scholarly journals Effect of a Bone Marrow-Derived Extracellular Matrix on Cell Adhesion and Neural Induction of Dental Pulp Stem Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Samuele Laudani ◽  
Valentina La Cognata ◽  
Rosario Iemmolo ◽  
Gabriele Bonaventura ◽  
Giusy Villaggio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Dental Pulp ◽  
Neural Induction ◽  
Dental Pulp Stem Cells

scholarly journals Human dental pulp stem cells exhibit enhanced properties in comparison to human bone marrow stem cells on neurites outgrowth

2020 ◽  
Vol 34 (4) ◽  
pp. 5499-5511 ◽  
Author(s):  
Pierfrancesco Pagella ◽  
Shayee Miran ◽  
Estrela Neto ◽  
Ivan Martin ◽  
Meriem Lamghari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Dental Pulp ◽  
Enhanced Properties

Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation

2015 ◽  
Vol 20 (7) ◽  
pp. 076013 ◽  
Author(s):  
Hamideh Salehi ◽  
Pierre-Yves Collart-Dutilleul ◽  
Csilla Gergely ◽  
Frédéric J. G. Cuisinier
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Dental Pulp ◽  
Raman Microscopy ◽  
Dental Pulp Stem Cells ◽  
Confocal Raman Microscopy ◽  
Confocal Raman

Bone Marrow Versus Dental Pulp Stem Cells in Osteogenesis

2014 ◽  
pp. 127-141
Author(s):  
Mohammad Mahboob Kanafi ◽  
Sireesha Ganneru ◽  
Dhanasekaran Marappagounder ◽  
Padmanav Behera ◽  
Ramesh R. Bhonde
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells

scholarly journals Effect of extracellular matrix and dental pulp stem cells on bone regeneration with 3D printed PLA/HA composite scaffolds

2021 ◽  
Vol 41 ◽  
pp. 204-215
Author(s):  
I Gendviliene ◽  
◽  
E Simoliunas ◽  
M Alksne ◽  
S Dibart ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Negative Control ◽  
Dental Pulp Stem Cells ◽  
Female Rats ◽  
Micro Computed Tomography ◽  
Significant Difference ◽  
Gender Specific Differences

The demand for bone grafting procedures in various fields of medicine is increasing. Existing substitutes in clinical practice do not meet all the criteria required for an ideal bone scaffold, so new materials are being sought. This study evaluated bone regeneration using a critical-size Wistar rat’s calvarial defect model. 12 male and 12 female rats were evenly divided into 3 groups: 1. Negative and positive (Geistlich Bio-Oss®) controls; 2. polylactic acid (PLA) and PLA/hydroxyapatite (HA); 3. PLA/HA cellularised with dental pulp stem cells (DPSC) and PLA/HA extracellular matrix (ECM) scaffolds. PLA/HA filament was created using hot-melt extrusion equipment. All scaffolds were fabricated using a 3D printer. DPSC were isolated from the incisors of adult Wistar rats. The defects were evaluated by micro-computed tomography (µCT) and histology, 8 weeks after surgery. µCT revealed that the Bio-Oss group generated 1.49 mm3 and PLA/HA ECM 1.495 mm3 more bone volume than the negative control. Histology showed a statistically significant difference between negative control and both (Bio-Oss and PLA/HA ECM) groups in rats of both genders. Moreover, histology showed gender-specific differences in all experimental groups and a statistically significant difference between cellularised PLA/HA and PLA/HA ECM groups in female rats. Qualitative histology showed the pronounced inflammation reaction during biodegradation in the PLA group. In conclusion, the bone-forming ability was comparable between the Bio-Oss and PLA/HA ECM scaffolds. Further research is needed to analyse the effects of ECM and PLA/HA ratio on osteoregeneration.

scholarly journals Treatment of osteoradionecrosis of mandible with bone marrow concentrate and with dental pulp stem cells

2014 ◽  
Vol 4 (2) ◽  
pp. 189 ◽  
Author(s):  
S Sankaranarayanan ◽  
M Chandramohan ◽  
S Elangovan ◽  
K Manimaran ◽  
SMahendra Perumal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Marrow Concentrate

scholarly journals Comparison of the Expression of Periodontal Markers in Dental and Bone Marrow-derived Mesenchymal Stem Cells.

2020 ◽  
Vol 14 (1) ◽  
pp. 196-202
Author(s):  
Devy Garna ◽  
Manmeet Kaur ◽  
Francis J Hughes ◽  
Mandeep Ghuman
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Bone Marrow Cells ◽  
Dental Pulp Stem Cells ◽  
Periodontal Ligament Stem Cells ◽  
Osteogenic Induction ◽  
Pulp Cells

Background: Periodontal ligament stem cells are a source of mesenchymal stem cells, but it is unclear whether their phenotype is distinct from mesenchymal stem cells derived from different tissues, such as those derived from bone marrow. Objective: To investigate the expression of the putative PDL markers asporin, periostin, nestin and cementum protein 1, by periodontal ligament stem cells both constitutively and during osteogenic differentiation when compared to bone marrow-derived mesenchymal stem cells, and dental pulp stem cells. Methods: The primary human periodontal ligament, bone marrow, and dental pulp stem cells, and osteoblasts from different donors were cultured in vitro. The expression of periodontal marker associated genes during osteogenic induction was tested by qRT-PCR and immunofluorescence staining. Results: Asporin expression was detected in periodontal ligament stem cells and increased markedly during the time in culture (upregulated x53 fold at 21 days post-induction). During osteogenic differentiation, asporin expression significantly decreased in periodontal ligament cells whereas periostin significantly decreased in dental pulp cells. Periostin expression was absent in osteoblasts, but expression gradually increased in all other cells with time in culture. Nestin expression was mainly seen in the periodontal ligament and dental pulp cells and was largely absent in osteoblasts and bone marrow cells. Cementum protein-1 was most highly expressed in bone marrow cells and osteoblasts following osteogenic induction. Conclusions: The results provide further evidence that periodontal ligament-derived and bone marrow derived mesenchymal stem cells are phenotypically distinct. Periodontal markers are also expressed in dental pulp stem cells.

Expression of Extracellular Matrix Proteins in Human Dental Pulp Stem Cells Depends on the Donor Tooth Conditions

2010 ◽  
Vol 36 (5) ◽  
pp. 826-831 ◽  
Author(s):  
Sueli Patricia Harumi Miyagi ◽  
Irina Kerkis ◽  
Carlos Magno da Costa Maranduba ◽  
Cícera Maria Gomes ◽  
Manoela Domingues Martins ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Dental Pulp ◽  
Extracellular Matrix Proteins ◽  
Dental Pulp Stem Cells ◽  
Matrix Proteins ◽  
Human Dental Pulp
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