cells (ASCs) [...]

scholarly journals Comparison of infrapatellar and subcutaneous adipose tissue stromal vascular fraction and stromal/stem cells in osteoarthritic subjects

2012 ◽  
Vol 8 (10) ◽  
pp. 757-762 ◽  
Author(s):  
Pedro Pires de Carvalho ◽  
Katie M. Hamel ◽  
Robert Duarte ◽  
Andrew G. S. King ◽  
Masudul Haque ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Subcutaneous Adipose ◽  
Stromal Stem Cells

scholarly journals Stromal stem cells from adipose tissue and bone marrow of age-matched female donors display distinct immunophenotypic profiles

2010 ◽  
Vol 226 (3) ◽  
pp. 843-851 ◽  
Author(s):  
G. Pachón-Peña ◽  
G. Yu ◽  
A. Tucker ◽  
X. Wu ◽  
J. Vendrell ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Stromal Stem Cells

Involvement of P2X7 Receptors in the Osteogenic Differentiation of Mesenchymal Stromal/Stem Cells Derived from Human Subcutaneous Adipose Tissue

2019 ◽  
Vol 15 (4) ◽  
pp. 574-589 ◽  
Author(s):  
Marzia Carluccio ◽  
Mariachiara Zuccarini ◽  
Sihana Ziberi ◽  
Patricia Giuliani ◽  
Caterina Morabito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Subcutaneous Adipose Tissue ◽  
P2x7 Receptors ◽  
Subcutaneous Adipose ◽  
Stromal Stem Cells

scholarly journals The differentiation potential of adipose tissue-derived mesenchymal stem cells into cell lineage related to male germ cells

2018 ◽  
Vol 70 (1) ◽  
pp. 160-168 ◽  
Author(s):  
P. Bräunig ◽  
W.G. Glanzner ◽  
V.B. Rissi ◽  
P.B.D. Gonçalves
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Lineage ◽  
Gene Marker ◽  
Surface Markers ◽  
Differentiation Potential ◽  
Fat Depots ◽  
Reliable Source ◽  
Stromal Stem Cells

ABSTRACT The adipose tissue is a reliable source of Mesenchymal stem cells (MSCs) showing a higher plasticity and transdifferentiation potential into multilineage cells. In the present study, adipose tissue-derived mesenchymal stem cells (AT-MSCs) were isolated from mice omentum and epididymis fat depots. The AT-MSCs were initially compared based on stem cell surface markers and on the mesodermal trilineage differentiation potential. Additionally, AT-MSCs, from both sources, were cultured with differentiation media containing retinoic acid (RA) and/or testicular cell-conditioned medium (TCC). The AT-MSCs expressed mesenchymal surface markers and differentiated into adipogenic, chondrogenic and osteogenic lineages. Only omentum-derived AT-MSCs expressed one important gene marker related to male germ cell lineages, after the differentiation treatment with RA. These findings reaffirm the importance of adipose tissue as a source of multipotent stromal-stem cells, as well as, MSCs source regarding differentiation purpose.

scholarly journals Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem Cells: Alteration of Gene Expression after In Vitro Cell Culture

2005 ◽  
Vol 16 (3) ◽  
pp. 1131-1141 ◽  
Author(s):  
Andrew C. Boquest ◽  
Aboulghassem Shahdadfar ◽  
Katrine Frønsdal ◽  
Olafur Sigurjonsson ◽  
Siv H. Tunheim ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Histocompatibility Complex ◽  
Neural Tissues ◽  
Organ Specific ◽  
Stromal Stem Cells ◽  
Two Populations

Stromal stem cells proliferate in vitro and may be differentiated along several lineages. Freshly isolated, these cells have been too few or insufficiently pure to be thoroughly characterized. Here, we have isolated two populations of CD45-CD34+CD105+ cells from human adipose tissue which could be separated based on expression of CD31. Compared with CD31+ cells, CD31- cells overexpressed transcripts associated with cell cycle quiescence and stemness, and transcripts involved in the biology of cartilage, bone, fat, muscle, and neural tissues. In contrast, CD31+ cells overexpressed transcripts associated with endothelium and the major histocompatibility complex class II complex. Clones of CD31- cells could be expanded in vitro and differentiated into cells with characteristics of bone, fat, and neural-like tissue. On culture, transcripts associated with cell cycle quiescence, stemness, certain cytokines and organ specific genes were down-regulated, whereas transcripts associated with signal transduction, cell adhesion, and cytoskeletal components were up-regulated. CD31+ cells did not proliferate in vitro. CD45-CD34+CD105+CD31- cells from human adipose tissue have stromal stem cell properties which may make them useful for tissue engineering.

Osteoinductive effects of glyceollins on adult mesenchymal stromal/stem cells from adipose tissue and bone marrow

Phytomedicine ◽  
2017 ◽  
Vol 27 ◽  
pp. 39-51 ◽  
Author(s):  
Marjorie E. Bateman ◽  
Amy L. Strong ◽  
Ryan S. Hunter ◽  
Melyssa R Bratton ◽  
Rajesh Komati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Stromal Stem Cells
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