scholarly journals The Cells and Extracellular Matrix of Human Amniotic Membrane Hinder the Growth and Invasive Potential of Bladder Urothelial Cancer Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Taja Železnik Ramuta ◽  
Urška Dragin Jerman ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Marta Magatti ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cancer Cells ◽  
Amniotic Membrane ◽  
Urothelial Cancer ◽  
Human Amniotic Membrane ◽  
Invasive Potential

scholarly journals Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 606
Author(s):  
Dilcele Silva Moreira Dziedzic ◽  
Bassam Felipe Mogharbel ◽  
Ana Carolina Irioda ◽  
Priscila Elias Ferreira Stricker ◽  
Maiara Carolina Perussolo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Cell Attachment ◽  
Periodontal Regeneration ◽  
Human Amniotic Membrane ◽  
Micro Computed Tomography ◽  
Adipose Derived Stromal Cells

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

scholarly journals Angiogenic potential of extracellular matrix of human amniotic membrane

2016 ◽  
Vol 13 (3) ◽  
pp. 211-217 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
Thirumulu Ponnuraj Kannan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Angiogenic Potential

scholarly journals Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models

2021 ◽  
Vol 9 ◽  
Author(s):  
Aleksandar Janev ◽  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Žiga Sardoč ◽  
Hristina Obradović ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Amniotic Membrane ◽  
Detrimental Effect ◽  
Human Amniotic Membrane ◽  
Urothelial Cells ◽  
Papillary Cancer ◽  
Cancer Models ◽  
Bladder Cancer Cells ◽  
2D And 3D

Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.

scholarly journals Human Amniotic Membrane Enriched with Urinary Bladder Fibroblasts Promote the Re-Epithelization of Urothelial Injury

2020 ◽  
Vol 29 ◽  
pp. 096368972094666
Author(s):  
Urška Dragin Jerman ◽  
Peter Veranič ◽  
Tina Cirman ◽  
Mateja Erdani Kreft
Keyword(s):  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
Wound Dressing ◽  
Ex Vivo ◽  
Cell Junctions ◽  
Human Amniotic Membrane ◽  
Urothelial Cells ◽  
Ex Vivo Model ◽  
Bladder Tissue ◽  
Extracellular Matrix Components

Culturing cells in three-dimensional systems that include extracellular matrix components and different cell types mimic the native tissue and as such provide much more representative results than conventional two-dimensional cell cultures. In order to develop biomimetic bladder tissue in vitro, we used human amniotic membrane (AM) extracellular matrix as a scaffold for bladder fibroblasts (BFs) and urothelial cells. Our aims were to evaluate the integration of BFs into the AM stroma, to assess the differentiation of the urothelium on BFs-enriched AM scaffolds, and to evaluate the AM as a urothelial wound dressing. First, to achieve the optimal integration of BFs into AM stroma, different intact and de- epithelialized AM (dAM) scaffolds were tested. BFs secreted matrix metalloproteinase (MMP)-1 and MMP-2 and integrated into the stroma of all types of AM scaffolds. Second, to establish urothelial tissue equivalent, urothelial cells were seeded on dAM scaffolds enriched with BFs. The BFs in the stroma of the AM scaffolds promoted (1) the proliferation of urothelial cells, (2) the attachment of urothelial cells on AM basal lamina with hemidesmosomes, and (3) development of multilayered urothelium with expressed uroplakins and well-developed cell junctions. Third, we established an ex vivo model of the injured bladder to evaluate the dAM as a wound dressing for urothelial full-thickness injury. dAM acted as a promising wound dressing since it enabled rapid re-epithelization of urothelial injury and integrated into the bladder tissue. Herein, the developed urothelial tissue equivalents enable further mechanistic studies of bladder epithelial–mesenchymal interactions, and they could be applied as biomimetic models for preclinical testing of newly developed drugs. Moreover, we could hypothesize that AM may be suitable as a dressing of the wound that occurs during transurethral resection of bladder tumor, since it could diminish the possibility of tumor recurrence, by promoting the rapid re-epithelization of the urothelium.

scholarly journals Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy

2021 ◽  
Vol 20 ◽  
pp. 153303382110363
Author(s):  
Ameneh Jafari ◽  
Mostafa Rezaei-Tavirani ◽  
Hassan Niknejad ◽  
Hakimeh Zali
Keyword(s):  
Breast Cancer ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Conditioned Medium ◽  
Amniotic Membrane ◽  
Breast Cancer Cells ◽  
Migration And Invasion ◽  
Human Amniotic Membrane ◽  
Dependent Manner ◽  
Breast Cancer Therapy

