scholarly journals Anaphase Chromosomes in Crane-Fly Spermatocytes Treated With Taxol (Paclitaxel) Accelerate When Their Kinetochore Microtubules Are Cut: Evidence for Spindle Matrix Involvement With Spindle Forces

2018 ◽  
Vol 6 ◽  
Author(s):  
Arthur Forer ◽  
Rozhan Sheykhani ◽  
Michael W. Berns
Keyword(s):  
Spindle Matrix

scholarly journals Erratum: An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Song Li ◽  
Chwee Tat Koe ◽  
Su Ting Tay ◽  
Angie Lay Keng Tan ◽  
Shenli Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Matrix Proteins ◽  
Spindle Matrix ◽  
Intrinsic Mechanism

scholarly journals Evidence for a role of spindle matrix formation in cell cycle progression by antibody perturbation

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0208022 ◽  
Author(s):  
Changfu Yao ◽  
Chao Wang ◽  
Yeran Li ◽  
Michael Zavortink ◽  
Vincent Archambault ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Cycle Progression ◽  
Spindle Matrix

scholarly journals The spindle matrix through the cell cycle in Drosophila

Fly ◽  
2009 ◽  
Vol 3 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Jorgen Johansen ◽  
Kristen M. Johansen
Keyword(s):  
Cell Cycle ◽  
Spindle Matrix

scholarly journals Eg5 is static in bipolar spindles relative to tubulin

2001 ◽  
Vol 154 (6) ◽  
pp. 1125-1134 ◽  
Author(s):  
Tarun M. Kapoor ◽  
Timothy J. Mitchison
Keyword(s):  
Motor Activity ◽  
Half Life ◽  
Microtubule Dynamics ◽  
Spindle Matrix ◽  
Spindle Poles ◽  
Kinesin Eg5 ◽  
Mitotic Kinesin Eg5 ◽  
Bipolar Spindles

We used fluorescent speckle microscopy to probe the dynamics of the mitotic kinesin Eg5 in Xenopus extract spindles, and compared them to microtubule dynamics. We found significant populations of Eg5 that were static over several seconds while microtubules flux towards spindle poles. Eg5 dynamics are frozen by adenylimidodiphosphate. Bulk turnover experiments showed that Eg5 can exchange between the spindle and the extract with a half life of <55 s. Eg5 distribution in spindles was not perturbed by inhibition of its motor activity with monastrol, but was perturbed by inhibition of dynactin with p50 dynamitin. We interpret these data as revealing the existence of a static spindle matrix that promotes Eg5 targeting to spindles, and transient immobilization of Eg5 within spindles. We discuss alternative interpretations of the Eg5 dynamics we observe, ideas for the biochemical nature of a spindle matrix, and implications for Eg5 function.

Cell and Molecular Biology of the Spindle Matrix

2007 ◽  
pp. 155-206 ◽  
Author(s):  
Kristen M. Johansen ◽  
Jørgen Johansen
Keyword(s):  
Molecular Biology ◽  
Spindle Matrix

scholarly journals Chromosome motion and the spindle matrix.

1984 ◽  
Vol 99 (1) ◽  
pp. 137s-143s ◽  
Author(s):  
J Pickett-Heaps ◽  
T Spurck ◽  
D Tippit
Keyword(s):  
Spindle Matrix ◽  
Chromosome Motion

Chromator, a novel and essential chromodomain protein interacts directly with the putative spindle matrix protein skeletor

2004 ◽  
Vol 93 (5) ◽  
pp. 1033-1047 ◽  
Author(s):  
Uttama Rath ◽  
Dong Wang ◽  
Yun Ding ◽  
Ying-Zhi Xu ◽  
Hongying Qi ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Spindle Matrix

scholarly journals Recent Glimpses of the Elusive Spindle Matrix

Cell Cycle ◽  
2002 ◽  
Vol 1 (5) ◽  
pp. 312-314 ◽  
Author(s):  
Kristen M. Johansen ◽  
Jorgen Johansen
Keyword(s):  
Spindle Matrix

scholarly journals Megator, an Essential Coiled-Coil Protein that Localizes to the Putative Spindle Matrix during Mitosis inDrosophila

2004 ◽  
Vol 15 (11) ◽  
pp. 4854-4865 ◽  
Author(s):  
Hongying Qi ◽  
Uttama Rath ◽  
Dong Wang ◽  
Ying-Zhi Xu ◽  
Yun Ding ◽  
...  
Keyword(s):  
Coiled Coil ◽  
Matrix Proteins ◽  
P Element ◽  
Structural Basis ◽  
Spindle Matrix ◽  
Terminal Domain ◽  
Coiled Coil Domain ◽  
Coiled Coil Region ◽  
Spherical Structures ◽  
Spindle Structure

We have used immunocytochemistry and cross-immunoprecipitation analysis to demonstrate that Megator (Bx34 antigen), a Tpr ortholog in Drosophila with an extended coiled-coil domain, colocalizes with the putative spindle matrix proteins Skeletor and Chromator during mitosis. Analysis of P-element mutations in the Megator locus showed that Megator is an essential protein. During interphase Megator is localized to the nuclear rim and occupies the intranuclear space surrounding the chromosomes. However, during mitosis Megator reorganizes and aligns together with Skeletor and Chromator into a fusiform spindle structure. The Megator metaphase spindle persists in the absence of microtubule spindles, strongly implying that the existence of the Megator-defined spindle does not require polymerized microtubules. Deletion construct analysis in S2 cells indicates that the COOH-terminal part of Megator without the coiled-coil region was sufficient for both nuclear as well as spindle localization. In contrast, the NH2-terminal coiled-coil region remains in the cytoplasm; however, we show that it is capable of assembling into spherical structures. On the basis of these findings we propose that the COOH-terminal domain of Megator functions as a targeting and localization domain, whereas the NH2-terminal domain is responsible for forming polymers that may serve as a structural basis for the putative spindle matrix complex.

Sign in / Sign up

Export Citation Format

Share Document