scholarly journals Genome-Wide Analysis of Circular RNAs Mediated ceRNA Regulation in Porcine Embryonic Muscle Development

2019 ◽  
Vol 7 ◽  
Author(s):  
Linjun Hong ◽  
Ting Gu ◽  
Yanjuan He ◽  
Chen Zhou ◽  
Qun Hu ◽  
...  
Keyword(s):  
Muscle Development ◽  
Circular Rnas ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Embryonic Muscle ◽  
Embryonic Muscle Development

scholarly journals Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens

DNA Research ◽  
2017 ◽  
Vol 25 (1) ◽  
pp. 71-86 ◽  
Author(s):  
Hongjia Ouyang ◽  
Xiaolan Chen ◽  
Zhijun Wang ◽  
Jiao Yu ◽  
Xinzheng Jia ◽  
...  
Keyword(s):  
Muscle Development ◽  
Circular Rnas ◽  
Embryonic Muscle ◽  
Embryonic Muscle Development

scholarly journals Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development

2021 ◽  
Vol 9 ◽  
Author(s):  
Baohua Tan ◽  
Sheng Wang ◽  
Shanshan Wang ◽  
Jiekang Zeng ◽  
Linjun Hong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Transcriptional Activation ◽  
Muscle Development ◽  
Global Scale ◽  
Genome Wide ◽  
H3k27me3 Level ◽  
Repressive Mark ◽  
Embryonic Muscle ◽  
Embryonic Muscle Development

The trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important chromatin modifications, which is generally presented as a repressive mark in various biological processes. However, the dynamic and global-scale distribution of H3K27me3 during porcine embryonic muscle development remains unclear. Here, our study provided a comprehensive genome-wide view of H3K27me3 and analyzed the matching transcriptome in the skeletal muscles on days 33, 65, and 90 post-coitus from Duroc fetuses. Transcriptome analysis identified 4,124 differentially expressed genes (DEGs) and revealed the key transcriptional properties in three stages. We found that the global H3K27me3 levels continually increased during embryonic development, and the H3K27me3 level was negatively correlated with gene expression. The loss of H3K27me3 in the promoter was associated with the transcriptional activation of 856 DEGs in various processes, including skeletal muscle development, calcium signaling, and multiple metabolic pathways. We also identified for the first time that H3K27me3 could enrich in the promoter of genes, such as DES, MYL1, TNNC1, and KLF5, to negatively regulate gene expression in porcine satellite cells (PSCs). The loss of H3K27me3 could promote muscle cell differentiation. Taken together, this study provided the first genome-wide landscape of H3K27me3 in porcine embryonic muscle development. It revealed the complex and broad function of H3K27me3 in the regulation of embryonic muscle development from skeletal muscle morphogenesis to myofiber maturation.

The mutant not enough muscles (nem) reveals reduction of the Drosophila embryonic muscle pattern

1995 ◽  
Vol 108 (4) ◽  
pp. 1443-1454 ◽  
Author(s):  
S. Burchard ◽  
A. Paululat ◽  
U. Hinz ◽  
R. Renkawitz-Pohl
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Muscle Development ◽  
P Element ◽  
Dual Function ◽  
Methane Sulfonate ◽  
Visceral Mesoderm ◽  
Embryonic Muscle ◽  
Embryonic Muscle Development ◽  
Somatic Musculature

In a search for mutations affecting embryonic muscle development in Drosophila we identified a mutation caused by the insertion of a P-element, which we called not enough muscles (nem). The phenotype of the P-element mutation of the nem gene suggests that it may be required for the development of the somatic musculature and the chordotonal organs of the PNS, while it is not involved in the development of the visceral mesoderm and the dorsal vessel. Mutant embryos are characterized by partial absence of muscles, monitored by immunostainings with mesoderm-specific anti-beta 3 tubulin and anti-myosin heavy chain antibodies. Besides these muscle distortions, defects in the peripheral nervous system were found, indicating a dual function of the nem gene product. Ethyl methane sulfonate-induced alleles for the P-element mutation were created for a detailed analysis. One of these alleles is characterized by unfused myoblasts which express beta 3 tubulin and myosin heavy chain, indicating the state of cell differentiation.

scholarly journals Genome-wide Analysis of Drosophila Circular RNAs Reveals Their Structural and Sequence Properties and Age-Dependent Neural Accumulation

