scholarly journals Determination of a Robust Assay for Human Sperm Membrane Potential Analysis

2019 ◽  
Vol 7 ◽  
Author(s):  
Carolina Baro Graf ◽  
Carla Ritagliati ◽  
Cintia Stival ◽  
Paula A. Balestrini ◽  
Mariano G. Buffone ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Human Sperm ◽  
Potential Analysis ◽  
Sperm Membrane

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

1990 ◽  
Vol 10 (2) ◽  
pp. 131-139
Author(s):  
Oyewole Adeyemo ◽  
E. O. Okegbile ◽  
O. O. Olorunsogo
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Partial Purification ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
Triton X

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

scholarly journals Calibration procedures for the quantitative determination of membrane potential in human cells using anionic dyes

2013 ◽  
Vol 83A (7) ◽  
pp. 612-626 ◽  
Author(s):  
Thomas Klapperstück ◽  
Dagobert Glanz ◽  
Stefan Hanitsch ◽  
Manuela Klapperstück ◽  
Fritz Markwardt ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Quantitative Determination ◽  
Human Cells ◽  
Anionic Dyes

DETERMINATION OF THE HUMAN SPERM DNA FRAGMENTATION INDEX

Vestnik ◽  
2021 ◽  
pp. 226-232
Author(s):  
К. К. Тлеуханов ◽  
Н. А. Алтыбаева ◽  
М. К. Отарбаев ◽  
Е. М. Тойшибеков ◽  
А. А. Тлеуханова
Keyword(s):  
Dna Fragmentation ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

В статье представлены собранные данные о методах устранения повышенной частоты фрагментации ДНК у сперматозоидов, которые в некоторых исследованиях подтверждают, что введение антиоксидантов может снизить уровень фрагментации ДНК у сперматозоидов. The article presents collected data on methods of eliminating the increased frequency of DNA fragmentation in spermatozoa, which in some studies confirm that the introduction of antioxidants can reduce the level of DNA fragmentation in spermatozoa.

scholarly journals Relevance of Fatty Acids to Sperm Maturation and Quality

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Giulia Collodel ◽  
Cesare Castellini ◽  
Jetty Chung-Yung Lee ◽  
Cinzia Signorini
Keyword(s):  
Biological Sciences ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Human Sperm ◽  
Dietary Fatty Acids ◽  
Pathological Conditions ◽  
Sperm Membrane ◽  
And Function

Almost 50% of infertility cases are associated with human male infertility. The sperm membrane is a key structure influencing sperm morphology and function in normal and pathological conditions. The fatty acid profile determines the performance not only of sperm motility but also of acrosomal reaction and sperm-oocyte fusion. This review presents available knowledge on the role of fatty acid composition in human sperm and spermatogenesis and discusses the influence of dietary fatty acids on the sperm fatty acid profile. Recent studies in biological sciences and clinical researches in this field are also reported. The topic object of this review has potential application in medicine by identifying potential causes of infertility.

scholarly journals The Effect of Kallikrein on Human Sperm Membrane Function.

1991 ◽  
Vol 164 (1) ◽  
pp. 23-28 ◽  
Author(s):  
NIKOLAOS SOFIKITIS ◽  
IKUO MIYAGAWA ◽  
TOSHIKO TODA ◽  
TASUKU HARADA ◽  
YASUYUKI MIO ◽  
...  
Keyword(s):  
Human Sperm ◽  
Membrane Function ◽  
Sperm Membrane

scholarly journals Screening of an Antigen Target for Immunocontraceptives from Cross-Reactive Antigens between Human Sperm and Ureaplasma urealyticum

2007 ◽  
Vol 75 (4) ◽  
pp. 2004-2011 ◽  
Author(s):  
Jianli Shi ◽  
Zhengmin Yang ◽  
Min Wang ◽  
Guoyan Cheng ◽  
Ding Li ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
Ureaplasma Urealyticum ◽  
Human Sperm ◽  
Epidemiologic Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antisperm Antibodies ◽  
Protein Database ◽  
Nuclear Autoantigenic Sperm Protein ◽  
Sperm Membrane ◽  
The Cross

ABSTRACT Epidemiologic studies indicated that some infertile men who were infected with Ureaplasma urealyticum displayed positive antisperm antibodies in their serum and/or semen. The purpose of this study was to investigate the possible mechanism of antisperm antibodies production after infection with U. urealyticum and to analyze the relationship between U. urealyticum and infertility. The existence of cross-reactive antigens (61, 50, and 25 kDa) between U. urealyticum and human sperm membrane proteins was confirmed. Among the cross-reactive antigens, the urease complex component UreG of U. urealyticum was determined. By searching the Swiss-Prot protein database, a pentapeptide identity (IERLT) between UreG and human nuclear autoantigenic sperm protein (NASP) was found. Furthermore, using Western blot analysis and enzyme-linked immunosorbent assay, the cross-reaction between the NASP and UreG was verified. Both anti-rUreG antibody and the antiserum against the synthetic peptide NASP393-408 containing the pentapeptide inhibited mouse sperm egg binding and fusion. After immunization by rUreG or the synthetic peptide, 81.2 and 75% female mice became sterile, respectively. The effect on fertility in mice immunized with the synthetic peptide was reversible. These findings proved for the first time that it was feasible to screen antigens for immunocontraceptives from cross-reactive antigens between sperm and microorganisms which induce infertility.

Simultaneous determination of membrane potential and pH gradient by photodiode array spectroscopy

1986 ◽  
Vol 862 (2) ◽  
pp. 278-284 ◽  
Author(s):  
Tetsuya Konishi ◽  
Naoyuki Murakami ◽  
Yoshihiko Hatano ◽  
Katsuyoshi Nakazato
Keyword(s):  
Membrane Potential ◽  
Simultaneous Determination ◽  
Ph Gradient ◽  
Photodiode Array
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