scholarly journals Quantitative Analysis of Interaction Between CADM1 and Its Binding Cell-Surface Proteins Using Surface Plasmon Resonance Imaging

2018 ◽  
Vol 6 ◽  
Author(s):  
Takeshi Ito ◽  
Yutaka Kasai ◽  
Yuki Kumagai ◽  
Daisuke Suzuki ◽  
Misaki Ochiai-Noguchi ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Quantitative Analysis ◽  
Cell Surface ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Proteins ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Cell Surface Proteins ◽  
Binding Cell

scholarly journals Multiplex Label Free Characterization of Cancer Cell Lines Using Surface Plasmon Resonance Imaging

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 70 ◽  
Author(s):  
Ivan Stojanović ◽  
Carolina F. Ruivo ◽  
Thomas J. G. van der Velden ◽  
Richard B. M. Schasfoort ◽  
Leon W. M. M. Terstappen
Keyword(s):  
Flow Cytometry ◽  
Surface Plasmon Resonance ◽  
Cell Surface ◽  
Cell Lines ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Marker ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Surface Marker Analysis

Rapid multiplex cell surface marker analysis can expedite investigations in which large number of antigens need to be analyzed. Simultaneous analysis of multiple surface antigens at the same level of sensitivity is however limited in the current golden standard analysis method, flow cytometry. In this paper we introduce a surface plasmon resonance imaging (SPRi)-based technique for 44-plex parameter analysis using a single sample, in less than 20 min. We analyzed the expression on cells from five different cancer cell lines by SPRi on a 44-plex antibody array including 4 negative controls and compared the output with flow cytometry. The combined correlation of the markers that showed expression by flow cytometry was 0.76. The results demonstrate as a proof of principle that SPRi can be applied for rapid semi-quantitative multiplex cell surface marker analysis.

Lectin recognition of a new SOD mimic bioconjugate studied with surface plasmon resonance imaging

2006 ◽  
Vol 4 (4) ◽  
pp. 610 ◽  
Author(s):  
Roberta D'Agata ◽  
Giulia Grasso ◽  
Giuseppe Iacono ◽  
Giuseppe Spoto ◽  
Graziella Vecchio
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Lectin Recognition

Integrated Electrokinetic Sample Focusing and Surface Plasmon Resonance Imaging System for Measuring Biomolecular Interactions

2009 ◽  
Vol 81 (5) ◽  
pp. 1957-1963 ◽  
Author(s):  
Ganeshram Krishnamoorthy ◽  
Edwin T. Carlen ◽  
Dietrich Kohlheyer ◽  
Richard B. M. Schasfoort ◽  
Albert van den Berg
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Imaging System ◽  
Biomolecular Interactions ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

Parallel scan spectral surface plasmon resonance imaging

2008 ◽  
Vol 47 (30) ◽  
pp. 5616 ◽  
Author(s):  
Le Liu ◽  
Yonghong He ◽  
Ying Zhang ◽  
Suihua Ma ◽  
Hui Ma ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Parallel Scan
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