Cell Reports ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. 1966-1980 ◽  
Author(s):  
Jakub O. Westholm ◽  
Pedro Miura ◽  
Sara Olson ◽  
Sol Shenker ◽  
Brian Joseph ◽  
...  
Keyword(s):  
Circular Rnas ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Age Dependent

scholarly journals Genome-Wide Analysis Reveals Extensive Changes in LncRNAs during Skeletal Muscle Development in Hu Sheep

Genes ◽  
2017 ◽  
Vol 8 (8) ◽  
pp. 191 ◽  
Author(s):  
Caifang Ren ◽  
Mingtian Deng ◽  
Yixuan Fan ◽  
Hua Yang ◽  
Guomin Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Skeletal Muscle Development ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Hu Sheep

scholarly journals Comparative Analysis of Skeletal Muscle DNA Methylation and Transcriptome of the Chicken Embryo at Different Developmental Stages

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinshan Ran ◽  
Jingjing Li ◽  
Lingqian Yin ◽  
Donghao Zhang ◽  
Chunlin Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Methylation ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Development ◽  
Muscle Satellite Cell ◽  
Genome Wide ◽  
Embryonic Muscle ◽  
Skeletal Muscle Satellite Cell ◽  
Chicken Skeletal Muscle

DNA methylation is a key epigenetic mechanism involved in embryonic muscle development and plays an important role in early muscle development. In this study, we sought to investigate the effects of genome-wide DNA methylation by combining the expression profiles of the chicken embryonic muscle. Genome-wide DNA methylation maps and transcriptomes of muscle tissues collected from different embryonic development points (E7, E11, E17, and D1) were used for whole-genome bisulfite sequencing (WGBS) and RNA sequencing, respectively. We found that the differentially methylated genes (DMGs) were significantly associated with muscle organ development, regulation of skeletal muscle satellite cell proliferation, and actin filament depolymerization. Furthermore, genes TBX1, MEF2D, SPEG, CFL2, and TWF2 were strongly correlated with the methylation-caused expression switch. Therefore, we chose the CFL2 gene to explore its function in skeletal muscle satellite cells, and the in vitro experiments showed that CFL2 acts as a negative regulator of chicken skeletal muscle satellite cell proliferation and can induce cell apoptosis. These results provide valuable data for future genome and epigenome studies of chicken skeletal muscle and may help reveal the molecular mechanisms of potential economic traits.

scholarly journals The Landscape of Chromatin Accessibility in Skeletal Muscle During Embryonic Development in Pigs

2020 ◽  
Author(s):  
Jingwei Yue ◽  
Xinhua Hou ◽  
Xin Liu ◽  
Ligang Wang ◽  
Hongmei Gao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Transcription Factors ◽  
Embryonic Development ◽  
Muscle Development ◽  
Chromatin Accessibility ◽  
Regulatory Role ◽  
Rna Seq ◽  
Skeletal Muscle Development ◽  
Embryonic Muscle ◽  
Embryonic Muscle Development

Abstract Background: The development of skeletal muscle during the embryonic stage in pigs is precisely regulated by transcriptional regulation, which depends on chromatin accessibility. However, how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been reported. To gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development, we performed ATAC-seq and RNA-seq on skeletal muscle of pig embryos at 45, 70 and 100 days post coitus (dpc). Results: In total, 21638, 35447 and 60181 unique regions (or peaks) were found across 45 dpc (LW45), 70 dpc (LW70) and 100 dpc (LW100) embryos, respectively. More than 91% of peaks were annotated within -1 kb to 100 bp of transcription start sites (TSSs). First, widespread increases in specific accessible chromatin regions (ACRs) from 45 to 100 dpc embryos suggested that the regulatory mechanisms became increasingly complicated during embryonic development. Second, the findings of integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the peak intensities of ACRs could control the expression of associated genes. Finally, motif screening of stage-specific ACRs revealed some transcription factors that regulated muscle development-related genes, such as MyoD, Mef2c, Mef2d and Pax7. Several potential transcriptional repressors, including E2F6, GRHL2, OTX2 and CTCF, were identified among those genes that exhibited different change trends between the ATAC-seq and RNA-seq data. Conclusions: This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription factors. Our results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development.

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