Objectives: Traditional breast cancer treatments have challenges including inefficiency, multidrug resistance, severe side effects, and targeting non-specifically. The development of alternative treatment strategies has attracted a great deal of interest. Using the amniotic membrane has become a promising and convenient new approach for cancer therapy. This study aimed to evaluate the anti-cancer ability of conditioned medium extracted from the human amniotic membrane (hAM-CM) on breast cancer cells. Methods: Conditioned medium was collected after 48 h incubation of hAM in epithelial up manner. MTT, cell cycle, apoptosis, colony formation, and sphere assays were used to determine the impact of hAM-CM on breast cancer cell lines. The effects of hAM-CM on the migration and invasion of breast cancer cells were determined using scratch wound healing and transwell assays, respectively. Results: Based on the results, cell viability was significantly decreased by hAM-CM in a dose-dependent manner. The hAM-CM remarkably induced apoptosis and necrosis of cancer cells. Moreover, cell migration and invasion potential of cancer cells decreased after the hAM-CM treatment. Further, both the number of colonies and their morphologies were affected by the treatment. In the treated group, a significant decrease in the number of colonies along with an obvious change in their morphologies from holoclone shape to a dominant paracolone structure was observed. Conclusion: Our results indicate that the conditioned medium derived from the human amniotic membrane able to inhibit proliferation and metastasis of tumor cells and can be considered a natural and valuable candidate for breast cancer therapy.

scholarly journals Tumor–Stroma Cross-Talk in Human Pancreatic Ductal Adenocarcinoma: A Focus on the Effect of the Extracellular Matrix on Tumor Cell Phenotype and Invasive Potential

Cells ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 158 ◽  
Author(s):  
Patrizia Procacci ◽  
Claudia Moscheni ◽  
Patrizia Sartori ◽  
Michele Sommariva ◽  
Nicoletta Gagliano
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Cancer Cells ◽  
Ductal Adenocarcinoma ◽  
Cell Phenotype ◽  
Type I ◽  
Invasive Potential ◽  
Emt Markers ◽  
E Cadherin

The extracellular matrix (ECM) in the tumor microenvironment modulates the cancer cell phenotype, especially in pancreatic ductal adenocarcinoma (PDAC), a tumor characterized by an intense desmoplastic reaction. Because the epithelial-to-mesenchymal transition (EMT), a process that provides cancer cells with a metastatic phenotype, plays an important role in PDAC progression, the authors aimed to explore in vitro the interactions between human PDAC cells and ECM components of the PDAC microenvironment, focusing on the expression of EMT markers and matrix metalloproteinases (MMPs) that are able to digest the basement membrane during tumor invasion. EMT markers and the invasive potential of HPAF-II, HPAC, and PL45 cells grown on different ECM substrates (fibronectin, laminin, and collagen) were analyzed. While N-cadherin, αSMA, and type I collagen were not significantly affected by ECM components, the E-cadherin/β-catenin complex was highly expressed in all the experimental conditions, and E-cadherin was upregulated by collagen in PL45 cells. Cell migration was unaffected by fibronectin and delayed by laminin. In contrast, collagen significantly stimulated cell migration and the secretion of MMPs. This study’s results showed that ECM components impacted cell migration and invasive potential differently. Collagen exerted a more evident effect, providing new insights into the understanding of the intricate interplay between ECM molecules and cancer cells, in order to find novel therapeutic targets for PDAC treatment.

scholarly journals Could human amniotic membrane be a source for acupoint thread embedding therapy?

2021 ◽  
Vol 64 (6) ◽  
pp. 41-48
Author(s):  
Olga Ignatov ◽  
◽  
Adrian Melnic ◽  
Vitalie Procopciuc ◽  
Viorica Mihaluta ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Amniotic Membrane ◽  
Subcutaneous Tissue ◽  
Biomechanical Properties ◽  
Functional Restoration ◽  
Matrix Proteins ◽  
Human Amniotic Membrane ◽  
Ethical Problems ◽  
Motor Disability ◽  
Embedding Therapy

Background: Peripheral neuropathy usually leads to a major cause of motor disability, but the functional restoration after treatment continues to show modest results. Acupoint thread-embedding therapy is a subtype of acupuncture treatment in which biodegradable threads are inserted into skin, subcutaneous tissue or muscles at specific points for long stimulation. Different biodegradable materials have been developed and widely used. Human amniotic membrane is rich in collagen, extracellular matrix proteins and growth factors. The avascular, low immunogenic, anti-inflammatory, antibacterial, anti-fibrotic and non-tumorigenic properties of amniotic membrane make it valuable in medical applications and its use has no ethical problems. Elasticity, stiffness and other biomechanical properties also make it possible to use the amniotic membrane for various medical purposes. AM is almost always considered as discarded substance, it satisfies most of the criteria of an ideal biological tissue and shows almost zero rejection phenomenon. Conclusions: The human amniotic membrane, the cellular compounds and extracellular matrix have a lot of benefic proprieties that are or could be used in treatment of many human diseases. Its biological and biomechanical properties are promising in the manufacture and use of filaments in acupoint thread embedding therapy.

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