scholarly journals The Lysosomal Membrane Protein Lamp2 Alleviates Lysosomal Cell Death by Promoting Autophagic Flux in Ischemic Cardiomyocytes

2020 ◽  
Vol 8 ◽  
Author(s):  
Lin Cui ◽  
Li-Ping Zhao ◽  
Jing-Ying Ye ◽  
Lei Yang ◽  
Yao Huang ◽  
...  
Keyword(s):  
Cell Death ◽  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Autophagic Flux ◽  
Lysosomal Membrane Protein

scholarly journals The lysosomal membrane protein LAMP2A promotes autophagic flux and prevents SNCA-induced Parkinson disease-like symptoms in the Drosophila brain

Autophagy ◽  
2018 ◽  
Vol 14 (11) ◽  
pp. 1898-1910 ◽  
Author(s):  
Abdul-Raouf Issa ◽  
Jun Sun ◽  
Céline Petitgas ◽  
Ana Mesquita ◽  
Amina Dulac ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Parkinson Disease ◽  
Lysosomal Membrane ◽  
Autophagic Flux ◽  
Drosophila Brain ◽  
Lysosomal Membrane Protein

Expression of the lysosomal membrane protein LMBD1 is essential for the initiation of gastrulation

2017 ◽  
Vol 120 (1-2) ◽  
pp. S117
Author(s):  
Frank Rutsch ◽  
Petra Pennekamp ◽  
Yvonne Nitschke ◽  
Chrishanthi Lowe ◽  
Boris Skryabin ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein

scholarly journals Functional characterization of the lysosomal membrane protein TMEM192 in mice

Oncotarget ◽  
2017 ◽  
Vol 8 (27) ◽  
pp. 43635-43652 ◽  
Author(s):  
Thuy Linh Nguyen ◽  
Janna Schneppenheim ◽  
Sönke Rudnik ◽  
Renate Lüllmann-Rauch ◽  
Christian Bernreuther ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Functional Characterization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein

Comparative behavior of lysosomes and the pre-lysosome compartment (PLC) in in vivo cell fusion experiments

1991 ◽  
Vol 99 (3) ◽  
pp. 571-582 ◽  
Author(s):  
Y.P. Deng ◽  
G. Griffiths ◽  
B. Storrie
Keyword(s):  
Membrane Protein ◽  
Cell Fusion ◽  
Sindbis Virus ◽  
Single Cells ◽  
Mouse Cell ◽  
Lysosomal Membrane ◽  
Immunogold Electron Microscopy ◽  
Membrane Markers ◽  
Lysosomal Membrane Protein

Interspecies cell fusion was used to compare protein intermixing within the mannose 6-phosphate receptor (MPR)-enriched pre-lysosome compartment (PLC) and within the MPR-negative lysosomal compartment. Both compartments were positive for lysosomal glycoprotein (lgp) membrane markers but were morphologically distinct. In most experiments, rat-mouse cell syncytia were formed by u.v.-inactivated Sindbis virus-mediated fusion. By immunogold electron microscopy of syncytia, extensive intermixing of species-specific lysosomal membrane proteins was observed in both lysosomes and PLC. At 3 h post cell fusion, multiple-label immunogold studies showed that 82% of the lysosome-like structures positive for the rat lysosomal membrane protein LIMP-I were also positive for the mouse lysosomal membrane protein mLAMP-1. By immunofluorescence, LIMP-I and mLAMP-1 co-localized with a t1/2 of 30 min after cell fusion; although the lgp-positive organelle populations had evidently interchanged their proteins, the lysosomal structures remained small, punctate bodies distributed throughout the syncytoplasm as observed in single cells. In contrast, the initially separate units of the PLC congregated with a t1/2 of 1 h to form large, pre-lysosome complexes associated with individual nuclear clusters. At the electron-microscope level, gold markers endocytized by the rat and mouse parent cells in a 1 h uptake followed by a 16–20 h chase co-localized in these extended PLC complexes, as did the membrane markers mLAMP-1 and LIMP-I. The density of labeling for rat MPR in the extended PLCs was markedly decreased, consistent with membrane fusions and dilution of the antigen upon congregation of the PLC compartments from the donor cells. The extended PLC complex behaved as a late endocytic compartment, as shown by co-localization of the MPR and rhodamine-dextran following a 10 min dextran uptake and a 50 min chase. These differences in behavior between lysosomes and the PLC in rat-mouse cell syncytia suggest that the pathway(s) of protein intermixing with respect to the two organelles may be different.

scholarly journals Mucolipin-1 Is a Lysosomal Membrane Protein Required for Intracellular Lactosylceramide Traffic

Traffic ◽  
2006 ◽  
Vol 7 (10) ◽  
pp. 1388-1398 ◽  
Author(s):  
Paul R. Pryor ◽  
Frank Reimann ◽  
Fiona M. Gribble ◽  
J. Paul Luzio
Keyword(s):  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein

scholarly journals Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes

Autophagy ◽  
2017 ◽  
Vol 13 (1) ◽  
pp. 218-222 ◽  
Author(s):  
Shu Aizawa ◽  
Viorica Raluca Contu ◽  
Yuuki Fujiwara ◽  
Katsunori Hase ◽  
Hisae Kikuchi ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein
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